Panoply™ Human BIRC5 Over-expressing Stable Cell Line

Name: Panoply™ Human BIRC5 Over-expressing Stable Cell Line
SKU: CSC-SC001446
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC001446

Host Cell : HEK293 (CHO and other cell types are also available) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC001446
Description	Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene	BIRC5
Gene Species	Homo sapiens (Human)
Host Cell	HEK293 (CHO and other cell types are also available)
Host Cell Species	Species varies
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	2 × 10^6 cells / vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry Ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	BIRC5 baculoviral IAP repeat containing 5 [ Homo sapiens ]
Gene Symbol	BIRC5
Synonyms	API4; EPR-1
Gene Description	baculoviral IAP repeat-containing 5 (survivin)
Gene ID	332
Uni Prot ID	H3BLT4
m RNA Refseq	NM_001012271.1
Protein Refseq	NP_001012271.1
Chromosome Location	17q25
Function	Ran GTPase binding; chaperone binding; cobalt ion binding; cofactor binding; cysteine-type endopeptidase inhibitor activity; cysteine-type endopeptidase inhibitor activity involved in apoptotic process; enzyme binding; identical protein binding; metal ion binding; microtubule binding; microtubule binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein homodimerization activity; tubulin binding; zinc ion binding; zinc ion binding;
Pathway	Apoptosis, organism-specific biosystem; Apoptosis Modulation and Signaling, organism-specific biosystem; Aurora A signaling, organism-specific biosystem; Aurora B signaling, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Colorectal cancer, organism-specific biosystem;
MIM	603352

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Colorectal cancer (CRC) is one of the most common human malignancies. Alterations in the DNA damage response (DDR) pathway are emerging as novel therapeutic targets for various cancer types, including CRC. RFWD3 plays a key role in replication protein A (RPA)-mediated DNA damage in cancer cells. Importantly, RFWD3 can respond to DNA damage by positively regulating p53 stability during G1 cell cycle checkpoint activation. Here, researchers show that knockdown of RFWD3 inhibits CRC development and progression in vitro and in vivo. Exploring the downstream mechanisms of RFWD3 action, researchers discovered that RFWD3 can transcriptionally activate BIRC5 through interaction with E2F transcription factor 1 (E2F1). Therefore, BIRC5 is a downstream gene of RFWD3 in CRC regulation. Subsequent loss- and gain-of-function experiments demonstrated that overexpression of BIRC5 in RFWD3-depleted RKO cells reversed the inhibitory effects on cell proliferation, migration, and colony formation, while also improving apoptosis, indicating that the effects of RFWD3 depletion are primarily due to BIRC5 inhibition.

To confirm the role of BIRC5 in RFWD3-mediated CRC, the researchers used BIRC5-overexpressing RKO cell, RFWD3-knockdown RKO cell, and RFWD3-knockdown combined with BIRC5-overexpressing RKO cell, and conducted in vitro experiments. Simultaneously, changes in cell phenotype in these RKO cell models were observed using Celigo-based methods, scratch healing/Transwell migration assays, and flow cytometry. Consistent with previous findings, RFWD3 silencing significantly inhibited cell proliferation and migration. Conversely, in BIRC5-overexpressing RKO cells, cell proliferation, migration, and colony formation were enhanced, while apoptosis was reduced. Importantly, overexpressing BIRC5 in RFWD3-knockdown RKO cells reversed the inhibitory effects on cell proliferation, migration, and colony formation, while apoptosis was also improved, indicating that the effects of RFWD3 depletion were primarily due to BIRC5 inhibition (Figures 1A-E).

Figure 1. Overexpression of BIRC5 alleviates the inhibitory effects of RFWD3 knockdown on colorectal cancer cells. (Xu F, et al., 2021)

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