Cat.No. : CSC-RO0473 Host Cell: CHO-K1
| Cat. No. | CSC-RO0473 |
| Description | This cell line is derived from CHO-K1 and is engineered to stably overexpress Human SIGLEC10. |
| Gene | SIGLEC10 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
SIGLEC10's critical function in gastric cancer (GC) is highlighted by the researchers through an examination of its effects on CD8 T cells that have invaded tumors. Through in vitro tests, they demonstrate that SIGLEC10, which is expressed on CD68 macrophages, blocks the Akt/P38/Erk signaling pathway, which in turn reduces CD8 T cell activity and proliferation. Furthermore, SIGLEC10 inhibition improves CD8 T cell effector activity in both in vivo and ex vivo settings. Significantly, a poor prognosis for GC is linked to SIGLEC10 macrophages. These results highlight SIGLEC10's role as a direct inhibitor of T cell function, pointing to possible uses for it as an immunotherapeutic target and GC prognostic marker.
Figure 1. Using a pHR lentiviral vector, the researchers cloned SIGLEC10 to produce overexpressing cell lines. Lentivoresis was observed when PHR-SIGLEC10-SFFV-IRES-EGFP was co-transfected into HEK293T cells along with additional plasmids. After infecting THP-1 cells with these, the cells were centrifuged and incubated to aid in the transfer of genes. The consequences of SIGLEC10 overexpression can be studied thanks to this technique. (Guo Y, et al., 2023)
A: Our human SIGLEC10 stable cell line has undergone rigorous stability testing and maintains stable expression levels over multiple passages. We regularly validate cell lines and record cell line stability data. Customers can request this data from us to understand the stability performance of our products.
A: Our human SIGLEC10 stable cell line is fully functional and widely used in research related to immune regulation. We provide detailed functional verification data, including cell signaling pathway analysis, apoptosis experiments, etc. Customers can refer to these data to evaluate the performance of our products.
A: Although our human SIGLEC10 stable cell line is mainly used for in vitro research, we can also provide relevant in vivo research data support, proving the application potential of our products in in vivo models. Customers can refer to this data to understand the performance and range of applications of our products in in vivo studies.
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In terms of gene expression, the expression of SIGLEC10 is very stable, which is very important for studying the function and signaling mechanism of cell surface glycoproteins. The morphology of the cells is good and there are no abnormal changes, and the accuracy of the experimental data is guaranteed. CHO-K1 cells are used for their excellent protein expression capabilities and ease of operation.
The gene expression of SIGLEC10 is stable, ensuring the reliability of research data. The platform provides detailed instructions and technical support, and the entire user experience is very professional and satisfying. Whether it is product quality or service level, this product is highly recommended.
While conducting research using the Human SIGLEC10 Stable Cell Line - CHO-K1, I found that this cell line is ideal for studying glycoprotein function in the innate immune system. The cells grew rapidly in the culture medium and maintained good shape and vitality. Overall, this cell line performs well in research applications and is an ideal tool for further research.
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