Human SLC2A2 Stable Cell Line - CHO-K1

Name: Human SLC2A2 Stable Cell Line - CHO-K1
SKU: CSC-SC014484-1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC014484-1

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC014484-1
Description	This cell line is engineered to stably express human solute carrier family 2 member 2 (SLC2A2) in CHO-K1 cells.
Target Gene	SLC2A2
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SLC2A2 solute carrier family 2 (facilitated glucose transporter), member 2 [ Homo sapiens ]
Gene Symbol	SLC2A2
Synonyms	GLUT2
Gene Description	solute carrier family 2 (facilitated glucose transporter), member 2
GeneID	6514
Uni ProtID	P11168
mRNA Refseq	NM_000340.1
Protein Refseq	NP_000331.1
Chromosome Location	3q26.1-q26.2
Function	D-glucose transmembrane transporter activity; dehydroascorbic acid transporter activity; glucose transmembrane transporter activity; hexose transmembrane transporter activity; insulin receptor binding;
Pathway	Carbohydrate digestion and absorption, organism-specific biosystem; Carbohydrate digestion and absorption, conserved biosystem; Developmental Biology, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Facilitative Na+-independent glucose transporters, organism-specific biosystem; Glucose transport, organism-specific biosystem; Glycolysis and Gluconeogenesis, organism-specific biosystem;
MIM	138160

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Background

Case Study

Applications

Q & A

Customer Reviews

SLC2A2, also known as GLUT2 or glucose transporter type 2, is a transmembrane carrier protein encoded by the SLC2A2 gene. It functions as a facilitative glucose transporter with high capacity but relatively low affinity, making it a key component of the glucose sensing machinery in the body. GLUT2 is primarily expressed in the liver, pancreatic beta cells, basolateral membrane of intestinal epithelial cells, and renal tubular cells. It serves as the principal transporter responsible for moving glucose between the liver and the bloodstream, and also facilitates the transport of fructose and glucosamine. The Human SLC2A2 Stable Cell Line - CHO-K1 is a stably transfected Chinese hamster ovary cell line engineered to constitutively express full-length human SLC2A2. This cell line provides a reliable and reproducible in vitro system for studying GLUT2 function, regulation, and pharmacology without the interference of endogenous glucose transporters commonly found in human-derived cell lines.

This stable cell line serves as a versatile tool for a wide range of research applications. It is particularly valuable for glucose uptake assays, transporter kinetics studies, and investigating the regulatory mechanisms of GLUT2 trafficking and membrane localization. Researchers can use this system to screen and characterize small molecule inhibitors or modulators of GLUT2 activity, which may have therapeutic potential for metabolic disorders such as type 2 diabetes, obesity, and Fanconi-Bickel syndrome. The cell line also supports studies on the role of GLUT2 in hepatic glucose output, insulin secretion coupling, and intestinal sugar absorption. Additionally, it can be employed in mechanistic studies exploring how GLUT2 dysfunction contributes to diabetic complications and metabolic dysregulation. With its stable and consistent expression of human SLC2A2, this cell line reduces experimental variability and provides a dependable platform for both basic research and drug discovery efforts aimed at targeting glucose transport pathways.

Perinatal mice lacking Rfx3 show significantly fewer insulin-producing β-cells in pancreatic islets. Researchers aimed to uncover the mechanisms underlying reduced insulin-producing β-cells in Rfx3-deficient mice. They found that deficiency didn't affect endocrine progenitors or β-cell specification, but led to accumulation of immature insulin-positive cells. Defective β-cells lacked Glut-2 and Gck, causing glucose intolerance. Inhibition of Rfx3 in Min6 cells mirrored these defects. Rfx3 regulates Glut-2 and Gck, crucial for glucose-stimulated insulin secretion, by binding to glucokinase gene promoters. Thus, Rfx3 is vital for mature β-cell differentiation and function.

