Human HNF4A adenoviral particles

Name: Human HNF4A adenoviral particles
SKU: AD07632Z

Cat.No. : AD07632Z

Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL

Storage: -80℃ Shipping: Frozen on dry ice

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Adenovirus Particle Information

Quality Control

Cat. No.	AD07632Z
Description	Adenovirus with ORF of hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 2.
Target Gene	HNF4A
Species	Human
Titer	Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc.
Size	Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance.
Sterility	Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination.
Ad5 E1 Detection	All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination.
RCA Assays	Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources.
PFU Titering	All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells.

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The transcription factor hepatocyte nuclear factor 4α (HNF4A) is critical for maintaining epithelial differentiation and mature function in renal proximal tubules (PTs). The researchers investigated HNF4A dynamics during acute and chronic human kidney disease and in cultured primary proximal tubular epithelial cells (PTECs). Immunohistochemical analyses revealed a reciprocal relationship between the loss of HNF4A and the gain of vimentin expression in injured kidneys, reflecting PT dedifferentiation. In vitro, primary PTECs rapidly downregulated HNF4A and proximal tubular markers while upregulating vimentin during subculture. Adenoviral-mediated re-expression of HNF4A using Creative Biogene's human HNF4A adenovirus restored transcripts associated with brush border formation and absorptive/transport functions, as confirmed by RNA sequencing and gene set enrichment analyses, providing a robust model to study PT injury and regeneration mechanisms.

Figure 1. Immunohistochemistry and in vitro culture demonstrated that HNF4A expression declines while vimentin levels increase in primary PTECs, reflecting PT dedifferentiation during kidney injury and cell culture. (Kha M, et al., 2025)

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