Human HRAS-G12V/EGFP Stable Cell Line - Nme

Name: Human HRAS-G12V/EGFP Stable Cell Line - Nme
SKU: CSC-RO0683
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0683

Host Cell : Nme Size : >1x10⁶ frozen cells/vial

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Cat. No.	CSC-RO0683
Description	This cell line is engineered to stably co-overexpress EGFP and human HRAS bearing G12V mutation.
Target Gene	HRAS-G12V/EGFP
Gene Species	Homo sapiens (Human)
Host Cell	Nme
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

CSC-RO0683-Human HRAS-G12V/EGFP Stable Cell Line - Nme-Datasheet

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Background

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The Human HRAS-G12V/EGFP Stable Cell Line is a cell line that has been engineered to express a mutated form of the HRAS gene, specifically the G12V mutation, fused with the EGFP (enhanced green fluorescent protein). HRAS is a small GTPase that is involved in cell growth, differentiation, and survival. Mutations in HRAS, such as the G12V mutation, are commonly found in various cancers and are associated with constitutive activation of the protein, leading to uncontrolled cell proliferation. The inclusion of EGFP allows for the visualization of cells expressing the HRAS mutation, which is useful for studying the effects of this mutation on cell behavior and for screening potential therapeutic agents. This cell line is particularly valuable for research into the mechanisms of HRAS-mediated transformation and for the development of targeted therapies against HRAS-driven cancers.

The Human HRAS-G12V/EGFP Stable Cell Line - Nme is a specialized tool for studying the effects of the HRAS G12V mutation, which is commonly associated with cancer. This cell line has several unique applications:

(1) Cancer Biology Research: The G12V mutation in HRAS leads to constitutive activation of the protein, promoting uncontrolled cell growth. This cell line allows researchers to study the cellular changes induced by this mutation, including alterations in cell cycle regulation and signal transduction pathways.

(2) Drug Discovery: The EGFP tag in this cell line enables the visualization of cells expressing the mutant HRAS. This feature can be leveraged for high-throughput screening of compounds that may inhibit HRAS activity or reverse its oncogenic effects, aiding in the discovery of new cancer treatments.

(3) Mechanism of Action Studies: By expressing the HRAS G12V mutation, this cell line can be used to investigate the molecular mechanisms by which HRAS mutations contribute to cancer development and progression. This includes studying the interaction of HRAS with other proteins and the downstream effects on gene expression and cellular behavior.

Customer Q&As

How can the Human HRAS-G12V/EGFP Stable Cell Line - Nme be used to study the relationship between HRAS mutations and cell signaling?

A: This cell line, by expressing the HRAS gene with the G12V mutation, helps researchers observe how this mutation affects intracellular signaling pathways, particularly those related to cell proliferation and survival, such as the MAPK and PI3K/AKT/mTOR pathways.

How can the Human HRAS-G12V/EGFP Stable Cell Line - Nme be used in drug development to assess the efficacy of potential drugs?

A: By monitoring the fluorescence intensity of EGFP, researchers can evaluate the effects of drugs on the activity of HRAS G12V mutant cells, thus screening for candidate drugs that may inhibit or activate the HRAS signaling pathway.

What role does the Human HRAS-G12V/EGFP Stable Cell Line - Nme play in studying the relationship between HRAS mutations and cancer development?

A: This cell line provides a model for studying how HRAS G12V mutations promote the occurrence and development of cancer, including the proliferation, invasion, and metastasis of tumor cells.

How can the Human HRAS-G12V/EGFP Stable Cell Line - Nme be used to study the impact of HRAS mutations on cell cycle regulation?

A: By analyzing the cell cycle distribution of EGFP-tagged HRAS G12V cells, researchers can understand how HRAS mutations affect the cell cycle progression, especially at the G1/S and G2/M checkpoints.

How can the Human HRAS-G12V/EGFP Stable Cell Line - Nme be used to study the role of HRAS mutations in cell differentiation?

A: By observing the phenotypic changes of HRAS G12V mutant cells treated with specific differentiation inducers, researchers can explore how HRAS mutations affect the differentiation potential of cells.

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Customer Reviews

Mutation Research Tool

The Human HRAS-G12V/EGFP Stable Cell Line - Nme provides a model for studying the effects of the HRAS G12V mutation on cell functions.

United Kingdom

2021-02-13

Live Imaging

The EGFP tag allows researchers to observe the expression and localization of the HRAS G12V protein in real-time within cells.

United Kingdom

2020-02-21

Cancer Model

This cell line serves as a powerful tool for studying the role of HRAS mutations in cancer development.

Germany

2021-01-11

Signaling Analysis

Aids in studying how the HRAS G12V mutation affects intracellular signaling pathways.

United Kingdom

2021-10-26

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Human HRAS-G12V/EGFP Stable Cell Line - Nme

Cell Line Information

Cell Culture Information

Safety and Packaging

Documents

Background

Applications

Q & A

Customer Reviews

How can the Human HRAS-G12V/EGFP Stable Cell Line - Nme be used to study the relationship between HRAS mutations and cell signaling?

How can the Human HRAS-G12V/EGFP Stable Cell Line - Nme be used in drug development to assess the efficacy of potential drugs?

What role does the Human HRAS-G12V/EGFP Stable Cell Line - Nme play in studying the relationship between HRAS mutations and cancer development?

How can the Human HRAS-G12V/EGFP Stable Cell Line - Nme be used to study the impact of HRAS mutations on cell cycle regulation?

How can the Human HRAS-G12V/EGFP Stable Cell Line - Nme be used to study the role of HRAS mutations in cell differentiation?

Mutation Research Tool

Live Imaging

Cancer Model

Signaling Analysis

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