Cat.No. : CSC-RO0678 Host Cell: HEK293
| Cat. No. | CSC-RO0678 |
| Description | This cell line is engineered to stably co-express mouse Slc7a9 [solute carrier family 7 (cationic amino acid transporter, y+ system), member 9 ] and mouse Slc3a1 [solute carrier family 3, member 1]. |
| Gene | Slc7a9/Slc3a1 |
| Gene Species | Mus musculus (Mouse) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Leucine-rich repeat kinase 2 (LRRK2) has been linked to dopaminergic neuronal vulnerability to oxidative stress (OS), mitochondrial impairment, and increased cell death in both idiopathic and familial Parkinson's disease (PD). However, the exact mechanism by which LRRK2 contributes to these processes remains unclear. The researchers utilized CRISPR/Cas9 technology to generate LRRK2 knockout (KO) HEK-293 cells and analyzed their response to the mitochondrial inhibitor rotenone (ROT), a known inducer of OS and cell death. By comparing LRRK2 wild-type (WT) and KO cells, they found that LRRK2 KO cells showed resistance to ROT-induced OS and apoptosis, suggesting that LRRK2 plays a key role in regulating mitochondrial dysfunction and apoptosis in response to oxidative stress. This observation supports the hypothesis that LRRK2 is a pro-apoptotic kinase involved in PD pathogenesis.
Figure 1. The researchers used cryo-electron microscopy (cryo-EM) to determine the structure of the b(0,+)AT1-rBAT complex. They revealed a heterotetrameric assembly of two rBAT and b(0,+)AT1 subunits and mapped key structural features, including glycosylation sites, disulfide bonds, and subunit interactions, providing insights into the transport mechanism. (Wu D, et al., 2020)
Creative Biogene's Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 is an ideal tool for studying similar pathways related to amino acid transporters and their role in disease mechanisms. The cell line is engineered to express both the SLC3A1 and SLC7A9 genes, enabling research on the b(0,+)AT1-rBAT complex involved in L-arginine transport.
A: The Slc7a9 gene encodes for a subunit of the cystine/glutamate exchange transporter, while Slc3a1 encodes for a subunit of the histidine/histamine transport system. In the Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293, these genes work together to facilitate the transport of amino acids across the cell membrane, which is crucial for cellular metabolism and protein synthesis.
A: By introducing specific mutations into the Slc7a9 and Slc3a1 genes within the cell line, researchers can investigate how these genetic changes affect the efficiency and regulation of amino acid transport. This can provide insights into the molecular basis of transport-related diseases and potential therapeutic targets.
A: The cell line can be used in drug screening assays to identify compounds that modulate the activity of the amino acid transporters encoded by Slc7a9 and Slc3a1. By assessing the effects of these compounds on transporter function, researchers can optimize potential therapeutic agents for the treatment of cystinuria and other related disorders.
A: The cell line provides a controlled environment to study the role of amino acid transporters in various biological processes. By observing how changes in transporter function affect protein synthesis and signaling pathways, researchers can gain a deeper understanding of the complex interplay between these processes and their implications for cellular health.
A: In systems biology, the cell line can be used to construct networks of molecular interactions involving amino acid transporters. This can help researchers identify key regulatory nodes and potential points of intervention for modulating cellular processes related to amino acid transport.
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The Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 is engineered to ensure high-efficiency expression of Slc7a9 and Slc3a1 genes in HEK293 cells.
This product maintains good genetic stability through passaging, ensuring consistency and reproducibility for long-term experiments.
The built-in selection marker in Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 makes the selection of stable expressing cells straightforward.
We can efficiently perform functional analysis of Slc7a9/Slc3a1 with this product to explore its role in cellular physiology.
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