Cat.No. : CSC-SC004310-RH Host Cell: HEK293
| Cat. No. | CSC-SC004310-RH |
| Description | This cell line is engineered to stably overexpress rat iodothyronine deiodinase 3 (Dio3, also known as 5DIII, DIOIII). |
| Gene | Dio3 |
| Host Cell | HEK293 |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Deiodinase enzymes are vital for regulating the activation and inactivation of thyroid hormones, playing a key role in vertebrate development, including amphibian metamorphosis. Researchers screened various chemicals for their inhibitory effects on the human Type 3 iodothyronine deiodinase enzyme (hDIO3) and produced a recombinant version from Xenopus laevis (Xldio3). They found that many tested compounds had lower inhibitory potency against Xldio3 compared to hDIO3, suggesting cross-species differences. However, a subset of eight chemicals exhibited strong specificity, with IC50 values indicating effective inhibition. This study underscores the predictive value of human assays for amphibian enzyme activity and highlights the importance of recombinant non-mammalian proteins in evaluating chemical sensitivities.
Figure 1. The researchers optimized enzyme assays by transfecting HEK293 cells with plasmids to compare the activity of X. laevis and human DIO3 under various conditions. (Mayasich SA, et al., 2021)
Creative Biogene's Rat Dio3 Stable Cell Line - HEK293 offers researchers a powerful tool for investigating deiodinase functions and the biochemical pathways regulated by thyroid hormones. This cell line is designed for high expression of Dio3, making it suitable for screening potential inhibitors and exploring their effects in a controlled environment.
A: The Dio gene encodes D-isovaleryl-CoA dehydrogenase, which is involved in specific amino acids and fatty acids. This enzyme is present in mitochondria β The oxidation pathway plays a crucial role in converting D-isovaleryl-CoA to 3-methylcrotonyl CoA.
A: The Dio gene contributes to energy production by participating in the metabolism of branched-chain fatty acids and certain amino acids. The D-isovaleryl-CoA dehydrogenase enzyme helps generate acetyl-CoA, which can enter the citric acid cycle (TCA cycle) to produce ATP, the cell's energy currency.
A: Yes, mutations in the Dio gene can lead to D-isovaleryl-CoA dehydrogenase deficiency, a rare metabolic disorder. This condition can result in the accumulation of toxic metabolites, leading to symptoms such as hypoglycemia, lethargy, vomiting, and potentially life-threatening metabolic crises.
A: The expression of the Dio gene is regulated in response to metabolic demands and the availability of substrates. Transcription factors and signaling pathways involved in mitochondrial metabolism, such as the PPARα pathway, can influence the expression of Dio. This regulation ensures that the D-isovaleryl-CoA dehydrogenase enzyme is produced when needed for the breakdown of specific substrates.
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The Rat Dio3 Stable Cell Line - HEK293 is created using an advanced gene editing platform, offering high precision and efficiency in targeting the Dio3 gene. This cutting-edge technology ensures the reliable and consistent generation of the desired genetic modifications, providing researchers with a robust tool for investigating the functions of the Dio3 gene.
This cell line offers a variety of editing strategies, allowing researchers to choose the most suitable approach for their specific research objectives. Whether it's gene knockout, knock-in, or other sophisticated modifications, the Rat Dio3 Stable Cell Line - HEK293 provides researchers with the flexibility to tailor their studies to their needs.
The gene editing technology used for the Rat Dio3 Stable Cell Line - HEK293 minimizes off-target effects, ensuring high specificity in the editing process. This reduces the likelihood of unintended changes to other genes, providing researchers with confidence in the accuracy and reliability of their experimental results.
The Rat Dio3 Stable Cell Line - HEK293 is well-characterized, with detailed background information available to researchers. This includes information about the cell line's origin, genetic modifications, and any known characteristics, allowing researchers to make informed decisions and accurately interpret their experimental findings.
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