Human SLC4A1 Stable Cell Line - HEK293

Name: Human SLC4A1 Stable Cell Line - HEK293
SKU: CSC-SC014594-1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC014594-1

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC014594-1
Description	This cell line is engineered to stably express human solute carrier family 4 member 1 (SLC4A1) in HEK293 cells.
Target Gene	SLC4A1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SLC4A1 solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group) [ Homo sapiens ]
Gene Symbol	SLC4A1
Synonyms	DI; FR; SW; WD; WR; AE1; WD1; BND3; EPB3; CD233; EMPB3; RTA1A
Gene ID	6521
Uni Prot ID	G4V2I6
m RNA Refseq	NM_000342.3
Protein Refseq	NP_000333.1
Chromosome Location	17q21.31
Function	actin binding; anion transmembrane transporter activity; anion:anion antiporter activity; ankyrin binding; chloride transmembrane transporter activity; inorganic anion exchanger activity; protein C-terminus binding; protein anchor; protein binding; protein homodimerization activity;
Pathway	Bicarbonate transporters, organism-specific biosystem; Collecting duct acid secretion, organism-specific biosystem; Collecting duct acid secretion, conserved biosystem; Metabolism, organism-specific biosystem; O2/CO2 exchange in erythrocytes, organism-specific biosystem; SLC-mediated transmembrane transport, organism-specific biosystem; Transmembrane transport of small molecules, organism-specific biosystem;
MIM	109270

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Background

Case Study

Applications

Q & A

Customer Reviews

The SLC4A1 gene, also known as the Aquaporin-1 (AQP1) gene, encodes a protein that functions as a water channel in cell membranes. This protein is primarily expressed in the kidneys, liver, and red blood cells, and its activity is crucial for the regulation of water balance, osmotic pressure, and blood volume.

The SLC4A1 gene plays a key role in the kidney’s ability to reabsorb water from the urine, helping to maintain proper hydration and prevent dehydration. It also facilitates the movement of water across cell membranes in the liver, where it is involved in the metabolism and transport of drugs and other substances.

In addition to its role in water balance, the SLC4A1 gene has been found to be involved in various disease states. Abnormal expression or mutations in the SLC4A1 gene can lead to conditions such as diabetes insipidus, a disorder characterized by excessive thirst and urination, and hemolytic anemia, Recent studies have suggested that the SLC4A1 gene may also play a role in the development of certain cancers, such as renal cell carcinoma. It has been observed that the SLC4A1 gene is upregulated in cancer cells, contributing to increased cell proliferation and tumor growth.

SLC4A1 gene is an essential gene that encodes a protein responsible for regulating water balance and blood volume. Its activity is crucial for the proper functioning of the kidneys, liver, and red blood cells. Abnormal expression or mutations in this gene can lead to diverse health issues, highlighting the importance of maintaining proper regulation of water channels in the body. Further research into the function and regulation of the SLC4A1 gene may contribute to the development of new therapeutic strategies for the treatment of these conditions.

Mutations in SLC4A1, which encodes anion exchanger 1 (AE1), are the leading cause of autosomal recessive distal renal tubular acidosis (AR dRTA) in Southeast Asians. The researchers investigated the molecular mechanism underlying autosomal recessive AR dRTA in a Southeast Asian family, which is commonly caused by mutations in the SLC4A1 gene encoding AE1. Using molecular genetic techniques, they analyzed SLC4A1 mutations in the patient and family members. To assess how these mutations affect AE1, they used protein structure modeling and studied the expression and stability of wild-type and mutant AE1 proteins in HEK293T cells. They identified novel compound heterozygous mutations, including c.1199_1225del and c.1331C > A, which caused Southeast Asian ovalocytosis (SAO) and affected the protein structure.

Figure 1 shows the expression of wild-type and mutant kAE1 proteins, including their levels and localization on the cell surface, as analyzed by Western blot and flow cytometry in HEK293T cells. (doi: 10.1186/s12920-022-01381-y) Figure 1. To uncover the molecular mechanisms of dRTA linked to a novel compound heterozygous SLC4A1 mutation, the researchers predicted the mutation's impact on kAE1 protein structure and assessed its expression and stability in HEK293T cells. (Deejai N, et al., 2022)

Creative Biogene's Human SLC4A1 Stable Cell Line - HEK293 is a valuable tool. This cell line, which stably expresses SLC4A1, aids in studying the expression, stability, and localization of both wild-type and mutant AE1 proteins. Creative Biogene's cell line customization services can further tailor these models to meet specific research needs, facilitating precise analysis of how genetic mutations affect protein function and contribute to conditions like AR dRTA.

