Panoply™ Human GPNMB Knockdown Stable Cell Line

Name: Panoply™ Human GPNMB Knockdown Stable Cell Line
SKU: CSC-DC006505
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-DC006505

Host Cell : HEK293 (Hela and other cell types are also available) Validation : Real-Time RCR

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Cell Line Information

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Gene Information

Cat. No.	CSC-DC006505
Description	Creative Biogene's Knockdown Cell Lines are target specific shRNA lentivirus transduced cells. The percent knockdown levels range from 75-99% depending on the gene, as evaluated by Real-Time RCR. Cells are rigorously qualified and mycoplasma free.
Target Gene	GPNMB
Host Cell	HEK293 (Hela and other cell types are also available)
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid Nitrogen
Shipping	Dry Ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GPNMB glycoprotein (transmembrane) nmb [ Homo sapiens ]
Gene Symbol	GPNMB
Synonyms	NMB; HGFIN
Gene Description	glycoprotein (transmembrane) nmb
Gene ID	10457
Uni Prot ID	Q14956
m RNA Refseq	NM_002510.2
Protein Refseq	NP_002501.1
Chromosome Location	7p15
Function	heparin binding; integrin binding;
MIM	604368

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Glycoprotein non-metastatic protein B (GPNMB) promotes bone metastasis (BM) in various cancers. However, the expression and function of GPNMB in patients with renal cell carcinoma (RCC) and BM remain unclear. Here, researchers investigated the clinical significance and biological function of GPNMB in RCC with BM. Results showed that high GPNMB expression levels were significantly correlated with the number and extent of BM, Fuhrman grade, and ERK expression in the primary tumor. Furthermore, GPNMB overexpression was significantly associated with poor prognosis (overall survival). Furthermore, researchers generated a GPNMB knockdown ACHN cell line. Low GPNMB expression inhibited RCC cell proliferation, as measured using the Cell Counting Kit-8 assay. Compared to cells transduced with negative control shRNA, GPNMB knockdown of renal cell carcinoma cells exhibited significantly reduced cell migration and invasion. Furthermore, phosphorylated ERK protein expression was lower in GPNMB knockdown ACHN cells compared to control cells. These results suggest that GPNMB plays an important role in tumor progression in renal cell carcinoma with bone marrow metastasis. It can serve as a predictive marker for bone marrow metastasis and a poor prognostic factor for renal cell carcinoma with bone marrow metastasis. GPNMB downregulation inhibits the proliferation, migration, and invasion of renal cell carcinoma, possibly by inhibiting the ERK signaling pathway.

Here, researchers investigated the effects of GPNMB knockdown on RCC cell proliferation. Western blot analysis showed that GPNMB protein expression levels were suppressed after shRNA transduction (Figure 1A). CCK-8 assays revealed that the cell proliferation capacity of GPNMB knockdown cells was significantly reduced compared with the NC group (Figure 1B). To investigate the invasion and migration capacity of GPNMB knockdown RCC cells, Transwell and Matrigel assays were performed (Figure 1C). Transwell assay results showed that the migration capacity of the GPNMB knockdown ACHN cell line was significantly inhibited compared with the control cells (Figure 1D). In addition, Matrigel assay results showed that the number of invasive cells in the GPNMB knockdown ACHN cell line was significantly lower than that in the control group (Figure 1D).

Figure 1. Effect of GPNMB downregulation. (Zhai J P, et al., 2022)

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