Human FEN1 Knockdown Cell Line-HeLa

Name: Human FEN1 Knockdown Cell Line-HeLa
SKU: CSC-RK0270
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RK0270

Host Cell : HeLa Validation : Real-Time RCR

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Cell Line Information

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Gene Information

Cat. No.	CSC-RK0270
Target Gene	FEN1
Abbr	HeLa-HuFEN1 Knockdown
Alias	FEN1, RAD2, DNase IV, FEN 1, MF1, FEN-1
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Applications	(1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models
Morphology	Epithelial
Size	>1 × 10⁶ cells / vial
Stability	Validated for at least 10 passages
Validation	Real-Time RCR
Shipping	Dry ice

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	FEN1 flap structure-specific endonuclease 1 [ Homo sapiens ]
Gene Symbol	FEN1
Synonyms	FEN1; flap structure-specific endonuclease 1; RAD2; flap endonuclease 1; DNase IV; FEN 1; maturation factor 1; MF1; maturation factor-1; FEN-1;
GeneID	2237
Uni ProtID	P39748
mRNA Refseq	BC000323
Chromosome Location	11q12
Function	5-3 exonuclease activity; 5-flap endonuclease activity; 5-flap endonuclease activity; DNA binding; damaged DNA binding; double-stranded DNA binding; double-stranded DNA specific exodeoxyribonuclease activity; endonuclease activity; exonuclease activity; magnesium ion binding; manganese ion binding; protein binding; ribonuclease H activity;
Pathway	Base Excision Repair, organism-specific biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; DNA Repair, organism-specific biosystem;
MIM	600393

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The protein encoded by this gene removes 5" overhanging flaps in DNA repair and processes the 5" ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5" end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

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