T4 Polynucleotide Kinase

Name: T4 Polynucleotide Kinase
SKU: EMQZ1405
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMQZ1405

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Product Information

Cat. No.	EMQZ1405
Description	Catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´ -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3´-monophosphates. Polynucleotide Kinase also catalyzes the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates.
Source	A recombinant E. coli strain that carries the cloned T4 Polynucleotide Kinase gene.
Concentration	10,000U/ml
Applications	• End-labeling DNA or RNA for probes and DNA sequencing • Addition of 5´-phosphates to oligonucleotides to allow subsequent ligation • Removal of 3´-phosphoryl groups
Size	200U; 1,000U
Unit Definition	One Richardson unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of acid-insoluble [32P] in 30 minutes at 37°C.
Reaction Conditions	1×T4 Polynucleotide Kinase Reaction System containing: 1×T4 Polynucleotide Kinase Reaction Buffer [70 mM Tris-HCl (pH 7.6 at 25°C),10 mM MgCl2,5 mM DTT]; incubate at 37°C.
Storage	Store at -20°C

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Customer Reviews

Customer Q&As

What is the concentration of this reagent?

A: The concentration of this reagent is 10,000 U/ml.

Can it catalyze the formation of a phosphate group from a nucleotide?

A: Yes, catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5´ -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3´-monophosphates。

What reagents are included in this product?

A: There are two reagents included in this product, Enzyme Solution and Phosphorylation Buffer 10x concentrated.

How should this reagent be stored?

A: The reagent can be stored directly at -20℃.

Where does this enzyme come from?

A: The enzyme is expressed and purified from Escherichia coli, and its expression gene is derived from T4 bacteriophage.

How can the activity of this enzyme be inhibited?

A: Heating at 75℃ for 10 minutes can inactivate T4 Polynucleotide Kinase, and metal ion chelators, phosphates, ammonium ions, KCl and NaCl greater than 50mM can significantly inhibit the activity of T4 Polynucleotide Kinase.

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Customer Reviews

Removes 3'-diphosphates

T4 Polynucleotide Kinase catalyzes the removal of 3´-phosphoryl groups from 3´-phosphoryl polynucleotides, deoxynucleoside 3´-monophosphates and deoxynucleoside 3´-diphosphates.

United States

2020-01-12

Phosphorylation

This reagent not only catalyzes 5'-hydroxyl terminal phosphorylation of polynucleotides, but also catalyzes phosphorylation of nucleoside 3'-monophosphates.

United States

2021-04-11

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T4 Polynucleotide Kinase

Product Information

Q & A

Customer Reviews

What is the concentration of this reagent?

Can it catalyze the formation of a phosphate group from a nucleotide?

What reagents are included in this product?

How should this reagent be stored?

Where does this enzyme come from?

How can the activity of this enzyme be inhibited?

Removes 3'-diphosphates

Phosphorylation

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