Cat.No. : CSC-SC009066-1 Host Cell: HEK293T
| Cat. No. | CSC-SC009066-1 |
| Description | This cell line is engineered to stably express human mucosal vascular addressin cell adhesion molecule 1 (MADCAM1) in HEK293 cells. |
| Gene | MADCAM1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Integrins are important for cell adhesion, migration, and tissue formation, especially in inflammatory reactions and immune cell trafficking. Mutations in integrin β7, particularly α4β7, have been studied for their impact on lymphocyte homing to gut-associated lymphoid tissue (GALT). Agonist-induced activation, which is necessary for lymphocyte rolling and arrest, is impaired by mutations, according to research. For instance, the proline mutation within the integrin's transmembrane domain disrupts its structural integrity, leading to decreased integrin activation and lymphocyte homing.
Figure 1. The researchers utilized Jurkat T cells and CRISPR/Cas9 mutagenesis to study talin's role in activating integrin α4β7. They examined the expression of talin and β7, assessed binding to MAdCAM-1, and evaluated adhesion under shear stress conditions to understand the integrin's functionality. (Sun H, et al., 2018)
Creative Biogene's Human MADCAM1 Stable Cell Line - HEK293 is engineered for applications in integrin-related research, providing a reliable platform to study MAdCAM-1 interactions and integrin activation mechanisms. This cell line facilitates the examination of how integrin mutations affect binding and cellular responses, making it invaluable for understanding immune regulation and developing therapeutics targeting inflammatory diseases.
A: Our human MADCAM1 stable cell line has been tested for long-term stability and maintains stable expression levels over multiple passages. We regularly validate cell lines and record cell line stability data. Customers can request this data from us to understand the stability performance of our products.
A: Our human MADCAM1 stable cell lines are fully functional and widely used in studies of intestinal immune regulation. We provide detailed functional validation data, including cell adhesion experiments, immune modulation assays, and more. Customers can refer to these data to evaluate the performance of our products.
A: Our human MADCAM1 stable cell lines have been validated in inflammatory bowel disease research. We provide relevant preclinical research data support to prove the application potential of our products in these fields. Customers can refer to these data to understand the performance and application scope of our products in preclinical studies.
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Recently, I used the Human MADCAM1 Stable Cell Line - HEK293 and the overall experience was very good. The expression of MADCAM1 in this cell line is very stable, especially in its interaction with leukocyte β7 integrins LPAM-1, L-selectin and VLA-4. This cell line has played an important role in research directing leukocytes into mucosal and inflammatory tissues.
Human MADCAM1 Stable Cell Line - HEK293 has brought a lot of convenience to my research. This cell line performed very well in HEK293 cells, especially in terms of interaction with immune cells, and the expression of MADCAM1 was very stable and consistent. The high transfection efficiency and easy operation of HEK293 cells made my experiment smoother. The service provided by the supply platform is also very considerate and professional from purchase to technical support, which greatly improved my experimental efficiency.
MADCAM1's interaction with leukocyte integrins and its guidance function in mucosal and inflammatory tissues provided important data for my research. The high transfection efficiency and rapid growth of HEK293 cells also make the experiment smoother. The supplier's efficient service and professional support greatly helped me with my research.
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