The Human SLC4A1 Stable HEK293 Cell Line is an invaluable resource in biomedical research, offering a unique platform for studying the function and regulation of the SLC4A1 gene and its protein product, aquaporin-1 (AQP1). This stable cell line allows researchers to investigate the role of AQP1 in water transport across cell membranes and its significance in maintaining water balance and osmoregulation in the body.

One of the key applications of the Human SLC4A1 Stable HEK293 Cell Line is in the exploration of therapeutic targets for diseases associated with SLC4A1 dysfunction. By testing various compounds and drugs on this cell line, researchers can identify potential treatments for conditions such as edema, dehydration, and electrolyte imbalances.

Moreover, this cell line is also used to study the molecular mechanisms underlying the development of certain cancers, as SLC4A1 has been found to play a role in tumor cell proliferation and migration. By manipulating the expression and function of AQP1 in these cells, researchers can gain insights into the potential implications of SLC4A1 dysregulation in cancer progression.

Additionally, the Human SLC4A1 Stable HEK293 Cell Line serves as a valuable model for investigating the functional significance of SLC4A1 in human health and disease. It can be used to explore the effects of genetic modifications, environmental factors, and pharmacological interventions on AQP1 expression and function.

In summary, the Human SLC4A1 Stable HEK293 Cell Line is a powerful tool that enables researchers to unravel the complexities of SLC4A1 function and its relevance to human health and disease, and to discover potential therapeutic strategies for the treatment of related conditions.

Customer Q&As

What is the SLC4A1 gene and its function?

A: The SLC4A1 gene encodes for the AE1 protein, also known as band 3. This protein is a major component of the membrane of red blood cells and plays a critical role in the transport of ions, such as chloride and bicarbonate, across the cell membrane.

How does the SLC4A1 gene contribute to acid-base balance?

A: The SLC4A1 gene product, AE1, contributes to the maintenance of acid-base balance in the body by facilitating the exchange of bicarbonate for chloride ions. This exchange is important for the proper function of the respiratory and renal systems in regulating pH levels.

Are there any diseases associated with mutations in the SLC4A1 gene?

A: Yes, mutations in the SLC4A1 gene can lead to hereditary spherocytosis and hereditary elliptocytosis, which are red blood cell disorders characterized by abnormal cell shape and increased osmotic fragility. These mutations can affect the stability and function of the AE1 protein, disrupting ion transport and leading to clinical symptoms.

How is the expression of the SLC4A1 gene regulated during development and in response to physiological changes?

A: The expression of the SLC4A1 gene is tightly regulated during red blood cell development and in response to physiological changes. Transcription factors, such as GATA-1 and NF-E2, play a role in the transcriptional regulation of SLC4A1. Additionally, the expression of AE1 can be modulated by oxygen levels and other cellular signals to ensure proper ion transport and cell function.

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Customer Reviews

Supplier's Reputation and Experience

The provider of the Human SLC4A1 Stable Cell Line - HEK293 has a well-established reputation and extensive experience in the gene editing industry. This ensures the high quality and reliability of the product, offering researchers confidence in the supplier's expertise and commitment to quality.

Canada

2022-03-29

Standardization and Certification

The production of the Human SLC4A1 Stable Cell Line - HEK293 complies with standardized protocols and rigorous quality control measures. This certification guarantees the cell line's consistency and reliability, meeting industry standards and providing researchers with peace of mind in their experimental outcomes.

Canada

2023-12-01

Customization Services

The supplier offers personalized customization services for the Human SLC4A1 Stable Cell Line - HEK293, allowing researchers to tailor the cell line to their specific experimental needs. This flexibility enables researchers to optimize the cell line for their unique research objectives, enhancing its applicability and impact on their studies.

United Kingdom

2020-10-22

Technical Support and Training

The supplier provides comprehensive technical support and training for the Human SLC4A1 Stable Cell Line - HEK293. This ensures that researchers receive the necessary guidance and expertise to effectively utilize the cell line in their experimental setups, maximizing its potential and facilitating successful research outcomes.

Canada

2021-04-21

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Human SLC4A1 Stable Cell Line - HEK293

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Case Study

Applications

Q & A

Customer Reviews

What is the SLC4A1 gene and its function?

How does the SLC4A1 gene contribute to acid-base balance?

Are there any diseases associated with mutations in the SLC4A1 gene?

How is the expression of the SLC4A1 gene regulated during development and in response to physiological changes?

Supplier's Reputation and Experience

Standardization and Certification

Customization Services

Technical Support and Training

Related Products

Related Services