Human TRPA1 Stable Cell Line-HEK293

Name: Human TRPA1 Stable Cell Line-HEK293
SKU: CSC-RI0056
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0056

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0056
Description	This cell line is engineered to overexpress human transient receptor potential cation channel subfamily A member 1 (TRPA1).
Target Gene	TRPA1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TRPA1 transient receptor potential cation channel, subfamily A, member 1 [ Homo sapiens ]
Gene Symbol	TRPA1
Synonyms	ANKTM1
Gene Description	transient receptor potential cation channel, subfamily A, member 1
GeneID	277328
Uni ProtID	O75762
mRNA Refseq	NM_007332.2
Protein Refseq	NP_015628.2
Chromosome Location	8q13
Function	calcium channel activity; channel activity;
MIM	604775

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How was the Human TRPA1 Stable Cell Line-HEK293 established, and what special techniques were used?

A: The cell line was established by stably transfecting the human TRPA1 gene (Transient Receptor Potential Cation Channel Subfamily A Member 1) into HEK293 cells using genetic engineering techniques.

What unique advantages does the Human TRPA1 Stable Cell Line-HEK293 offer in studying inflammation and pain mechanisms?

A: Due to the key role of TRPA1 in inflammation and pain perception, this cell line can be used to study the molecular mechanisms of TRPA1 in these processes.

What should be considered when using the Human TRPA1 Stable Cell Line-HEK293 for drug screening?

A: Attention should be paid to the impact of drugs on TRPA1 channel activity, especially when evaluating potential analgesic and anti-inflammatory drugs.

How should overgrowth issues be handled when maintaining the Human TRPA1 Stable Cell Line-HEK293?

A: Regularly change the culture medium, control cell density, and avoid overgrowth that can lead to changes in cell characteristics.

What are the potential uses of the Human TRPA1 Stable Cell Line-HEK293 in dermatology research?

A: It can be used to study the role of TRPA1 in skin inflammatory responses and dermatological conditions, such as contact dermatitis.

What applications does the Human TRPA1 Stable Cell Line-HEK293 have in taste research?

A: Due to the role of TRPA1 in taste perception, this cell line can be used to study the transduction of taste signals and the molecular mechanisms of taste disorders.

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