Cat.No. : CSC-RG0067 Host Cell: CHO-K1
| Cat. No. | CSC-RG0067 |
| Background | This gene encodes a G protein-coupled receptor (GPR) which belongs to the rhodopsin family of GPRs. The encoded protein functions as a receptor for free fatty acids, including omega-3, and participates in suppressing anti-inflammatory responses and insulin sensitizing. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Gene | O3FAR1 |
| Gene Species | Homo sapiens (Human) |
| Alias | O3FAR1, GPR120, GPR129, PGR4, GT01, BMIQ10, MGC119984, DKFZp686F0824 |
| Host Cell | CHO-K1 |
| Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: During the drug screening process, the production efficiency of the Human O3FAR1 Stable Cell Line-CHO can be improved by optimizing culture conditions such as temperature, pH, nutrient concentration, and growth factors. High-throughput screening techniques, combined with automation equipment, can be used to quickly screen for high-producing cell clones. Additionally, gene editing technologies like CRISPR-Cas9 can further optimize the cell line to enhance the expression of specific drug targets or improve cellular metabolic pathways.
A: Quality control for the Human O3FAR1 Stable Cell Line-CHO cell line during the production process involves multiple aspects. Firstly, ensuring the genetic stability of the cell line is crucial, which is monitored through regular genetic testing. Secondly, precise control of bioreactor conditions such as temperature, pH, dissolved oxygen, and nutrient supply is necessary. Regular protein quality analysis, including glycosylation analysis, purity, and activity tests, is also performed to ensure the consistency and quality of the final product. Lastly, adhering to GMP (Good Manufacturing Practice) standards ensures compliance and product quality throughout the production process.
A: The Human O3FAR1 Stable Cell Line-CHO cell line plays a key role in vaccine development. This cell line can be used to produce vaccine antigens, especially those that require complex glycosylation patterns. The ability of CHO cell lines to mimic the glycosylation process of human cells is crucial for the immunogenicity and safety of vaccines. Additionally, the high productivity and ease of genetic manipulation of this cell line make it an ideal choice for large-scale vaccine production.
A: To minimize genotoxic risks when genetically engineering the Human O3FAR1 Stable Cell Line-CHO cell line, several strategies should be employed. First, using non-integrative vectors, such as plasmids or viral vectors, can reduce the direct insertion into the cell genome, thus lowering genotoxicity. Second, optimizing transfection conditions to ensure high transfection efficiency and cell viability can reduce potential damage to the cells. Additionally, the use of selective markers, such as antibiotic resistance genes, should be carefully chosen to avoid adverse effects on cell function. Finally, rigorous safety assessments of the engineered cell lines should be conducted, including monitoring genomic stability, cell growth characteristics, and protein expression levels.
A: To optimize post-translational modifications (PTMs) of recombinant proteins produced by the Human O3FAR1 Stable Cell Line-CHO cell line, several strategies can be employed. First, adjusting the culture medium composition, such as adding specific amino acids, vitamins, and trace elements, can influence the glycosylation pattern of proteins. Second, altering culture conditions, such as temperature and pH, can further optimize PTMs. Additionally, using specific bioreactor designs, like agitation rate and oxygen supply, can help control PTMs. Finally, genetic engineering approaches, such as knocking out or overexpressing specific enzymes, can be used to directly regulate PTM pathways within the cells.
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