Cat.No. : CSC-SC014633-1 Host Cell: HEK293
| Cat. No. | CSC-SC014633-1 |
| Description | This cell line is engineered to stably express human solute carrier family 6 member 2 (SLC6A2) in HEK293 cells. |
| Gene | SLC6A2 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The cell line stably expresses the SLC6A2 gene, which encodes the serotonin transporter. This allows researchers to study the transporter's role in serotonin reuptake, a critical process in regulating neuronal signaling. By manipulating the SLC6A2 gene, researchers can investigate the impact of serotonin transport on neuropsychiatric disorders like depression and anxiety.
A: The cell line can be utilized in high-throughput screening assays to test the effects of various compounds on serotonin transport. By monitoring changes in serotonin levels, researchers can identify potential drug candidates that either enhance or inhibit serotonin reuptake, which could be beneficial for developing new therapeutics for mood disorders.
A: The cell line provides a platform for evaluating the absorption, distribution, metabolism, and excretion of potential serotonin reuptake inhibitors. Additionally, it can be used to study the dynamic effects of these inhibitors on serotonin transport, offering insights into their therapeutic potential and potential side effects.
A: By exposing the cell line to various environmental stimuli and assessing changes in SLC6A2 gene expression and serotonin transport activity, researchers can uncover the molecular mechanisms that govern transporter regulation. This can lead to a better understanding of how external factors influence serotonin signaling and neuronal function.
A: The cell line can be used to study the crosstalk between serotonin transport and other neurotransmitter systems, such as dopamine and norepinephrine. By examining how these systems interact and influence each other, researchers can gain insights into the complex neural circuits involved in mood regulation and cognitive function.
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The Human SLC6A2 Stable Cell Line - HEK293 provides a targeted model for studying noradrenaline reuptake, crucial for understanding mood disorders and the action of antidepressants.
This cell line ensures robust expression of SLC6A2, facilitating efficient assay development for screening SLC6A2 interactors or inhibitors.
Using Human SLC6A2 Stable Cell Line - HEK293 allows for relevant transporter assays, essential for psychopharmacological research.
It enables precise pharmacokinetic modeling of drugs affecting the noradrenergic system, pivotal for CNS drug development.
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