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Panoply™ Human HNF4A Over-expressing Stable Cell Line

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-SC007146

Host Cell :   HEK293 (CHO and other cell types are also available) Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-SC007146
Description Using Creative Biogene's proprietary lentiviral vectors, we subclone the target gene into lentivector, generate the lentivirus particles, sequentially infect the cell line HEK293 (other cell types are also available according to your requirements), and select the clones constantly expressing target gene at high level.
Target Gene HNF4A
Gene Species Homo sapiens (Human)
Host Cell HEK293 (CHO and other cell types are also available)
Host Cell Species Species varies
Applications

1. Gene expression studies

2. Signaling pathway research

3. Drug screening and toxicology

4. Disease research

Size 2 × 10^6 cells / vial
Stability Validated for at least 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry Ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name HNF4A hepatocyte nuclear factor 4, alpha [ Homo sapiens ]
Gene Symbol HNF4A
Synonyms TCF; HNF4; MODY; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
GeneID 3172
Uni ProtID F1D8T1
mRNA Refseq NM_000457.4
Protein Refseq NP_000448.3
Chromosome Location 20q13.12
Function DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; activating transcription factor binding; fatty acid binding; fatty-acyl-CoA binding; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; palmitoyl-CoA hydrolase activity; protein binding; protein homodimerization activity; receptor binding; repressing transcription factor binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; steroid hormone receptor activity; transcription regulatory region DNA binding; zinc ion binding;
Pathway AMPK signaling, organism-specific biosystem; Developmental Biology, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Gene Expression, organism-specific biosystem; Generic Transcription Pathway, organism-specific biosystem; HIF-1-alpha transcription factor network, organism-specific biosystem; Integrated Pancreatic Cancer Pathway, organism-specific biosystem;
MIM 600281
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