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Cat.No. : CSC-RG0857 Host Cell: HEK293
| Cat. No. | CSC-RG0857 |
| Background | GPR120 is a member of the free fatty acid G protein-coupled receptor family that includes GPR40, GPR41 and GPR43, and is activated by unsaturated long chain free fatty acids (Hirasawa et al., 2005). Expression analysis shows that GPR120 is abundantly expressed in intestine. Stimulation of GPR120 by free fatty acids has been shown to promote the secretion of glucagon-like peptide-1 (GLP-1) and activate the ERK cascade (Hirasawa et al., 2005). More recently, Katsuma et al showed that activation of GPR120 by free fatty acids such as linolenic acid inhibits apoptosis of a murine enteroendocrine cell line. |
| Gene | O3FAR1 |
| Gene Species | Homo sapiens (Human) |
| Alias | O3FAR1, GPR120, GPR129, PGR4, GT01, BMIQ10, MGC119984, DKFZp686F0824 |
| Host Cell | HEK293 |
| Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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