Human SDCBP Knockout Cell Line-Hela

Name: Human SDCBP Knockout Cell Line-Hela
SKU: CSC-RT2720
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RT2720

Target Gene : SDCBP Host Cell : HeLa

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RT2720
Description	This cell is a stable cell line with a homozygous knockout of human SDCBP using CRISPR/Cas9.
Target Gene	SDCBP
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Size	1 vial (>10^6 cell/vial)
Validation	Sequencing
Storage	Liquid nirtogen
Shipping	Dry ice package

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:4-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	SDCBP syndecan binding protein (syntenin) [ Homo sapiens ]
Gene Symbol	SDCBP
Synonyms	ST1; SYCL; MDA-9; TACIP18
Gene Description	syndecan binding protein (syntenin)
GeneID	6386
Uni ProtID	O00560
mRNA Refseq	NM_001007069.1
Protein Refseq	NP_001007070.1
Chromosome Location	8q12
Function	cell adhesion molecule binding; cytoskeletal adaptor activity; ephrin receptor binding; frizzled binding; glycoprotein binding; growth factor binding; interleukin-5 receptor binding; protein C-terminus binding; protein N-terminus binding; protein heterodimerization activity; protein homodimerization activity; syndecan binding; syndecan binding;
Pathway	Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; IL-5 Signaling Pathway, organism-specific biosystem; L1CAM interactions, organism-specific biosystem; Neurofascin interactions, organism-specific biosystem; Syndecan-1-mediated signaling events, organism-specific biosystem; Syndecan-2-mediated signaling events, organism-specific biosystem;
MIM	602217

Quick Inquiry

Background

Applications

Q & A

Customer Reviews

The SDCBP gene encodes a protein called syntenin-1, which is present in humans and plays a crucial role in cellular functions. One of the main functions of syntenin-1 involves organizing the cytoskeleton and membrane structure, which is essential for maintaining cell shape, stability, and intracellular trafficking. The interaction of this protein with adherens junctions and focal adhesions emphasizes its role in cell adhesion, which is essential for tissue integrity and signal transduction. In addition, syntenin-1 also affects the activation of transcription factors, affecting gene expression and cellular responses.

The SDCBP gene exhibits alternative splicing, resulting in multiple transcript variants encoding different isoforms of syntenin-1. Its expression has been found in various tissues, including the retinal pigment epithelium, visceral pleura, amniotic fluid, and skin. Notably, syntenin-1 interacts with several important proteins, such as EFNB1, GRIK1, GRIK2, Merlin, and TRAF6, among others. These interactions highlight its involvement in a wide range of signaling pathways and cellular mechanisms, such as positive regulation of transforming growth factor-β receptor signaling, intracellular signal transduction, and organization of the actin cytoskeleton.

The Human SDCBP Knockout Cell Line-Hela is a powerful tool to advance research in a variety of biomedical fields. The following are the main applications of this cell line:

1. Cancer Research: The Human SDCBP Knockout Cell Line-Hela provides a valuable model for studying the role of SDCBP (syndecan-binding protein) in cancer progression and metastasis. Since SDCBP is involved in cell adhesion, migration, and signaling pathways, knocking out this gene in Hela cells helps to elucidate its contribution to tumor biology.

2. Drug Screening: This knockout cell line can be used for high-throughput drug screening to identify potential therapeutic compounds that target pathways affected by SDCBP loss. By comparing the responses between wild-type and knockout cells, researchers can identify drugs that specifically modulate the relevant cellular mechanisms.

3. Signal Transduction Studies: SDCBP is known to interact with a variety of signaling molecules. Knockout cell lines serve as models for more precise studies of these signaling pathways, allowing scientists to understand how the loss of SDCBP affects signal transduction and downstream effects.

4. Gene Function Analysis: The human SDCBP knockout cell line-Hela is ideal for basic studies of gene function. Researchers can observe phenotypic changes due to SDCBP knockout, gaining insight into its physiological role and interactions with other cellular proteins.

5. Biomarker Discovery: By utilizing this cell line in proteomic and genomic studies, new biomarkers associated with the SDCBP pathway can be identified.

Customer Q&As

What is the recommended growth medium? Does it require antibiotic selection?

A: DMEM supplemented with 10% fetal bovine serum. <br> It is not required to add the selection antibiotics when culturing the KO cells.

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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Customer Reviews

Good experimental results

By studying SDCBP knockout cells, we can assess the impact of its absence on signaling pathways like the mitogen-activated protein kinases (MAPK) or phosphatidylinositol-3 kinase (PI3K) pathways. Good experimental results were obtained.

United States

2021-05-12

Helpful

SDCBP knockout cells allow us to investigate the impact of its absence on disease-related pathways and mechanisms, offering potential novel therapeutic targets for neurodegenerative diseases. These cell lines are very helpful for our research.

United States

2021-08-15

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Human SDCBP Knockout Cell Line-Hela

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

What is the recommended growth medium? Does it require antibiotic selection?

How is the knockout cell line validated?

Is the product a single clonal cell or mixed cell pool?

Can I confirm gene knockout by RT-qPCR?

How can I store the cell product?

Is it possible to get multiple knockout clones for my GOI?

Good experimental results

Helpful

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