The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3" of the DNA lesion while the ERCC1-XPF complex makes the 5" incision. Elevated levels of ERCC1 have also been reported in Cisplatin-resistant cells.
DNA Repair, organism-specific biosystem; Dual incision reaction in GG-NER, organism-specific biosystem; Dual incision reaction in TC-NER, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; Formation of incision complex in GG-NER, organism-specific biosystem; Formation of transcription-coupled NER (TC-NER) repair complex, organism-specific biosystem;
damaged DNA binding; damaged DNA binding; endonuclease activity; hydrolase activity; protein C-terminus binding; protein binding; protein domain specific binding; single-stranded DNA binding; contributes_to single-stranded DNA specific endodeoxyribonuclease activity; contributes_to structure-specific DNA binding;