Cat.No. : LVIM017Z
Titer: ≥1*10^7 TU/mL / ≥1*10^8 TU/mL / ≥1*10^9 TU/mL Size: 100 ul/500 ul/1 mL
Storage: -80℃ Shipping: Frozen on dry ice
| Cat. No. | LVIM017Z |
| Description | Lentivirus expressing untagged Human HOXA10 under the control of CMV promoter, and puromycin resistance marker for mammalian cell selection. |
| Target Gene | HOXA10 |
| Species | Human |
| Application | The Human HOXA10 Lentivirus (CMV, Puro) is ideal for various research applications: Cellular Immortalization: Human HOXA10 Lentivirus can be employed to immortalize primary cells, which is invaluable for creating stable cell lines for research and industrial applications. Gene Function Studies: By overexpressing HOXA10, researchers can elucidate its role in various cellular and developmental processes, gaining insights into its function and pathways. Disease Modeling: This lentiviral vector can be used to create disease models, especially for conditions where HOXA10 dysregulation is implicated, such as certain cancers and developmental disorders. Drug Screening: Immortalized cell lines expressing HOXA10 are ideal for high-throughput drug screening and toxicity testing, accelerating drug discovery efforts. |
| Titer | Varies lot by lot, for example, ≥1*10^7 TU/mL, ≥1*10^8 TU/mL, ≥1*10^9 TU/mL etc. |
| Size | Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality lentivirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between lentivirus particle lots. | |
| Mycoplasma | Creative Biogene routinely tests for mycoplasma contamination using a mycoplasma detection kit. Cell lines are maintained for approximately 20 passages before being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks after thawing, cell culture supernatants are tested for mycoplasma contamination. Creative Biogene ensures that lentiviral products are free of mycoplasma contamination. |
| Purity | Creative Biogene evaluates the level of impurities, such as residual host cell DNA or proteins, in prepared lentiviral vectors to ensure they meet quality standards. |
| Sterility | The lentiviral samples were inoculated into cell culture medium for about 5 days and the growth of bacteria and fungi was tested. Creative Biogene ensures that the lentiviral products are free of microbial contamination. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of lentivirus to deliver genetic material into target cells, and assess gene expression and functional activities. |
| Proviral Identity Confirmation | All Creative Biogene lentiviral vectors are confirmed to have correctly integrated provirus using PCR. This test involves transducing cells with serial dilutions of the lentiviral vector, harvesting the cells a few days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion of the expected lentiviral insert. |
A: For the infection process, the supernatant is used to infect the target cells. The supernatant was collected from the viral production stage and based on DMEM media that have been depleted of nutrients at the point of harvest. These characteristics of the supernatant may create a stressful environment for the target cells. DMEM may not be appropriate for the target cells and/or the depleted media is not enough to maintain the target cells. Furthermore, the lentiviral components make the cells more sensitive to cell death.
A: Retrovirus: Classic, can integrate into the genome but with low transduction efficiency. They are useful for gene transfer and protein expression in cells that have low transfection efficiency with other transfection reagents. Lentivirus: Can integrate into the genome with relatively high transduction efficiency and they are very useful for cells that have low transfection efficiency with other transfection reagents. No special competent cells required, as they are stable plasmids. Lentiviruses are a powerful tool for stable gene transfer to both dividing and non-dividing cells in vitro and in vivo. Adenovirus: Only work transiently (about 7 days) but have almost 100% transduction efficiency. Adenoviruses can infect a broad range of cell types with the highest efficiency and infection is not dependent on active host cell division. A second key feature is that high virus titers and high-level gene expression can be obtained in most mammalian cells.
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I trust Creative Biogene's pre-made lentivirus products. This is because its lentiviral expression structure is validated by full-length sequencing, restriction digestion and PCR size using gene-specific and vector-specific primers. The products are proven to be free of bacterial, fungal and common mycoplasma contamination.
Creative Biogene offers particles containing different antibiotic markers that allow for easy transduction assays and screening.
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