Human KCNH3 Stable Cell Line-HEK293

Name: Human KCNH3 Stable Cell Line-HEK293
SKU: CSC-RI0034
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0034

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0034
Description	This cell line is engineered to overexpress human KCNH3.
Target Gene	KCNH3
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuKCNH3
Alias	KCNH3, BEC1, elk2, Kv12.2, ELK2, KIAA1282
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KCNH3 potassium voltage-gated channel, subfamily H (eag-related), member 3 [ Homo sapiens ]
Gene Symbol	KCNH3
Synonyms	BEC1; ELK2; Kv12.2
Gene Description	potassium voltage-gated channel, subfamily H (eag-related), member 3
GeneID	23416
Uni ProtID	Q9ULD8
mRNA Refseq	NM_012284.1
Protein Refseq	NP_036416.1
Chromosome Location	12q13
Function	two-component sensor activity; voltage-gated potassium channel activity;
Pathway	Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem; Voltage gated Potassium channels, organism-specific biosystem;
MIM	604527

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The human KCNH3 gene encodes for the pore-forming subunit of the subfamily H (eag-related) voltage-gated potassium channel #3, a protein known as the Kv12.2 potassium ion channel. The gene was cloned & first described as BEC1 by Miyake et al. 1999. Gene deletion experiments in mice showed KCNH3 knock-out mice performed better in working memory, reference memory, and attention-related behavioral assays than their wild-type littermates; enhanced performance was also observed in heterozygous mutants. Interestingly, when KCNH3 was overexpressed in the forebrain impaired performance was observed in the behavioral assays. Altering KCNH3 expression changed hippocampal neuronal excitability and synaptic plasticity; suggesting that Kv12.2 could be an interesting target for cognitive enhancement or perhaps epilepsy.

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