Panoply™ Human HSP90AA1 Knockdown Stable Cell Line

Name: Panoply™ Human HSP90AA1 Knockdown Stable Cell Line
SKU: CSC-DC007301
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC007301

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

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Gene Information

Cat. No.	CSC-DC007301
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	HSP90AA1
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	HSP90AA1 heat shock protein 90kDa alpha (cytosolic), class A member 1 [ Homo sapiens ]
Gene Symbol	HSP90AA1
Synonyms	HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4
Gene Description	heat shock protein 90kDa alpha (cytosolic), class A member 1
GeneID	3320
Uni ProtID	P07900
mRNA Refseq	NM_001017963.2
Protein Refseq	NP_001017963.2
Chromosome Location	14q32.33
Function	ATP binding; ATPase activity; TPR domain binding; TPR domain binding; identical protein binding; nitric-oxide synthase regulator activity; nucleotide binding; protein binding; protein homodimerization activity; unfolded protein binding;
Pathway	Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Axon guidance, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Centrosome maturation, organism-specific biosystem; Class I PI3K signaling events, organism-specific biosystem;
MIM	140571

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HSP90AA1 is a chaperone protein involved in various biological processes, including inflammation and cancer. HSP90AA1 is highly expressed in lung adenocarcinoma (LUAD), but its exact function remains unclear. Here, prognostic analysis of survival data from the TCGA database showed that HSP90AA1 expression is associated with clinicopathological staging. The overall survival (OS) and disease-specific survival (DSS) were significantly higher in the low-expression group of HSP90AA1 compared to the high-expression group. Furthermore, CancerSEA functional similarity analysis revealed that HSP90AA1 is involved in multiple cellular functions, including cell cycle stimulation, DNA damage response, invasion, and proliferation. Analysis of immune scores and immune cell infiltration using the ESTIMATE and TIMER databases showed that high levels of HSP90AA1 were associated with decreased infiltration of CD8+ T cells and plasmacytoid dendritic cells (pDCs), while infiltration of Th2 cells and helper T cells was increased. In vitro experiments further confirmed that knockdown of HSP90AA1 expression significantly inhibited the proliferation of H1299 cells. These findings suggest that silencing HSP90AA1 expression or using HSP90AA1 inhibitors could effectively improve the treatment of LUAD. Targeting HSP90AA1 may be an effective strategy for treating LUAD.

Compared to the control group, the proliferation of HSP90AA1-knockdown H1299 cells was inhibited (Figure 1C). To confirm the reason for the inhibited cell proliferation, researchers used PI staining to detect the cell cycle of H1299 cells. The results showed that, compared to the control group, the number of cells in the G1 phase increased, while the number of cells in the S and G2/M phases decreased in HSP90AA1-knockdown cells (Figure 1D-G). This indicates that the absence of HSP90AA1 leads to H1299 cell cycle arrest in the G1 phase. Furthermore, Western blot experiments confirmed that knocking down HSP90AA1 effectively downregulated the expression of cell cycle-related proteins CCNB1 and CCND1 (Figure 1H, I). These findings suggest that HSP90AA1 may regulate the cell cycle by modulating these specific proteins.

Figure 1. HSP90AA1 is crucial for the proliferation of LUAD cells. (Xiang S, et al., 2025)

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