Human KCNN1 Stable Cell Line-CHO-K1

Name: Human KCNN1 Stable Cell Line-CHO-K1
SKU: CSC-RI0107
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0107

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0107
Description	This cell line is engineered to overexpress human KCNN1.
Background	The KCNN1 gene encodes the pore-forming subunit of a small-conductance (2 - 3 pS single channel conductance), K+-selective, channel that is activated by increased intracellular [Ca2+]. Expressed in neurons, hKCa2.1 channels contribute to the afterhyperpolarization that modulates repetitive firing frequency.
Target Gene	KCNN1
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuKCNN1
Alias	KCNN1, hSK1, KCa2.1, SKCa 1, SK1, SKCA1
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KCNN1 potassium intermediate/small conductance calcium-activated channel, subfamily N, member 1 [ Homo sapiens ]
Gene Symbol	KCNN1
Synonyms	SK1; hSK1; SKCA1; KCa2.1
Gene Description	potassium intermediate/small conductance calcium-activated channel, subfamily N, member 1
GeneID	3780
Uni ProtID	Q92952
mRNA Refseq	NM_002248.3
Protein Refseq	NP_002239.2
Chromosome Location	19p13.1
Function	calcium-activated potassium channel activity; calmodulin binding; small conductance calcium-activated potassium channel activity;
Pathway	Ca2+ activated K+ channels, organism-specific biosystem; Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem;
MIM	602982

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Customer Reviews

Customer Q&As

What unique insights can this cell line provide in cell signaling research?

A: It can provide information on the role of KCNN1 in intracellular calcium signaling and electrical signal regulation, which is crucial for understanding signaling pathways.

What aspects should be focused on when using the Human KCNN1 Stable Cell Line-CHO to study cell metabolism?

A: Focus on the role of KCNN1 in regulating intracellular potassium concentration and its impact on the metabolic state of the cell.

What is the potential application of the Human KCNN1 Stable Cell Line-CHO in researching neurodegenerative diseases?

A: Given the role of KCNN1 in neuronal cell function, this cell line can be used to study the molecular mechanisms of diseases like Alzheimer’s and Parkinson’s.

What are the advantages of using this cell line in ion channel kinetics research?

A: It provides a stable model expressing a specific potassium channel, helpful for in-depth study of channel opening and closing kinetics.

How to maximize the use of the Human KCNN1 Stable Cell Line-CHO in drug screening?

A: Utilize the potassium channel activity of this cell line for high-throughput screening by precisely controlling the concentration and exposure time of drugs.

What types of pharmacological research is the Human KCNN1 Stable Cell Line-CHO particularly suited for?

A: It is suitable for studying modulators of the small conductance calcium-activated potassium channel (KCNN1), especially in the treatment of neurological and cardiovascular diseases.

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