MIR200b-pCMV-MIR

Name: MIR200b-pCMV-MIR
SKU: MIPM2052

For research use only. Not intended for any clinical use.

Cat.No.

MIPM2052

Description

Expression plasmid for mouse microRNA Mir200b. All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA. Both mature and minor (star) miRNA can be over expressed from the construct.

Reporter

GFP

Size/Form

The plasmid is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5 and 3 vector primers in separate vials.

Species

Mouse

Vector Type

plasmid

miRNA Information

Precursor Accession No.

MI0000243

Precursor Name

mmu-mir-200b

Precursors sequence

GCCGUGGCCAUCUUACUGGGCAGCAUUGGAUAGUG UCUGAUCUCUAAUACUGCCUGGUAAUGAUGACGGC

Precursors Length

70 bp

Gene Description

Mus musculus miR-200b stem-loop

Gene Species

Mouse

Mature miRNA-1 Accession No.

MIMAT0004545

Mature miRNA-1 Name

mmu-miR-200b-5p

Mature miRNA-1 sequence

CAUCUUACUGGGCAGCAUUGGA

Mature miRNA-2 Accession No.

MIMAT0000233

Mature miRNA-2 Name

mmu-miR-200b-3p

Mature miRNA-2 sequence

UAAUACUGCCUGGUAAUGAUGA

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