Cat.No. : CSC-RO0375 Host Cell: Ba/F3
Size: >1x10^6 frozen cells/vial, 1 mL Stability: Stable in culture over a minimum of 10 passages
| Cat. No. | CSC-RO0375 |
| Description | This cell line is engineered to stably overexpress exogenous human FGFR2-BICC1 fusion protein. |
| Target Gene | FGFR2-BICC1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | Ba/F3 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Growth Conditions | 37 °C, 5% CO2 |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Size | >1x10^6 frozen cells/vial, 1 mL |
| Biosafety Level | 2 |
| Thawing & Subculturing Instructions | 1. Thaw cells by gently swirling in a 37°C water bath. To limit contamination, do not submerge the O-ring and cap. 2. When cells are ~70% thawed (~1 min), transfer the vial into a biosafety cabinet, and wipe the surface with 70% ethanol. Allow tube to dry completely. 3. Transfer the cells gently into a 15 mL conical tube containing 10 mL of pre-warmed culture medium (without antibiotic selection marker). Centrifuge cells at ~125 x g for 5~7 min. 4. Remove supernatant without disturbing the pellet, and resuspend cells in 1 mL culture medium (without antibiotic selection marker). Transfer cells to a 6-well plate containing ~2 mL pre-warmed growth medium (without antibiotic selection marker) or a T25 flask containing 5 mL pre-warmed culture medium (without antibiotic selection marker). 5. Incubate the culture at 37°C with 5% CO2. 6. Subculture: split saturated culture 1:4 ~ 1:6 every 3 days; seed out at about 1~3 x 10^5 cells/mL. |
| Growth Properties | Suspension, round |
| Freeze Medium | Frozen with 70% medium, 20% FBS, 10% DMSO |
| Freezing Instructions | Cells are recommended to generate additional frozen stocks at early passages. Frozen stocks should be preserved in a designated cryopreservation medium or in 70% RPMI 1640 + 20% FBS + 10% DMSO (without antibiotic selection marker). 1. Prepare the freezing medium (70% RPMI 1640 + 20% FBS + 10% DMSO, without antibiotic selection marker) fresh immediately before use. 2. Keep the freezing medium on ice and label cryovials. 3. Transfer cells to a sterile, conical centrifuge tube, and count the cells. 4. Centrifuge the cells at 250 x g for 5 minutes at room temperature and carefully aspirate off the medium. 5. Resuspend the cells at a density of at least 3 x10^6 cells/ml in chilled freezing medium. 6. Aliquot 1 ml of the cell suspension into each cryovial. 7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer. 8. Transfer vials to liquid nitrogen for long-term storage. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: This cell line provides a model system to study the FGFR2-BICC1 fusion gene's contribution to oncogenic signaling pathways. It allows for the assessment of downstream effects on cell proliferation, survival, and differentiation, which are key processes in tumorigenesis.
A: By utilizing this cell line, researchers can test the efficacy of targeted therapies against the FGFR2-BICC1 fusion, such as FGFR inhibitors, which may lead to personalized treatment strategies for patients harboring this genetic alteration.
A: Western blotting can be used to validate the expression of the fusion protein, while functional assays, such as phosphorylation assays, can determine its kinase activity. Additionally, luciferase reporter assays may be utilized to study the transcriptional activity regulated by the FGFR2-BICC1 signaling.
A: Absolutely. This cell line can serve as a platform for long-term drug treatment studies to identify potential mechanisms by which cancer cells may develop resistance to FGFR2 inhibitors, which is critical for the development of second-line therapies.
A: The cell line could be used in high-throughput assays to identify compounds that inhibit the kinase activity of the fusion protein or its downstream signaling. This could accelerate the discovery of new drugs that specifically target the molecular abnormalities associated with the FGFR2-BICC1 fusion.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The Human FGFR2-BICC1 Stable Cell Line - BaF3 has been engineered to overexpress the FGFR2-BICC1 fusion gene consistently. This results in a stable and reproducible platform that is crucial for studying the biochemical and physiological functions of this gene fusion, making it particularly valuable for research into signal transduction pathways where FGFR2-BICC1 is implicated.
Consistency is key in scientific experiments. The Human FGFR2-BICC1 Stable Cell Line - BaF3 offers robust reproducibility, ensuring that experiments can be repeated with the same conditions leading to comparable results. This stability is beneficial for longitudinal studies requiring repeated measurements under consistent conditions.
This cell line comes in a format that is easy to culture and maintain, reducing the need for extensive technical training. The Human FGFR2-BICC1 Stable Cell Line - BaF3 allows us to focus more on their experimental designs and less on cell preparation, streamlining the research process.
Using the Human FGFR2-BICC1 Stable Cell Line - BaF3 can significantly cut down on the time typically required for cell line development and genetic engineering. We can bypass these initial steps and proceed directly to their experimental investigations, which is particularly advantageous in fast-paced research environments where time is critical.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
