Human SLC6A2 Stable Cell Line - HEK293

Name: Human SLC6A2 Stable Cell Line - HEK293
SKU: CSC-SC014633-1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC014633-1

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC014633-1
Description	This cell line is engineered to stably express human solute carrier family 6 member 2 (SLC6A2) in HEK293 cells.
Target Gene	SLC6A2
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SLC6A2 solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 [ Homo sapiens ]
Gene Symbol	SLC6A2
Synonyms	NET; NAT1; NET1; SLC6A5
Gene ID	6530
Uni Prot ID	B4DX48
m RNA Refseq	NM_001043.3
Protein Refseq	NP_001034.1
Chromosome Location	16q12.2
Function	monoamine transmembrane transporter activity; norepinephrine:sodium symporter activity; protein binding;
Pathway	Monoamine Transport, organism-specific biosystem; Na+/Cl- dependent neurotransmitter transporters, organism-specific biosystem; SLC-mediated transmembrane transport, organism-specific biosystem; Transmembrane transport of small molecules, organism-specific biosystem; Transport of glucose and other sugars, bile salts and organic acids, metal ions and amine compounds, organism-specific biosystem;
MIM	163970

Quick Inquiry

Background

Applications

Q & A

Customer Reviews

The Human SLC6A2 gene encodes the norepinephrine transporter (NET), which is responsible for the reuptake of norepinephrine from the synaptic cleft back into the presynaptic neuron. NET plays a pivotal role in the regulation of the noradrenergic system, affecting mood, attention, and stress response. Alterations in NET function are associated with various psychiatric disorders, including depression and attention deficit hyperactivity disorder (ADHD).

The HEK293 cell line, when stably expressing the human SLC6A2 gene, provides a valuable tool for studying the regulation of norepinephrine transport and the effects of drugs that modulate NET activity. This cell line is instrumental for the development of new treatments for disorders related to the noradrenergic system and for understanding the molecular basis of NET function and dysfunction.

The Human SLC6A2 Stable Cell Line - HEK293 is a specialized cell line engineered to stably express the SLC6A2 gene, which encodes the serotonin transporter. This cell line is instrumental in various research and therapeutic applications due to its unique genetic makeup and functionality.

（1） Neuropharmacological Research:

The SLC6A2 Stable Cell Line is extensively used in neuropharmacological research to study the mechanisms of action of serotonin and its role in various neurological disorders. Researchers utilize these cells to screen for potential drugs targeting the serotonin transporter, which is implicated in depression, anxiety, and other mood disorders. By observing the interaction between test compounds and the serotonin transporter, scientists can evaluate the efficacy and safety of new therapeutic agents.

（2）Toxicology and Drug Development:

In the field of toxicology, this cell line serves as a model for assessing the cardiotoxic potential of drugs, as the serotonin transporter plays a role in cardiac function. By monitoring the effects of various compounds on the activity of the serotonin transporter, researchers can predict potential side effects on the cardiovascular system. This cell line is also valuable in the early stages of drug development for filtering out compounds with undesirable cardiotoxic properties.

（3） Cell Signaling and Molecular Biology Studies:

The HEK293 cell line expressing SLC6A2 is used to investigate the intracellular signaling pathways modulated by serotonin transport. These studies are crucial for understanding the complex interactions between neurotransmitters and their receptors, which can lead to the identification of novel targets for therapeutic intervention. Additionally, the manipulation of the SLC6A2 gene in these cells can provide insights into the molecular mechanisms underlying neurotransmission and the development of neurodegenerative diseases.

Customer Q&As

How does the Human SLC6A2 Stable Cell Line - HEK293 model the role of the serotonin transporter in neuronal function and its implications in neuropsychiatric disorders?

A: The cell line stably expresses the SLC6A2 gene, which encodes the serotonin transporter. This allows researchers to study the transporter's role in serotonin reuptake, a critical process in regulating neuronal signaling. By manipulating the SLC6A2 gene, researchers can investigate the impact of serotonin transport on neuropsychiatric disorders like depression and anxiety.

In the context of drug development, how can the Human SLC6A2 Stable Cell Line - HEK293 be used to identify compounds that modulate serotonin transport, potentially leading to new treatments for mood disorders?

A: The cell line can be utilized in high-throughput screening assays to test the effects of various compounds on serotonin transport. By monitoring changes in serotonin levels, researchers can identify potential drug candidates that either enhance or inhibit serotonin reuptake, which could be beneficial for developing new therapeutics for mood disorders.