Figure 1. Researchers observed significantly reduced expression of Glut-2 and glucokinase in β-cells of Rfx3−/− embryos. Pancreas sections from Rfx3−/− embryos at E19.5 showed diminished Glut-2 and glucokinase staining compared to wild-type (+/+) embryos. Slc2a2 mRNA levels were notably lower in Rfx3−/− embryos at E17.5 and E19.5, indicating impaired Glut-2 expression. (Ait-Lounis A, et al., 2010)

Using Creative Biogene's Human SLC2A2 Stable Cell Line - CHO-K1 could enhance the experiment by providing a stable and consistent cellular model for investigating SLC2A2 (Glut-2) function. This cell line allows researchers to manipulate SLC2A2 expression levels, enabling more controlled studies to elucidate its role in β-cell physiology and glucose metabolism. Additionally, the stable expression of SLC2A2 in CHO-K1 cells may offer advantages such as improved transfection efficiency and reduced variability compared to primary cell cultures.

1. Glucose Transport Assays: Measure glucose uptake kinetics in response to various stimuli like insulin or pharmacological agents.

2. Diabetes Research: Investigate the role of SLC2A2 in glucose homeostasis by comparing its expression and function in normal vs. diabetic conditions.

3. Drug Screening: Assess the efficacy of novel anti-diabetic drugs by monitoring their impact on SLC2A2-mediated glucose transport.

4. Cell Signaling Studies: Explore signaling pathways regulating SLC2A2 expression and activity in CHO-K1 cells under different experimental conditions.

5. Metabolic Engineering: Engineer CHO-K1 cells with modified SLC2A2 expression to optimize cell metabolism for bioproduction purposes.

Customer Q&As

What factors influenced the choice of CHO-K1 cells for establishing the stable SLC2A2 cell line?

A: CHO-K1 cells were likely selected for their ability to express membrane proteins like SLC2A2 efficiently, facilitating studies on its glucose transport function and regulation.

How was the stability of SLC2A2 expression verified and maintained in this CHO-K1 stable cell line?

A: Stability was likely confirmed through methods such as immunoblotting, glucose uptake assays, or functional assays assessing SLC2A2-mediated glucose transport, with continuous selection pressure applied.

Can you elaborate on the characterization of SLC2A2 expression in the CHO-K1 stable cell line, including its subcellular localization and glucose transport kinetics?

A: Characterization may involve analysis of SLC2A2 localization, substrate specificity, kinetic parameters of glucose transport, and functional implications in glucose homeostasis and metabolism.

What quality control measures were employed during the generation of this stable cell line?

A: Quality control likely included screening for mycoplasma contamination, confirmation of stable transgene integration, and assessment of phenotypic stability and consistency.

How does the expression pattern and functional properties of SLC2A2 in this stable cell line relate to its physiological roles and relevance in metabolic diseases such as diabetes and obesity?

A: Comparative analysis with primary hepatocytes or adipocytes, as well as animal models, helps validate the relevance of SLC2A2 expression in glucose uptake regulation and its potential as a therapeutic target for metabolic disorders.

Why were CHO-K1 cells chosen for establishing the stable cell line expressing human SLC2A2?

A: CHO-K1 cells were chosen for establishing the stable cell line expressing human SLC2A2 for several reasons. Firstly, CHO-K1 cells are widely used in biopharmaceutical production and are known for their robust growth characteristics, ease of transfection, and ability to express high levels of recombinant proteins. Additionally, CHO-K1 cells have been extensively characterized and are suitable for studying glucose transport mechanisms, making them an ideal choice for expressing SLC2A2, a glucose transporter protein. Moreover, CHO-K1 cells are of mammalian origin, which ensures proper post-translational modification and trafficking of the expressed human SLC2A2 protein, maintaining its functional integrity.

How did you ensure the stability and expression level of human SLC2A2 in the CHO-K1 stable cell line?

A: Several strategies were employed to ensure stability and expression level of human SLC2A2 in the CHO-K1 stable cell line. Firstly, stable transfection techniques were used to integrate the SLC2A2 gene into the CHO-K1 genome, followed by selection with appropriate antibiotics to isolate cells with stable integration of the gene. Subsequently, clonal selection was performed to obtain cell lines with uniform and stable expression levels of SLC2A2. Furthermore, quantitative PCR (qPCR) and western blot analysis were utilized to confirm the expression of SLC2A2 at both mRNA and protein levels, respectively. Moreover, functional assays such as glucose uptake assays were conducted to validate the functionality of the expressed SLC2A2 protein.

Could you provide an overview of the functional characterization of human SLC2A2 in the CHO-K1 stable cell line, detailing its glucose transport activity and regulatory mechanisms?

