Cat.No. : CSC-RR0475 Host Cell: HEK293
| Cat. No. | CSC-RR0475 |
| Description | SRE-Luc Reporter Cell Line-HEK293 stably expresses luciferase reporter gene under the control of the serum response element (SRE). This cell line is an ideal cellular model for monitoring GPCR-linked MAPK/ERK signaling pathways. |
| Gene | SRE-Luc |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
In the context of serum response element (SRE), CREB-binding protein (CBP) can interact with transcription factors such as serum response factor (SRF) to regulate gene expression. After activating signaling pathways that converge on SREs, SRF binds to SRE sequences in the promoter region of target genes. CBP then interacts with SRF to enhance its transcriptional activity, facilitating the recruitment of additional coactivators and components of the transcriptional machinery to promote gene transcription.
This interaction between CBP and SRF at SREs helps coordinate cellular responses to extracellular stimuli, particularly those associated with growth factors and cellular stress. By integrating signals from multiple pathways, CBP helps to precisely regulate gene expression essential for a variety of cellular processes, including cell proliferation, differentiation, and survival.
Figure 1. CBP stimulates SRE activity. In the absence of CBP expression, the SRE-luc reporter vector expressed low levels and was less active. Concomitant expression of CBP increased basal expression levels and enhanced responses to serum. The response depended on the dose of the CBP expression vector. CBP did not stimulate transcription in general, since no increase in the level of luciferase was observed with an enhancerless reporter vector containing only the c-fos minimal promoter. This result suggests that CBP participates in transactivation through the c-fos SRE. (Ramirez S, et al., 1997)
A: The SRE-Luc reporter gene is a combination of the serum response element (SRE) and the luciferase gene. It is used to study the activation of the serum response element, which is a DNA sequence that responds to signals such as growth factors and cytokines. The luciferase gene allows for the measurement of SRE activity by producing light when the SRE is activated.
A: The SRE-Luc reporter gene helps in studying gene regulation by providing a sensitive and quantitative readout of the activation of the SRE. By monitoring the light output from the luciferase enzyme, researchers can determine how signals such as growth factors and cytokines affect the transcriptional activity of genes that contain the SRE in their promoters.
A: The SRE-Luc reporter system offers several advantages, including its sensitivity, ease of use, and ability to measure gene expression in real time. It also allows for high-throughput screening of compounds or conditions that affect SRE-dependent gene expression.
A: While the SRE-Luc reporter system is a useful tool, it does have limitations. It measures SRE-dependent gene expression but does not provide information on other regulatory elements or post-translational modifications. Additionally, the luciferase enzyme requires the addition of a substrate to produce light, which can be a limitation in certain experimental settings.
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The SRE-Luc Reporter Cell Line-HEK293 demonstrates efficient transduction capabilities, allowing for robust and reliable monitoring of SRE-mediated gene expression, which is essential for studying signaling pathways and nuclear receptor activity.
This cell line offers excellent value for money, providing researchers with a cost-effective solution for their experimental needs without compromising on quality or performance, making it an attractive option for budget-conscious laboratories.
The SRE-Luc Reporter Cell Line-HEK293 is supplied by a reputable and trusted source, ensuring the reliability and consistency of the product, and giving researchers confidence in the quality and authenticity of the cell line.
The SRE-Luc Reporter Cell Line-HEK293 is produced under standardized conditions, ensuring that each batch of cells is uniform and consistent, allowing for reproducible results across different experiments and facilitating comparison of data between different research groups.
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