What are the implications of using the Human SLC6A2 Stable Cell Line - HEK293 for studying the pharmacokinetics and pharmacodynamics of serotonin reuptake inhibitors?

A: The cell line provides a platform for evaluating the absorption, distribution, metabolism, and excretion of potential serotonin reuptake inhibitors. Additionally, it can be used to study the dynamic effects of these inhibitors on serotonin transport, offering insights into their therapeutic potential and potential side effects.

How can the Human SLC6A2 Stable Cell Line - HEK293 contribute to the understanding of the molecular mechanisms underlying serotonin transporter regulation and its response to environmental stimuli?

A: By exposing the cell line to various environmental stimuli and assessing changes in SLC6A2 gene expression and serotonin transport activity, researchers can uncover the molecular mechanisms that govern transporter regulation. This can lead to a better understanding of how external factors influence serotonin signaling and neuronal function.

What role can the Human SLC6A2 Stable Cell Line - HEK293 play in investigating the interactions between the serotonin transporter and other neurotransmitter systems in the brain?

A: The cell line can be used to study the crosstalk between serotonin transport and other neurotransmitter systems, such as dopamine and norepinephrine. By examining how these systems interact and influence each other, researchers can gain insights into the complex neural circuits involved in mood regulation and cognitive function.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Customer Reviews

Enhanced Noradrenaline Study

The Human SLC6A2 Stable Cell Line - HEK293 provides a targeted model for studying noradrenaline reuptake, crucial for understanding mood disorders and the action of antidepressants.

Canada

2024-04-26

High Expression Levels

This cell line ensures robust expression of SLC6A2, facilitating efficient assay development for screening SLC6A2 interactors or inhibitors.

French

2022-09-25

Relevant Transporter Assays

Using Human SLC6A2 Stable Cell Line - HEK293 allows for relevant transporter assays, essential for psychopharmacological research.

Germany

2023-09-19

Pharmacokinetic Modeling

It enables precise pharmacokinetic modeling of drugs affecting the noradrenergic system, pivotal for CNS drug development.

United Kingdom

2023-12-27

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *

Name

Country *

Email *

Related Products

Cat.No.	Product Name	Price
CSC-SC000015	Panoply™ Human AAMDC Over-expressing Stable Cell Line	Inquiry
CSC-SC000055	Panoply™ Human ABCC4 Over-expressing Stable Cell Line	Inquiry
CSC-SC000064	Panoply™ Human ABCE1 Over-expressing Stable Cell Line	Inquiry
CSC-SC000074	Panoply™ Human ABHD10 Over-expressing Stable Cell Line	Inquiry
CSC-SC000004	Panoply™ Human A4GALT Over-expressing Stable Cell Line	Inquiry
CSC-SC000018	Panoply™ Human AAR2 Over-expressing Stable Cell Line	Inquiry
CSC-SC000023	Panoply™ Human AASDHPPT Over-expressing Stable Cell Line	Inquiry
CSC-SC000108	Panoply™ Human ACAA2 Over-expressing Stable Cell Line	Inquiry
CSC-RO0023	hSLC5A5 Overexpression Stable Cell Line-Mel624	Inquiry
CSC-SC000093	Panoply™ Human ABI3BP Over-expressing Stable Cell Line	Inquiry

Human SLC6A2 Stable Cell Line - HEK293

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

How does the Human SLC6A2 Stable Cell Line - HEK293 model the role of the serotonin transporter in neuronal function and its implications in neuropsychiatric disorders?

In the context of drug development, how can the Human SLC6A2 Stable Cell Line - HEK293 be used to identify compounds that modulate serotonin transport, potentially leading to new treatments for mood disorders?

What are the implications of using the Human SLC6A2 Stable Cell Line - HEK293 for studying the pharmacokinetics and pharmacodynamics of serotonin reuptake inhibitors?

How can the Human SLC6A2 Stable Cell Line - HEK293 contribute to the understanding of the molecular mechanisms underlying serotonin transporter regulation and its response to environmental stimuli?

What role can the Human SLC6A2 Stable Cell Line - HEK293 play in investigating the interactions between the serotonin transporter and other neurotransmitter systems in the brain?

Enhanced Noradrenaline Study

High Expression Levels

Relevant Transporter Assays

Pharmacokinetic Modeling

Related Products

Related Services