Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293

Name: Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293
SKU: CSC-RO0678
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0678

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

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Cat. No.	CSC-RO0678
Description	This cell line is engineered to stably co-express mouse Slc7a9 [solute carrier family 7 (cationic amino acid transporter, y+ system), member 9 ] and mouse Slc3a1 [solute carrier family 3, member 1].
Target Gene	Slc7a9/Slc3a1
Gene Species	Mus musculus (Mouse)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

CSC-RO0678-Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293-Datasheet

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The Mouse Slc7a9/Slc3a1 Stable Cell Line is engineered to express both the Slc7a9 (xCT) and Slc3a1 (4F2hc) genes, which are responsible for the transport of cystine and glutamate, respectively. These transporters are part of the cystine-glutamate antiporter system, playing a crucial role in the regulation of intracellular redox balance and the synthesis of glutathione, an important antioxidant. Dysregulation of these transporters has been implicated in various pathological conditions, including cancer and neurodegenerative diseases.

The HEK293 cell line, derived from human embryonic kidney, is widely used for the expression of recombinant proteins and the study of gene function. When stably expressing the mouse Slc7a9/Slc3a1 genes, the HEK293 cell line serves as an excellent model for investigating the molecular mechanisms of cystine and glutamate transport, as well as their implications in disease. This cell line is particularly useful for drug screening and the development of therapeutic strategies targeting these transporters.

Leucine-rich repeat kinase 2 (LRRK2) has been linked to dopaminergic neuronal vulnerability to oxidative stress (OS), mitochondrial impairment, and increased cell death in both idiopathic and familial Parkinson's disease (PD). However, the exact mechanism by which LRRK2 contributes to these processes remains unclear. The researchers utilized CRISPR/Cas9 technology to generate LRRK2 knockout (KO) HEK-293 cells and analyzed their response to the mitochondrial inhibitor rotenone (ROT), a known inducer of OS and cell death. By comparing LRRK2 wild-type (WT) and KO cells, they found that LRRK2 KO cells showed resistance to ROT-induced OS and apoptosis, suggesting that LRRK2 plays a key role in regulating mitochondrial dysfunction and apoptosis in response to oxidative stress. This observation supports the hypothesis that LRRK2 is a pro-apoptotic kinase involved in PD pathogenesis.

Figure 1 describes the cryo-EM structure of the b(0,+)AT1-rBAT transport complex, detailing its heterotetrameric assembly, secondary structure motifs, and subunit interactions. (doi: 10.1073/pnas.2008111117) Figure 1. The researchers used cryo-electron microscopy (cryo-EM) to determine the structure of the b(0,+)AT1-rBAT complex. They revealed a heterotetrameric assembly of two rBAT and b(0,+)AT1 subunits and mapped key structural features, including glycosylation sites, disulfide bonds, and subunit interactions, providing insights into the transport mechanism. (Wu D, et al., 2020)

Creative Biogene's Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 is an ideal tool for studying similar pathways related to amino acid transporters and their role in disease mechanisms. The cell line is engineered to express both the SLC3A1 and SLC7A9 genes, enabling research on the b(0,+)AT1-rBAT complex involved in L-arginine transport.

The Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 product is developed based on the human embryonic kidney cell line HEK293, specifically constructed through the stable transfection of the mouse Slc7a9 and Slc3a1 genes. The proteins encoded by these genes are crucial components of amino acid transporters, essential for maintaining the balance of amino acids inside and outside the cell. Below are detailed introductions to three unique application areas of this product:

（1）Drug Screening and Pharmacokinetics Research: The Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 can be used for drug screening, especially for drugs targeting amino acid transporters. This cell line can be utilized to assess how drugs affect amino acid transport and whether drugs can exert their effects by altering the concentration of amino acids inside and outside the cell. Moreover, this cell line can also be used to study the absorption, distribution, metabolism, and excretion (ADME) properties of drugs, particularly when the mechanism of action of the drug involves amino acid transport.

