Rat Dio3 Stable Cell Line - HEK293

Name: Rat Dio3 Stable Cell Line - HEK293
SKU: CSC-SC004310-RH
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC004310-RH

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC004310-RH
Description	This cell line is engineered to stably overexpress rat iodothyronine deiodinase 3 (Dio3, also known as 5DIII, DIOIII).
Target Gene	Dio3
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Dio3 deiodinase, iodothyronine type III [ Mus musculus ]
Gene Symbol	Dio3
Gene Description	deiodinase, iodothyronine type III
Gene ID	107585
Uni Prot ID	Q91ZI8
m RNA Refseq	NM_172119.2
Protein Refseq	NP_742117.2
Chromosome Location	12; 12 F1
Function	oxidoreductase activity; thyroxine 5-deiodinase activity; thyroxine 5-deiodinase activity;
Pathway	Selenium metabolism/Selenoproteins, organism-specific biosystem; thyroid hormone metabolism I (via deiodination), organism-specific biosystem;

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Background

Case Study

Applications

Q & A

Customer Reviews

The DIO3 gene, which stands for Deiodinase 3, is an enzyme responsible for the conversion of thyroxine (T4) to triiodothyronine (T3), the more active form of thyroid hormone. This gene plays a crucial role in the regulation of thyroid hormone levels in the body and is involved in various physiological processes, including growth, development, and metabolism.

The DIO3 gene is primarily expressed in the thyroid gland, kidney, and brain, and its activity is essential for the proper functioning of these organs. It has been found that the DIO3 gene is involved in the adaptation to environmental stress, such as fasting and cold exposure, by regulating thyroid hormone levels.

Abnormal expression or mutations in the DIO3 gene can lead to altered thyroid hormone levels, which may result in various health issues. For example, decreased expression of the DIO3 gene has been associated with thyroid cancer, while increased expression has been linked to conditions such as depression and cardiovascular diseases.

Furthermore, recent studies have suggested that the DIO3 gene may also play a role in the development of neurodegenerative diseases, such as Alzheimer’s and Parkinson’s diseases. It has been observed that the DIO3 gene is involved in the regulation of neurotrophic factors and the protection against neuronal damage.

The DIO3 gene is an important enzyme involved in the regulation of thyroid hormone levels and plays a crucial role in various physiological processes. Abnormal expression or mutations in this gene can lead to diverse health issues, highlighting the importance of maintaining proper thyroid hormone balance in the body. Further research into the function and regulation of the DIO3 gene may contribute to the development of new therapeutic strategies for the treatment of these conditions.

Deiodinase enzymes are vital for regulating the activation and inactivation of thyroid hormones, playing a key role in vertebrate development, including amphibian metamorphosis. Researchers screened various chemicals for their inhibitory effects on the human Type 3 iodothyronine deiodinase enzyme (hDIO3) and produced a recombinant version from Xenopus laevis (Xldio3). They found that many tested compounds had lower inhibitory potency against Xldio3 compared to hDIO3, suggesting cross-species differences. However, a subset of eight chemicals exhibited strong specificity, with IC50 values indicating effective inhibition. This study underscores the predictive value of human assays for amphibian enzyme activity and highlights the importance of recombinant non-mammalian proteins in evaluating chemical sensitivities.

Figure 1 summarizes the optimal assay conditions for measuring the activity of recombinant dio3 enzymes from Xenopus laevis and human DIO3, detailing the effects of temperature, cofactor concentration, pH, and substrate preference on enzyme performance, based on triplicate assays conducted in HEK293 cells. (doi: 10.1016/j.tiv.2021.105141) Figure 1. The researchers optimized enzyme assays by transfecting HEK293 cells with plasmids to compare the activity of X. laevis and human DIO3 under various conditions. (Mayasich SA, et al., 2021)

Creative Biogene's Rat Dio3 Stable Cell Line - HEK293 offers researchers a powerful tool for investigating deiodinase functions and the biochemical pathways regulated by thyroid hormones. This cell line is designed for high expression of Dio3, making it suitable for screening potential inhibitors and exploring their effects in a controlled environment.

