scAAV3-Cre

Name: scAAV3-Cre
SKU: AAV00173Z
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : AAV00173Z

Serotype : AAV Serotype 3 Storage : -80 ℃

Titer: Size:

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Cat. No.	AAV00173Z
Description	Self-complementary AAV serotype 3 particles contain Cre recombinase under the control of CMV promoter.
Serotype	AAV Serotype 3
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 100 μL, 500 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Summary	Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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Adeno-associated virus (AAV) vectors are widely used to stably transfer genes into terminally differentiated or quiescent cells, such as myofibers, hepatocytes, neurons, retinal cells, etc. These vectors, derived from a non-pathogenic, replication-defective parvovirus with a small single-stranded (ss) DNA genome, have recently been successfully used for clinical gene transfer for inherited blindness and also show promise for the treatment of other diseases.

In recent years, many alterations of the capsid and vector genome have been developed in an attempt to improve gene transfer efficiency and potentially evade immunity. Modifications of the recombinant AAV genome can also improve transduction rates. As ss, the ssAAV genome must be converted to a double-stranded form in the nucleus of infected cells for transgene expression. To overcome this rate-limiting step, self-complementary (sc)AAV vectors were developed by eliminating the terminal cleavage site in one of the inverted terminal repeats (ITRs). In order to package such a genome into the capsid, the size of the expression cassette must be further reduced to not exceed the packaging limit.

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The scAAV3-Cre product offers a perfect balance between quality and cost, making it an excellent choice for labs with budget constraints. Despite its competitive pricing, the quality of the viral vectors is top-notch, with high titers and purity ensured for every batch.

United States

2022-08-19

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scAAV3-Cre

Virus Particles Information

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