Human NR3C1 Prom/Luciferase/Hyg Reporter Stable Cell Line-HEK293

Name: Human NR3C1 Prom/Luciferase/Hyg Reporter Stable Cell Line-HEK293
SKU: CSC-RR0027
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0027

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RR0027
Target Gene	NR3C1
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuNR3C1 Prom/Luciferase/Hyg
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	NR3C1 nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) [ Homo sapiens ]
Gene Symbol	NR3C1
Synonyms	GR; GCR; GRL; GCCR
Gene Description	nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
GeneID	2908
Uni ProtID	E5KQF6
mRNA Refseq	NM_001024094.1
Protein Refseq	NP_001019265.1
Chromosome Location	5q31.3
Function	Hsp70 protein binding; Hsp90 protein binding; chromatin binding; double-stranded DNA binding; glucocorticoid receptor activity; protein binding; protein heterodimerization activity; protein homodimerization activity; receptor tyrosine kinase binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; steroid binding; zinc ion binding;
Pathway	Adipogenesis, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BMAL1:CLOCK/NPAS2 Activates Circadian Expression, organism-specific biosystem; Circadian Clock, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem;
MIM	138040

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Glucocorticoids, a major subclass of steroid hormones, modulate a large number of metabolic, cardiovascular, immune, and behavioral functions. The intracellular effects of glucocorticoids are mediated by the glucocorticoid receptor (GR), a 94-kDa intracellular protein belonging to the phylogenetically conserved nuclear hormone receptor superfamily. In the absence of glucocorticoid, the GR is maintained predominantly in the cytoplasm as part of an inactive multiprotein complex that consists of the receptor, two Hsp90 molecules, one molecule each of Hsp70 and Hsp56, and an immunophilin of the FK506- and rapamycin-binding class. When glucocorticoid binds to GR, the receptor undergoes a change in conformation, dissociates from regulatory heat shock proteins, and is hyperphosphorylated. The activated receptor rapidly translocates to the cell nucleus, where it is able to initiate transcriptional regulation.

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