Cat.No. : CSC-RO0569 Host Cell: HEK293T
| Cat. No. | CSC-RO0569 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Human SLAMF7. |
| Gene | SLAMF7 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The unique advantages of the Human SLAMF7 Stable Cell Line - HEK293T in immunology research include its stable expression of SLAMF7 (Signaling Lymphocyte Activation Molecule Family 7), which facilitates the study of SLAMF7's role in immune cell activation, proliferation, and differentiation. Additionally, the HEK293T cell line, known for its high transfection efficiency and good protein expression capabilities, makes it an ideal platform for studying SLAMF7 signaling pathways and related immune responses. This cell line can also be used for high-throughput screening studies to identify small molecules or biologics that modulate SLAMF7 function.
A: When developing targeted drugs for the Human SLAMF7 Stable Cell Line - HEK293T cell line, researchers need to consider key factors such as the expression level of SLAMF7 on the cell surface, the signaling mechanisms of SLAMF7, and the impact of drugs on cell function. Additionally, the cytotoxicity, selectivity, and pharmacological properties at the cellular level of the drugs need to be evaluated. Researchers should also consider the stability, bioavailability, and pharmacokinetics of the drugs, as well as potential off-target effects and side effects.
A: The potential application of the Human SLAMF7 Stable Cell Line - HEK293T cell line in vaccine development is very broad. Since SLAMF7 plays a key role in immune cell activation, this cell line can be used to study how vaccines enhance immune responses through the SLAMF7 pathway. Furthermore, by screening vaccine candidates that can activate SLAMF7 in this cell line, the vaccine development process can be accelerated. This cell line can also be used to evaluate the safety and efficacy of vaccines, especially in simulating human immune responses.
A: The common challenges when using the Human SLAMF7 Stable Cell Line - HEK293T cell line for gene editing include improving gene editing efficiency, ensuring specificity of editing, and maintaining the stability of the cell phenotype after editing. Solutions may include optimizing the design of the CRISPR/Cas9 system, using more efficient transfection methods, and developing new gene editing tools such as base editors. Additionally, a rigorous screening and validation process can ensure the reliability of the edited cell line in subsequent research.
A: The Human SLAMF7 Stable Cell Line - HEK293T cell line plays a crucial role in studying SLAMF7-mediated cell-to-cell interactions. This cell line provides a stable platform for investigating how SLAMF7 affects interactions between immune cells, including their adhesion, activation, and signal transduction. Researchers can explore the function of SLAMF7 in the immune cell network and how it participates in regulating immune responses using this cell line.
A: The Human SLAMF7 Stable Cell Line - HEK293T cell line can be used to study the role of SLAMF7 in immune cell development by simulating the differentiation and maturation processes of immune cells. By modulating the expression levels of SLAMF7, researchers can observe changes in the immune cell phenotype and function.
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