Human TAS2R39 Stable Cell Line - HEK293

The TAS2R39 gene, officially designated as taste 2 receptor member 39, is located on chromosome 7q34 in humans and encodes a member of the TAS2R family of bitter taste receptors. This intronless gene produces a protein of approximately 326 amino acids that functions as a G protein-coupled receptor (GPCR) primarily expressed in taste receptor cells of the tongue. The TAS2R39 receptor is known to recognize and respond to a range of naturally occurring bitter compounds, including green tea catechins, soy isoflavones, and theaflavins. It signals through gustducin, a taste-specific G protein, and is thought to play a significant role in the detection of dietary bitter substances. The Human TAS2R39 Stable Cell Line - HEK293 is generated by stably transfecting the full-length human TAS2R39 gene into HEK293 cells, a widely used human embryonic kidney cell line valued for its reliable growth, high transfection efficiency, and robust protein expression. These cells constitutively express functional TAS2R39 on the plasma membrane, providing a consistent and reproducible in vitro system for studying receptor activation, ligand binding, and downstream signaling pathways.

This stable cell line serves as a valuable tool for a range of research applications. It is widely employed in bitter taste receptor pharmacology, including the screening and characterization of agonists and antagonists. Researchers use this cell-based assay to evaluate the bitterness potential of food ingredients, natural products, pharmaceuticals, and flavor-masking agents. The line is also useful in structure-activity relationship studies, helping to define the molecular features required for ligand recognition by TAS2R39. Because the receptor is implicated in the detection of bioactive dietary compounds, the cell line supports investigations into the role of bitter taste signaling in nutrition, gut chemosensation, and metabolic regulation. Additionally, it can be used in GPCR signaling studies to examine coupling efficiency, desensitization, and cross-talk with other pathways. The stable nature of the expression ensures reproducibility across experiments, making it a practical choice for both academic research and industrial drug discovery programs focused on taste modulation and sensory science.