Panoply™ Human CD248 Over-expressing Stable Cell Line

Name: Panoply™ Human CD248 Over-expressing Stable Cell Line
SKU: CSC-SC002724
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC002724

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC002724
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	CD248
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD248 CD248 molecule, endosialin [ Homo sapiens ]
Gene Symbol	CD248
Synonyms	TEM1; CD164L1
Gene Description	CD248 molecule, endosialin
Gene ID	57124
Uni Prot ID	Q9HCU0
m RNA Refseq	NM_020404.2
Protein Refseq	NP_065137.1
Chromosome Location	11q13
Function	calcium ion binding; carbohydrate binding; molecular_function;
MIM	606064

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Tumor immune evasion is a key step in tumor progression. Cancer-associated fibroblasts (CAFs) within the tumor microenvironment (TME) express abundant PD-L1 and suppress CD8+ T cell function, thereby enabling immune evasion. CD248 is a candidate biomarker for non-small cell lung cancer (NSCLC)-associated CAFs, although its role in immune evasion remains unclear. Here, researchers demonstrate that CD248 increases PD-L1 levels in CAFs, inhibits CD8+ T cell function, and promotes NSCLC cell invasion and migration. CD248-induced activation of the FAK/Src/JNK/c-Jun axis promotes PD-L1 expression on CAFs. In tumor-bearing mice, lung cancer growth is significantly slowed, and fibroblast-specific CD248 knockout mice have higher numbers of granzyme B+ CD8+ T cells than wild-type mice. Importantly, the efficacy of tislelizumab is enhanced in CD248 knockout mice. These findings suggest that CD248 activates FAK/Src/JNK/c-Jun, thereby inducing PD-L1 expression on CAFs, thereby promoting immune escape of NSCLC.

To evaluate whether non-small cell lung cancer (NSCLC)-derived CAFs express PD-L1, researchers performed IF staining to detect CD248 and PD-L1 in NSCLC tissues and NATs. Results showed elevated PD-L1 expression in NSCLC samples, with significant colocalization of CD248 and α-SMA (Figure 1A). qPCR analysis revealed significantly higher PD-L1 mRNA levels in CAFs than in NFs (Figure 1B). These results were further validated by Western blotting, which showed increased PD-L1 protein expression in CAFs compared with NFs (Figure 1C). To explore the relationship between CD248 and PD-L1 expression in CAFs, qPCR was performed on CD248-overexpressing CAFs (CAFs-CD248OE), CD248-knockdown CAFs (CAFs-sh-CD248), and control CAFs (CAFs-sh-CON). Compared with the control group, PD-L1 expression was significantly decreased in CD248-knockdown CAFs, whereas expression was increased in CD248-overexpressing CAFs (Figure 1D). Western blot analysis further confirmed that CD248 knockdown reduced PD-L1 protein levels. Concomitantly, CD248 overexpression led to increased PD-L1 expression in CAFs (Figure 1E). IF staining consistently demonstrated a stronger PD-L1 signal in tissues containing CD248-overexpressing CAFs (Figure 1F). These results indicate that CD248+ CAFs upregulate PD-L1 expression.

Figure 1. CD248+CAFs express high levels of PD-L1. (Yang Z, et al., 2025)

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