Panoply™ Human HSP90AA1 Over-expressing Stable Cell Line

Recent studies have shown that heat shock protein 90 alpha family class A member 1 (HSP90AA1) interacts with various tumor-related proteins, regulating their biological activity and stability, and plays an important role in various cancers. However, the role of HSP90AA1 in clear cell renal cell carcinoma (ccRCC) remains unclear. Here, researchers demonstrated that HSP90AA1 is significantly downregulated in ccRCC, and its reduced expression is associated with tumor metastasis. Overexpression of HSP90AA1 significantly inhibited the proliferation and metastasis of ccRCC cells. HSP90AA1 binds to F-box protein 7 (FBXO7) and promotes its protein expression. FBXO7 is downregulated in ccRCC, and its reduced expression is closely associated with unfavorable pathological features and poor prognosis in patients. Overexpression of FBXO7 promotes the expression of cell adhesion molecule 1 (CADM1) and inhibits the PI3K-AKT signaling pathway. Knocking down FBXO7 expression in the context of HSP90AA1 overexpression significantly reversed the inhibitory effect on cell phenotype caused by HSP90AA1 overexpression, downregulated CADM1 expression, and activated the PI3K-AKT signaling pathway. In summary, HSP90AA1 is downregulated in ccRCC, and HSP90AA1 overexpression promotes CADM1 expression and inhibits the PI3K-AKT pathway, thereby suppressing the proliferation and metastasis of ccRCC.

Here, researchers constructed HSP90AA1-overexpressing OSRC2 and Caki-1 cell lines (Figure 1F). Plate cloning assays showed that the growth ability of HSP90AA1-overexpressing cells was significantly reduced compared to the control group (Figure 1A). EdU and TUNEL fluorescence detection results showed that after HSP90AA1 overexpression, ccRCC cell proliferation was significantly reduced, and the number of apoptotic cells significantly increased (Figure 1B). Cell migration and invasion experiments also showed that HSP90AA1 overexpression significantly inhibited the invasive ability of ccRCC cells (Figure 1C, D). Furthermore, researchers constructed a mouse renal orthotopic xenograft tumor growth model by injecting control or HSP90AA1-overexpressing Caki-1 cells under the renal capsule of mice. Bioluminescence imaging data analysis confirmed that the renal tumor growth rate in the HSP90AA1 overexpression group was significantly lower than that in the control group (Figure 1E). Then, H&E staining was used to detect the pathological structure of the mouse renal tumors (Figure 1F). Subsequently, cell proliferation and apoptosis in the renal tumors were detected using EdU and TUNEL methods, respectively. Compared with the control group, EdU expression was significantly reduced in the HSP90AA1 overexpression group, while TUNEL and Cleaved-Caspase 3 expression were significantly increased. These results fully demonstrate the inhibitory effect of HSP90AA1 overexpression on ccRCC cell proliferation and metastasis.

Figure 1. HSP90AA1 overexpression inhibited the proliferation and metastasis of ccRCC cells in vitro and in vivo. (Yang W, et al., 2026)