Cat.No. : CSC-RO0684 Host Cell: Nme
| Cat. No. | CSC-RO0684 |
| Description | This cell line is engineered to stably co-overexpress human HRAS bearing G12V mutation and human FAM3C with 2A-linked EGFP. |
| Gene | HRAS-G12V/FAM3C-2A-EGFP |
| Gene Species | Homo sapiens (Human) |
| Host Cell | Nme |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human HRAS-G12V/FAM3C-2A-EGFP Stable Cell Line - Nme is constructed by stably expressing the HRAS-G12V mutant and the FAM3C-2A-EGFP fusion protein in Nme cells. This cell line enables researchers to observe and analyze the impact of HRAS gene mutations on cell growth and signaling, with the expression of EGFP making the localization and functional study of proteins within the cells more intuitive.
A: The application of this cell line in cancer research focuses on the relationship between HRAS gene mutations and tumorigenesis, development, and therapeutic resistance. By studying the role of the HRAS-G12V mutant in Nme cells, scientists can better understand how this mutation promotes tumor cell proliferation and invasion, providing a theoretical basis for the development of new anticancer strategies.
A: This cell line can serve as a screening platform to identify and test potential drugs targeting the HRAS-G12V mutant. By observing the expression and localization changes of the EGFP-tagged protein after drug treatment, researchers can evaluate the inhibitory effects of drugs on the HRAS signaling pathway, thereby screening for effective anticancer drug candidates.
A: By stably expressing the HRAS-G12V mutant, this cell line provides a model for studying the role of the Ras/MAPK signaling pathway within cells. Researchers can use this cell line to explore how HRAS mutations affect the signaling network and how these changes lead to alterations in cell behavior, which is crucial for understanding the molecular mechanisms of cancer and other Ras-related diseases.
A: This cell line can help researchers study how the HRAS-G12V mutation affects cell cycle regulation. By observing the dynamic changes of the EGFP-tagged protein during different phases of the cell cycle, scientists can reveal how HRAS mutations impact the expression and activity of cell cycle proteins, which is significant for understanding the mechanisms of tumor cell proliferation.
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The Human HRAS-G12V/FAM3C-2A-EGFP Stable Cell Line - Nme is a vital tool for studying the role of HRAS mutations in cancer development.
The EGFP tag allows for the visualization of HRAS-G12V protein localization and dynamics within the cell, facilitating observation and analysis.
Provides a platform for screening potential drug candidates targeting HRAS mutations.
Offers a stable cell model for studying HRAS-related diseases, contributing to the elucidation of disease mechanisms.
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