Cat.No. : CSC-RO0368 Host Cell: Ba/F3
Size: >1x10^6 frozen cells/vial, 1 mL Stability: Stable in culture over a minimum of 10 passages
| Cat. No. | CSC-RO0368 |
| Description | This cell line is engineered to stably overexpress exogenous human ETV6-SYK fusion protein. |
| Target Gene | ETV6-SYK |
| Gene Species | Homo sapiens (Human) |
| Host Cell | Ba/F3 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Growth Conditions | 37 °C, 5% CO2 |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Size | >1x10^6 frozen cells/vial, 1 mL |
| Biosafety Level | 2 |
| Thawing & Subculturing Instructions | 1. Thaw cells by gently swirling in a 37°C water bath. To limit contamination, do not submerge the O-ring and cap. 2. When cells are ~70% thawed (~1 min), transfer the vial into a biosafety cabinet, and wipe the surface with 70% ethanol. Allow tube to dry completely. 3. Transfer the cells gently into a 15 mL conical tube containing 10 mL of pre-warmed culture medium (without antibiotic selection marker). Centrifuge cells at ~125 x g for 5~7 min. 4. Remove supernatant without disturbing the pellet, and resuspend cells in 1 mL culture medium (without antibiotic selection marker). Transfer cells to a 6-well plate containing ~2 mL pre-warmed growth medium (without antibiotic selection marker) or a T25 flask containing 5 mL pre-warmed culture medium (without antibiotic selection marker). 5. Incubate the culture at 37°C with 5% CO2. 6. Subculture: split saturated culture 1:4 ~ 1:6 every 3 days; seed out at about 1~3 x 10^5 cells/mL. |
| Growth Properties | Suspension, round |
| Freeze Medium | Frozen with 70% medium, 20% FBS, 10% DMSO |
| Freezing Instructions | Cells are recommended to generate additional frozen stocks at early passages. Frozen stocks should be preserved in a designated cryopreservation medium or in 70% RPMI 1640 + 20% FBS + 10% DMSO (without antibiotic selection marker). 1. Prepare the freezing medium (70% RPMI 1640 + 20% FBS + 10% DMSO, without antibiotic selection marker) fresh immediately before use. 2. Keep the freezing medium on ice and label cryovials. 3. Transfer cells to a sterile, conical centrifuge tube, and count the cells. 4. Centrifuge the cells at 250 x g for 5 minutes at room temperature and carefully aspirate off the medium. 5. Resuspend the cells at a density of at least 3 x10^6 cells/ml in chilled freezing medium. 6. Aliquot 1 ml of the cell suspension into each cryovial. 7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer. 8. Transfer vials to liquid nitrogen for long-term storage. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The ETV6-SYK gene represents a fusion oncogene that arises from the fusion of the ETV6 (ETS variant 6) and SYK (spleen tyrosine kinase) genes. This fusion is commonly detected in certain hematopoietic malignancies, particularly acute lymphoblastic leukemia (ALL) and sometimes in myelodysplastic syndromes. It is considered a driver of leukemogenesis, contributing to uncontrolled cell growth and survival.
A: The ETV6-SYK fusion gene contributes to leukemogenesis by activating aberrant signaling pathways. The fusion event results in the constitutive activation of the SYK kinase domain, leading to uncontrolled cell proliferation, inhibition of apoptosis, and leukemic transformation. This fusion is known to dysregulate normal hematopoiesis and lead to the development of leukemia.
A: Targeting the ETV6-SYK fusion gene has therapeutic implications for the treatment of hematopoietic malignancies. Specifically, targeted therapies such as tyrosine kinase inhibitors (TKIs) and other drugs that can inhibit the signaling pathways activated by the fusion protein are being explored. These treatments aim to slow or stop the progression of leukemia cells with ETV6-SYK rearrangements.
A: The ETV6-SYK fusion gene is detected through a variety of molecular diagnostic methods, including reverse transcription polymerase chain reaction (RT-PCR), fluorescence in situ hybridization (FISH), and next-generation sequencing (NGS). These techniques can identify the specific fusion event, allowing for accurate diagnosis and prognosis of patients with hematopoietic malignancies associated with this fusion gene.
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The Human ETV6-SYK Stable Cell Line - BaF3 is securely delivered using an efficient and reliable system, ensuring the integrity of the cells during transit and minimizing the risk of damage or contamination.
The cell line is shipped and stored under optimized conditions, including proper temperature and handling, to maintain cell viability and functionality, allowing researchers to receive the cells in optimal condition for their experiments.
The Human ETV6-SYK Stable Cell Line - BaF3 has a wide range of applications in cancer research, particularly in studying the pathogenesis and developing potential therapies for ETV6-SYK-related hematological malignancies.
The Human ETV6-SYK Stable Cell Line - BaF3 complies with all legal and intellectual property requirements, ensuring that researchers can use the cell line confidently, knowing that they are adhering to ethical and legal standards in their work.
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