Human O3FAR1 Stable Cell Line-HEK293

Name: Human O3FAR1 Stable Cell Line-HEK293
SKU: CSC-RG0857
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0857

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0857
Background	GPR120 is a member of the free fatty acid G protein-coupled receptor family that includes GPR40, GPR41 and GPR43, and is activated by unsaturated long chain free fatty acids (Hirasawa et al., 2005). Expression analysis shows that GPR120 is abundantly expressed in intestine. Stimulation of GPR120 by free fatty acids has been shown to promote the secretion of glucagon-like peptide-1 (GLP-1) and activate the ERK cascade (Hirasawa et al., 2005). More recently, Katsuma et al showed that activation of GPR120 by free fatty acids such as linolenic acid inhibits apoptosis of a murine enteroendocrine cell line.
Target Gene	O3FAR1
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuO3FAR1
Alias	O3FAR1, GPR120, GPR129, PGR4, GT01, BMIQ10, MGC119984, DKFZp686F0824
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	O3FAR1 omega-3 fatty acid receptor 1 [ Homo sapiens ]
Gene Symbol	O3FAR1
Synonyms	GT01; PGR4; BMIQ10; GPR120; GPR129
Gene ID	338557
Uni Prot ID	B4DWG6
m RNA Refseq	BC101175
Chromosome Location	10q23.33
Function	fatty acid binding; taste receptor activity;
Pathway	Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Free fatty acid receptors, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Incretin Synthesis, Secretion, and Inactivation, organism-specific biosystem; Integration of energy metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Regulation of Insulin Secretion, organism-specific biosystem;
MIM	609044

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