Cat.No. : CSC-RO0683 Host Cell: Nme
| Cat. No. | CSC-RO0683 |
| Description | This cell line is engineered to stably co-overexpress EGFP and human HRAS bearing G12V mutation. |
| Gene | HRAS-G12V/EGFP |
| Gene Species | Homo sapiens (Human) |
| Host Cell | Nme |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: This cell line, by expressing the HRAS gene with the G12V mutation, helps researchers observe how this mutation affects intracellular signaling pathways, particularly those related to cell proliferation and survival, such as the MAPK and PI3K/AKT/mTOR pathways.
A: By monitoring the fluorescence intensity of EGFP, researchers can evaluate the effects of drugs on the activity of HRAS G12V mutant cells, thus screening for candidate drugs that may inhibit or activate the HRAS signaling pathway.
A: This cell line provides a model for studying how HRAS G12V mutations promote the occurrence and development of cancer, including the proliferation, invasion, and metastasis of tumor cells.
A: By analyzing the cell cycle distribution of EGFP-tagged HRAS G12V cells, researchers can understand how HRAS mutations affect the cell cycle progression, especially at the G1/S and G2/M checkpoints.
A: By observing the phenotypic changes of HRAS G12V mutant cells treated with specific differentiation inducers, researchers can explore how HRAS mutations affect the differentiation potential of cells.
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The Human HRAS-G12V/EGFP Stable Cell Line - Nme provides a model for studying the effects of the HRAS G12V mutation on cell functions.
The EGFP tag allows researchers to observe the expression and localization of the HRAS G12V protein in real-time within cells.
This cell line serves as a powerful tool for studying the role of HRAS mutations in cancer development.
Aids in studying how the HRAS G12V mutation affects intracellular signaling pathways.
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