Cat.No. : LVIM014Z
Titer: ≥1*10^7 TU/mL / ≥1*10^8 TU/mL / ≥1*10^9 TU/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | LVIM014Z |
| Description | Lentivirus expressing untagged Human HOXB8 under the control of CMV promoter. This lentivirus contains no antibiotic selection marker. |
| Target Gene | HOXB8 |
| Species | Human |
| Titer | Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc. |
| Size | Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. | |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
Previous studies have shown that homeobox 8 (HOXB8) is expressed more in colorectal cancer (CRC) tissues than in normal tissues. However, the exact role of HOXB8 in human CRC cells remains to be elucidated. In this study, researchers established cell lines with stable overexpression and knockdown of HOXB8 by constructing HOXB8 lentiviral expression vectors. The researchers found that overexpression of HOXB8 promoted the proliferation, migration, and invasion of CRC cells, while silencing of HOXB8 produced the opposite effects. HOXB8 knockdown inhibited cell proliferation and invasion in vitro and carcinogenesis and metastasis in vivo. HOXB8 also induced EMT, which is characterized by downregulation of E-cadherin and upregulation of Vimentin, N-cadherin, Twist, Zeb1, and Zeb2. In addition, HOXB8 activated STAT3, which is known to play an oncogenic role in a variety of human malignancies.
While establishing stable overexpression of of HOXB8 in DLD1 cell using HOXB8 lentiviral expression vector (Figure 1a), researchers performed MTT and colony formation assays to evaluate the effects of HOXB8 on the proliferation of CRC cells, and they also evaluated the effect of HOXB8 on the migration and invasion of CRC cells by performing wound healing and transwell assays. The results showed that overexpression of HOXB8 promoted cell proliferation (Figure 1b, c), as well as migration and invasion of CRC cells (Figure 1d, f). These results indicate that HOXB8 has the effect of promoting CRC cell proliferation and invasion in vitro.
Figure 1. HOXB8 increases the proliferation and invasion capacity of CRC cells. (Wang T, et al., 2019)
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