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Premade AAV, adenovirus, lentivirus particles, safe, stable, in stock.
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Human Druggable Genome siRNA Library enables efficient drug target screening.
Recombinant Drug Target Proteins
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Providing functional, high-purity recombinant proteins—including membrane proteins and nanodiscs—to overcome bottlenecks in drug screening and target validation.
Clones
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Ready-to-use clones for streamlined research and development.
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Chromogenic LAL Endotoxin Assay Kit ensures precise, FDA-compliant endotoxin quantification for biosafety testing.
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Powerful Tn5 Transposase for DNA insertion and random library construction.
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Aptamers for key proteins like ACVR1A, Akt, EGFR, and VEGFR.
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Enhance immune responses with high-purity, potent CpG ODNs.
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Unbeatable pricing, fully customizable viral packaging services (covering 30,000+ human genes, 200+ mammals, 50+ protein tags).
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Protein Degrader Service
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Harness the power of protein degraders for precise protein degradation, expanding druggable targets and enhancing therapeutic effectiveness for cutting-edge drug discovery.
Nucleotides Service
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Custom synthesis of oligonucleotides, primers, and probes for gene editing, PCR, and RNA studies.
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Custom RNA ServicePrecise | Flexible | GMP-ReadyCustom
RNA design, synthesis, and manufacturing—covering mRNA, saRNA, circRNA, and RNAi. Fast turnaround, rigorous QC, and seamless transition from research to GMP production.
Custom Libraries Construction Service
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Custom cDNA, genomic, and mutagenesis libraries for drug discovery, screening, and functional genomics.
Gene Editing Services
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Gene editing solutions for gene editing, knockouts, knock-ins, and customized genetic modifications. Integrated multi-platform solutions for one-stop CRISPR sgRNA library synthesis and gene screening services
Microbe Genome Editing Service
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Enhance microbial productivity with advanced genome editing using Rec-mediated recombination and CRISPR/Cas9 technologies.
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Plant-based protein expression systems for biopharmaceuticals, enzyme production, and research.
Aptamers Service
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Revolutionizing drug delivery and diagnostic development with next-generation high-throughput aptamer selection and synthesis technologies.
CGT Biosafety Testing
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Internationally certified evaluation system for biologics, gene therapies, nucleic acid drugs, and vaccines.
Pandemic Detection Solutions
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Stable expression over 15 generations with rapid cell line development in just 3 months.
Supports adherent and suspension cell lines, offering MCB, WCB, and PCB establishment.
GMP mRNA Production
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Scalable mRNA production from milligrams to grams, with personalized process design for sequence optimization, cap selection, and nucleotide modifications, all in one service.
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Our plasmid production services span Non-GMP, GMP-Like, and GMP-Grade levels, with specialized options for linearized plasmids.
GMP Viral Vector Manufacturing
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Advanced platforms for AAV, adenovirus, lentivirus, and retrovirus production, with strict adherence to GMP guidelines and robust quality control.
AI-Driven Gene Editing and Therapy
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AI-powered one-click design for customized CRISPR gene editing strategy development.
AI-Antibody Engineering Fusion
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AI and ML algorithms accelerate antibody screening and predict new structures, unlocking unprecedented possibilities in antibody engineering.
AI-Driven Enzyme Engineering
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High-throughput enzyme activity testing with proprietary datasets and deep learning models for standardized and precise enzyme engineering design.
AI-Enhanced Small Molecule Screening
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Leverage AI to uncover hidden high-potential small molecules, prioritize leads intelligently, and reduce costly trial-and-error in early drug discovery.
AI-Driven Protein Degrader Drug Development
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Use AI-guided design to optimize protein degraders, addressing design complexity and enhancing efficacy while shortening development timelines.
Cat.No. : CSC-RO1062 Host Cell: HEK293T
| Cat. No. | CSC-RO1062 |
| Description | HEK293-Cd274 cell line is engineered to stably overexpress mouse Cd274. |
| Gene | Cd274 |
| Gene Species | Mus musculus (Mouse) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Use high-specificity PD-L1 antibodies for flow cytometry or Western Blot analysis, and set appropriate internal reference proteins or control cell lines to calibrate the results.
A: Use physical isolation methods, such as transwell culture systems, or chemical inhibitors to reduce non-specific cell interactions, ensuring the specificity of the experiment.
A: Optimize drug treatment conditions, such as drug concentration and duration, and adjust cell density and culture conditions to increase cell sensitivity to drugs.
A: Optimize cell lysis conditions and immunoprecipitation reagent choices, use high-specificity antibodies, and ensure sufficient incubation time and gentle washing steps.
A: Optimize the choice of antibodies and incubation conditions, such as antibody concentration and incubation time, and ensure cell viability during processing and analysis.
A: Precisely design sgRNA, improve the expression efficiency of Cas9, use efficient transfection methods, and conduct rigorous off-target analysis to ensure the specificity of gene editing.
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