Cat.No. : CSC-SC016312-1 Host Cell: MDCK
| Cat. No. | CSC-SC016312-1 |
| Description | This cell line is engineered to stably express human transmembrane serine protease 11E (TMPRSS11E) in MDCK cells. |
| Gene | TMPRSS11E |
| Gene Species | Homo sapiens (Human) |
| Host Cell | MDCK |
| Host Cell Species | Canis lupus familiaris (Dog) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The TMPRSS11E gene encodes for the transmembrane serine protease 11E, a type II transmembrane serine protease. This enzyme is primarily expressed in the respiratory epithelium and is thought to play a role in the proteolytic processing of proteins within the airway lining fluid.
A: The TMPRSS11E gene product may contribute to respiratory health by maintaining the balance of proteins in the airway lining fluid. It is believed to be involved in the regulation of mucus viscosity and the degradation of harmful substances, such as bacteria and viruses, that are inhaled into the lungs.
A: Currently, there are no direct associations between mutations in the TMPRSS11E gene and specific diseases. However, as a component of the respiratory protease/antiprotease system, dysfunction of TMPRSS11E could potentially disrupt the protective mechanisms of the lungs and contribute to respiratory disorders.
A: The exact mechanisms of regulation for the TMPRSS11E gene in the respiratory epithelium are not fully understood. It is likely that the expression of TMPRSS11E is influenced by the local microenvironment, including the presence of inflammatory signals, pathogens, and the composition of the airway lining fluid.
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The Human TMPRSS11E Stable Cell Line - MDCK exhibits long-term stability of editing events, with the knockout phenotype remaining consistent over numerous passages. This persistence is critical for long-term studies and ensures the reliability of the cell line for ongoing research projects.
The editing events in this cell line are successfully transmitted to subsequent generations, allowing for the study of TMPRSS11E gene inheritance and its impact on related biological processes. This transmissibility is invaluable for understanding the gene's role in both Mendelian and complex diseases.
The technology used to generate the Human TMPRSS11E Stable Cell Line - MDCK is scalable, enabling the production of large quantities of edited cells for high-throughput screening and extensive research applications. This scalability makes the cell line a powerful tool for both basic and translational research.
The Human TMPRSS11E Stable Cell Line - MDCK is produced using cost-effective gene editing technology, making it an affordable option for researchers working with limited budgets. Its cost-effectiveness promotes wider accessibility and allows more laboratories to benefit from this valuable research tool.
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