（2）Research on Genetic Metabolic Diseases: Due to the role of Slc7a9 and Slc3a1 genes in amino acid transport, the Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 becomes an ideal tool for researching certain genetic metabolic diseases, especially those related to the dysregulation of amino acid transport. Using this cell line, researchers can explore how specific mutations affect amino acid transport, thereby revealing the molecular mechanisms of the disease and aiding in the development of targeted therapeutic strategies.

（3）Cell Biology and Metabolism Research: Beyond drug development and disease research, the Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 can also be used for broader cell biology and metabolism studies. For instance, researchers can use this cell line to study the impact of amino acid transport on cell proliferation, differentiation, and death, or to explore how amino acid availability regulates cellular metabolic pathways. Additionally, this cell line can be used to study how cells sense and respond to changes in nutrients, and how these changes affect the physiological and pathological states of the cells.

Customer Q&As

What are the specific functions of the Slc7a9 and Slc3a1 genes in the context of the Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293, and how do they contribute to amino acid transport mechanisms?

A: The Slc7a9 gene encodes for a subunit of the cystine/glutamate exchange transporter, while Slc3a1 encodes for a subunit of the histidine/histamine transport system. In the Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293, these genes work together to facilitate the transport of amino acids across the cell membrane, which is crucial for cellular metabolism and protein synthesis.

How can the Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 be utilized to study the effects of genetic mutations on amino acid transport and the resulting impact on cellular functions?

A: By introducing specific mutations into the Slc7a9 and Slc3a1 genes within the cell line, researchers can investigate how these genetic changes affect the efficiency and regulation of amino acid transport. This can provide insights into the molecular basis of transport-related diseases and potential therapeutic targets.

In what ways can the Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 be applied to develop and test drugs that target amino acid transporters, particularly for conditions like cystinuria?

A: The cell line can be used in drug screening assays to identify compounds that modulate the activity of the amino acid transporters encoded by Slc7a9 and Slc3a1. By assessing the effects of these compounds on transporter function, researchers can optimize potential therapeutic agents for the treatment of cystinuria and other related disorders.

How does the Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 model contribute to our understanding of the broader biological processes involving amino acid transport, such as protein synthesis and cellular signaling?

A: The cell line provides a controlled environment to study the role of amino acid transporters in various biological processes. By observing how changes in transporter function affect protein synthesis and signaling pathways, researchers can gain a deeper understanding of the complex interplay between these processes and their implications for cellular health.

What are the potential applications of the Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 in the field of systems biology, particularly for mapping the interactions between amino acid transport and other cellular components?

A: In systems biology, the cell line can be used to construct networks of molecular interactions involving amino acid transporters. This can help researchers identify key regulatory nodes and potential points of intervention for modulating cellular processes related to amino acid transport.

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Customer Reviews

High Expression Levels

The Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 is engineered to ensure high-efficiency expression of Slc7a9 and Slc3a1 genes in HEK293 cells.

Canada

2020-01-23

Genetic Stability

This product maintains good genetic stability through passaging, ensuring consistency and reproducibility for long-term experiments.

United Kingdom

2021-12-13

Easy Selection

The built-in selection marker in Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 makes the selection of stable expressing cells straightforward.

United Kingdom

2022-12-19

Functional Validation

We can efficiently perform functional analysis of Slc7a9/Slc3a1 with this product to explore its role in cellular physiology.

Germany

2021-06-08

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Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293

Cell Line Information

Cell Culture Information

Safety and Packaging

Documents

Background

Case Study

Applications

Q & A

Customer Reviews

What are the specific functions of the Slc7a9 and Slc3a1 genes in the context of the Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293, and how do they contribute to amino acid transport mechanisms?

How can the Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 be utilized to study the effects of genetic mutations on amino acid transport and the resulting impact on cellular functions?

In what ways can the Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 be applied to develop and test drugs that target amino acid transporters, particularly for conditions like cystinuria?

How does the Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 model contribute to our understanding of the broader biological processes involving amino acid transport, such as protein synthesis and cellular signaling?

What are the potential applications of the Mouse Slc7a9/Slc3a1 Stable Cell Line - HEK293 in the field of systems biology, particularly for mapping the interactions between amino acid transport and other cellular components?

High Expression Levels

Genetic Stability

Easy Selection

Functional Validation

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