The Rat Dio3 Stable HEK293 Cell Line, which overexpresses the Dio3 (Dioxygenase 3) gene, provides a valuable tool for researchers to explore the functions and mechanisms of this enzyme in cellular processes. One of the key applications of this stable cell line is in the study of Dio3’s role in gene regulation and its potential implications in various diseases.

By manipulating Dio3 expression and activity in these cells, researchers can investigate the effects of Dio3 overexpression on gene expression patterns and cellular functions. This can provide insights into the regulatory mechanisms of Dio3 in gene expression and its potential roles in development, aging, and disease.

Furthermore, the Rat Dio3 Stable HEK293 Cell Line can be utilized to study the role of Dio3 in drug resistance and to identify potential therapeutic strategies to overcome this resistance. By testing various compounds and drugs on these cells, researchers can explore the potential targets and mechanisms of action for new anticancer agents.

In summary, the Rat Dio3 Stable HEK293 Cell Line has diverse applications in research, offering valuable insights into the role of Dio3 in gene regulation and potential therapeutic strategies for various diseases. Its utility in drug resistance research also makes it a significant resource for the development of novel treatment approaches.

Customer Q&As

What is the Dio gene and its role in biological processes?

A: The Dio gene encodes D-isovaleryl-CoA dehydrogenase, which is involved in specific amino acids and fatty acids. This enzyme is present in mitochondria β The oxidation pathway plays a crucial role in converting D-isovaleryl-CoA to 3-methylcrotonyl CoA.

How does the Dio gene contribute to energy production?

A: The Dio gene contributes to energy production by participating in the metabolism of branched-chain fatty acids and certain amino acids. The D-isovaleryl-CoA dehydrogenase enzyme helps generate acetyl-CoA, which can enter the citric acid cycle (TCA cycle) to produce ATP, the cell's energy currency.

Are there any diseases associated with mutations in the Dio gene?

A: Yes, mutations in the Dio gene can lead to D-isovaleryl-CoA dehydrogenase deficiency, a rare metabolic disorder. This condition can result in the accumulation of toxic metabolites, leading to symptoms such as hypoglycemia, lethargy, vomiting, and potentially life-threatening metabolic crises.

How is the expression of the Dio gene regulated in response to metabolic demands?

A: The expression of the Dio gene is regulated in response to metabolic demands and the availability of substrates. Transcription factors and signaling pathways involved in mitochondrial metabolism, such as the PPARα pathway, can influence the expression of Dio. This regulation ensures that the D-isovaleryl-CoA dehydrogenase enzyme is produced when needed for the breakdown of specific substrates.

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Customer Reviews

Technological Platform

The Rat Dio3 Stable Cell Line - HEK293 is created using an advanced gene editing platform, offering high precision and efficiency in targeting the Dio3 gene. This cutting-edge technology ensures the reliable and consistent generation of the desired genetic modifications, providing researchers with a robust tool for investigating the functions of the Dio3 gene.

United Kingdom

2022-12-03

Editing Strategy Diversity

This cell line offers a variety of editing strategies, allowing researchers to choose the most suitable approach for their specific research objectives. Whether it's gene knockout, knock-in, or other sophisticated modifications, the Rat Dio3 Stable Cell Line - HEK293 provides researchers with the flexibility to tailor their studies to their needs.

French

2022-05-14

Off-target Effects

The gene editing technology used for the Rat Dio3 Stable Cell Line - HEK293 minimizes off-target effects, ensuring high specificity in the editing process. This reduces the likelihood of unintended changes to other genes, providing researchers with confidence in the accuracy and reliability of their experimental results.

United States

2020-07-01

Background Information

The Rat Dio3 Stable Cell Line - HEK293 is well-characterized, with detailed background information available to researchers. This includes information about the cell line's origin, genetic modifications, and any known characteristics, allowing researchers to make informed decisions and accurately interpret their experimental findings.

United Kingdom

2020-11-21

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Rat Dio3 Stable Cell Line - HEK293

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Case Study

Applications

Q & A

Customer Reviews

What is the Dio gene and its role in biological processes?

How does the Dio gene contribute to energy production?

Are there any diseases associated with mutations in the Dio gene?

How is the expression of the Dio gene regulated in response to metabolic demands?

Technological Platform

Editing Strategy Diversity

Off-target Effects

Background Information

Related Products

Related Services