Cat.No. : CSC-SC014594-1 Host Cell: HEK293
| Cat. No. | CSC-SC014594-1 |
| Description | This cell line is engineered to stably express human solute carrier family 4 member 1 (SLC4A1) in HEK293 cells. |
| Gene | SLC4A1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Mutations in SLC4A1, which encodes anion exchanger 1 (AE1), are the leading cause of autosomal recessive distal renal tubular acidosis (AR dRTA) in Southeast Asians. The researchers investigated the molecular mechanism underlying autosomal recessive AR dRTA in a Southeast Asian family, which is commonly caused by mutations in the SLC4A1 gene encoding AE1. Using molecular genetic techniques, they analyzed SLC4A1 mutations in the patient and family members. To assess how these mutations affect AE1, they used protein structure modeling and studied the expression and stability of wild-type and mutant AE1 proteins in HEK293T cells. They identified novel compound heterozygous mutations, including c.1199_1225del and c.1331C > A, which caused Southeast Asian ovalocytosis (SAO) and affected the protein structure.
Figure 1. To uncover the molecular mechanisms of dRTA linked to a novel compound heterozygous SLC4A1 mutation, the researchers predicted the mutation's impact on kAE1 protein structure and assessed its expression and stability in HEK293T cells. (Deejai N, et al., 2022)
Creative Biogene's Human SLC4A1 Stable Cell Line - HEK293 is a valuable tool. This cell line, which stably expresses SLC4A1, aids in studying the expression, stability, and localization of both wild-type and mutant AE1 proteins. Creative Biogene's cell line customization services can further tailor these models to meet specific research needs, facilitating precise analysis of how genetic mutations affect protein function and contribute to conditions like AR dRTA.
A: The SLC4A1 gene encodes for the AE1 protein, also known as band 3. This protein is a major component of the membrane of red blood cells and plays a critical role in the transport of ions, such as chloride and bicarbonate, across the cell membrane.
A: The SLC4A1 gene product, AE1, contributes to the maintenance of acid-base balance in the body by facilitating the exchange of bicarbonate for chloride ions. This exchange is important for the proper function of the respiratory and renal systems in regulating pH levels.
A: Yes, mutations in the SLC4A1 gene can lead to hereditary spherocytosis and hereditary elliptocytosis, which are red blood cell disorders characterized by abnormal cell shape and increased osmotic fragility. These mutations can affect the stability and function of the AE1 protein, disrupting ion transport and leading to clinical symptoms.
A: The expression of the SLC4A1 gene is tightly regulated during red blood cell development and in response to physiological changes. Transcription factors, such as GATA-1 and NF-E2, play a role in the transcriptional regulation of SLC4A1. Additionally, the expression of AE1 can be modulated by oxygen levels and other cellular signals to ensure proper ion transport and cell function.
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The provider of the Human SLC4A1 Stable Cell Line - HEK293 has a well-established reputation and extensive experience in the gene editing industry. This ensures the high quality and reliability of the product, offering researchers confidence in the supplier's expertise and commitment to quality.
The production of the Human SLC4A1 Stable Cell Line - HEK293 complies with standardized protocols and rigorous quality control measures. This certification guarantees the cell line's consistency and reliability, meeting industry standards and providing researchers with peace of mind in their experimental outcomes.
The supplier offers personalized customization services for the Human SLC4A1 Stable Cell Line - HEK293, allowing researchers to tailor the cell line to their specific experimental needs. This flexibility enables researchers to optimize the cell line for their unique research objectives, enhancing its applicability and impact on their studies.
The supplier provides comprehensive technical support and training for the Human SLC4A1 Stable Cell Line - HEK293. This ensures that researchers receive the necessary guidance and expertise to effectively utilize the cell line in their experimental setups, maximizing its potential and facilitating successful research outcomes.
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