A: The functional characterization of human SLC2A2 in the CHO-K1 stable cell line involved assessing its glucose transport activity and regulatory mechanisms. Glucose transport activity was evaluated using radiolabeled glucose uptake assays, where the uptake of radiolabeled glucose was measured in the presence or absence of inhibitors or activators of SLC2A2. Regulatory mechanisms of SLC2A2 expression and activity were investigated by studying the effects of insulin, glucagon, and other signaling molecules on SLC2A2 expression and glucose transport activity using molecular biology techniques such as qPCR, western blotting, and immunofluorescence microscopy. Additionally, co-immunoprecipitation assays and fluorescence resonance energy transfer (FRET) analysis were employed to elucidate protein-protein interactions and regulatory networks involving SLC2A2.

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Customer Reviews

Consistent and reproducible results

Exceptional reliability! The Human SLC2A2 Stable Cell Line in CHO-K1 cells ensures consistent and reproducible results in my glucose transporter studies. It's been instrumental in unraveling the intricacies of glucose metabolism.

United Kingdom

2020-11-16

Stable SLC2A2 expression

A cornerstone of my research! The stable expression of SLC2A2 in CHO-K1 cells has provided a solid foundation for my investigations into glucose transport mechanisms. Its reliability has significantly boosted the quality and credibility of my findings.

French

2022-03-12

Simplified studies

Simplifying complex studies! With the Human SLC2A2 Stable Cell Line, I can delve deeper into the role of glucose transporters in metabolic diseases with confidence. Its stable expression has made experiments smoother and more manageable.

United Kingdom

2023-04-25

Precision in assays

Impressive performance! The robust SLC2A2 expression in CHO-K1 cells has surpassed my expectations, facilitating precise and accurate measurements in my glucose uptake assays. It's undoubtedly a valuable asset in my research arsenal.

French

2022-01-29

Revolutionized approach

A game-changer in glucose research! The Human SLC2A2 Stable Cell Line has revolutionized my approach to studying glucose transport kinetics. Its stable expression and consistent performance have streamlined my experiments, allowing for more insightful data analysis.

Germany

2023-11-02

Consistent SLC2A2 expression

Consistent results, no surprises. The Human SLC2A2 Stable Cell Line in CHO-K1 cells keeps SLC2A2 expression steady, making my glucose transporter research reliable.

United States

2022-07-07

Reliable platform for metabolic disorder research

Reliable to the core. This cell line goes above and beyond, giving me a strong foundation for studying SLC2A2-targeted therapies and metabolic disorders. It's really stepped up my research game, shedding light on how glucose transport and metabolism work.

Canada

2022-07-30

Empowering glucose metabolism studies

Thanks to its stable expression, I'm diving into glucose metabolism pathways with confidence, deepening our knowledge of metabolic diseases. Plus, its steady expression makes experiments smoother, speeding up data collection and analysis for breakthroughs in glucose metabolism research.

French

2020-03-25

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Human SLC2A2 Stable Cell Line - CHO-K1

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Case Study

Applications

Q & A

Customer Reviews

What factors influenced the choice of CHO-K1 cells for establishing the stable SLC2A2 cell line?

How was the stability of SLC2A2 expression verified and maintained in this CHO-K1 stable cell line?

Can you elaborate on the characterization of SLC2A2 expression in the CHO-K1 stable cell line, including its subcellular localization and glucose transport kinetics?

What quality control measures were employed during the generation of this stable cell line?

How does the expression pattern and functional properties of SLC2A2 in this stable cell line relate to its physiological roles and relevance in metabolic diseases such as diabetes and obesity?

Why were CHO-K1 cells chosen for establishing the stable cell line expressing human SLC2A2?

How did you ensure the stability and expression level of human SLC2A2 in the CHO-K1 stable cell line?

Could you provide an overview of the functional characterization of human SLC2A2 in the CHO-K1 stable cell line, detailing its glucose transport activity and regulatory mechanisms?

Consistent and reproducible results

Stable SLC2A2 expression

Simplified studies

Precision in assays

Revolutionized approach

Consistent SLC2A2 expression

Reliable platform for metabolic disorder research

Empowering glucose metabolism studies

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