Human TAS2R39 Stable Cell Line - HEK293

Name: Human TAS2R39 Stable Cell Line - HEK293
SKU: CSC-SC015603-1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC015603-1

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC015603-1
Description	This cell line is engineered to stably express human taste 2 receptor member 39 (TAS2R39) in HEK293 cells.
Target Gene	TAS2R39
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TAS2R39 taste receptor, type 2, member 39 [ Homo sapiens ]
Gene Symbol	TAS2R39
Synonyms	T2R39; T2R57
Gene ID	259285
Uni Prot ID	P59534
m RNA Refseq	NM_176881.2
Protein Refseq	NP_795362.2
Chromosome Location	7q34
Function	G-protein coupled receptor activity;
Pathway	Taste transduction, organism-specific biosystem; Taste transduction, conserved biosystem;

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Vanilla is widely used in food preparations around the world for its sensory properties, which are mainly related to its aroma, with vanillin being the main compound in processed vanilla. It is well known that vanillin also causes bitterness, a secondary sensory sensation, but the molecular mechanism underlying its bitter taste has never been reported. An assay buffer for vanillin was tested against all 25 known human bitter taste receptors, TAS2R, in vitro. Three receptors, TAS2R14, TAS2R20 and TAS2R39, were activated, indicating that these receptors are mediating the bitter taste of vanillin. The results may help improve the overall sensory profile of this widely used food ingredient, but more importantly can serve as a starting point for further studies to explore the potential of vanillin in sensory nutrition and other pharmaceutical applications.

Dose-response curves were obtained after stimulation of HEK293 PEAKrapid Gα16Gi/o44 cells overexpressing TAS2R14, TAS2R20, and TAS2R39 bitter receptors.

Figure 1. Activity of vanillin on human TAS2R14, TAS2R20, and TAS2R39 bitter taste receptors. (A) The graph shows dose-response curves obtained after stimulation of HEK293 PEAKrapid Gα16Gi/o44 cells overexpressing TAS2R14 (black filled triangles), TAS2R20 (black filled squares), and TAS2R39 (black filled diamonds). (B) Calcium responses of TAS2R14 (solid line), TAS2R20 (dashed line), and TAS2R39 (dash-dotted line) expressing cells to 3 mM vanillin. Calcium indicators were loaded in receptor-expressing cells, and fluorescence emission was recorded before and after exposure of cells to vanillin. (Morini G, et al. 2021)

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