Human HRAS-G12V/FAM3C-2A-EGFP Stable Cell Line - Nme

The HRAS (Harvey Rat Sarcoma Viral Oncogene Homolog) gene encodes a small GTPase protein that is involved in cell growth, differentiation, and survival. The G12V mutation in HRAS is one of the most common oncogenic mutations, leading to constitutive activation of the protein and uncontrolled cell proliferation. FAM3C, or Cytokine-like protein 1, is a protein that has been implicated in various cellular processes, including apoptosis and cell cycle regulation. The 2A peptide and EGFP (enhanced green fluorescent protein) are used to facilitate the expression and visualization of the proteins within the cell.

The Nme cell line has been engineered to stably co-express the HRAS-G12V mutation along with FAM3C and EGFP. This stable cell line is a powerful tool for studying the effects of the HRAS-G12V mutation on cellular behavior, particularly in the context of oncogenic transformation. Researchers can use this cell line to investigate the molecular mechanisms by which HRAS-G12V drives cancer progression and to identify potential therapeutic targets. The inclusion of EGFP allows for the tracking of protein expression and localization, providing a visual readout of the cell's response to various experimental conditions.