Human TRPV3 Stable Cell Line-HEK293

Name: Human TRPV3 Stable Cell Line-HEK293
SKU: CSC-RI0058
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0058

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0058
Description	This cell line is engineered to overexpress human TRPV3.
Target Gene	TRPV3
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuTRPV3
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TRPV3 transient receptor potential cation channel, subfamily V, member 3 [ Homo sapiens ]
Gene Symbol	TRPV3
Synonyms	OLMS; VRL3
GeneID	162514
Uni ProtID	B2KYM6
mRNA Refseq	NM_001258205.1
Protein Refseq	NP_001245134.1
Chromosome Location	17p13.2
Function	calcium channel activity;
MIM	607066

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The TRPV3 channel belongs to the transient receptor potential channel (TRP) super-family that consists of 7 subfamilies. The channels exist as tetramers where each subunit consists of 6 putative trans-membrane domains. In this respect they have a similar topology to many other voltage-gated channels. The vast majority are permeable to both monovalent cations and calcium. The TRPV3 channel was first cloned in 2002 by two groups based on its homology to known TRPV channels. It shares 40-50% homology to TRPV1 and is activated at temperatures ≥ 34 °C as well as by 2-Aminoethoxydiphenyl borate and monoterpenes such as camphor. In humans it is expressed in skin keratinocytes, trigeminal ganglia, spinal cord and brain. It is also located in DRG neurons where it may even form heteromultimers with TRPV1. The specific distribution of this channel, coupled with its thermal sensitivity and potential activation by a variety of inflammatory mediators, have suggested that it may represent a novel drug target for the treatment of inflammatory pain.

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