Human ENTPD1 Stable Cell Line-HEK293T

The ENTPD1 gene, also referred to as CD39 or NTPDase1, encodes ectonucleoside triphosphate diphosphohydrolase 1, a plasma membrane-bound enzyme that hydrolyzes extracellular ATP and ADP into AMP. This protein plays a central role in purinergic signaling by regulating the levels of extracellular nucleotides and nucleotide-derived metabolites. The Human ENTPD1 Stable Cell Line-HEK293T is generated by stably transfecting the human ENTPD1 gene into the HEK293T cell line, a derivative of the human embryonic kidney 293 line that expresses the SV40 large T antigen. These cells constitutively express functional ENTPD1 on the cell surface, providing a reliable and reproducible in vitro system for studying the biochemical and pharmacological properties of this ectonucleotidase without the need for repeated transfection or transient expression.

This cell line is widely used in purinergic signaling research, particularly for investigating the hydrolysis of extracellular nucleotides and the subsequent generation of adenosine via the CD39/CD73 pathway. It serves as a valuable tool for screening small molecule inhibitors or modulators of ENTPD1 activity, which are of growing interest in cancer immunotherapy and inflammatory disease research. The stable expression system also supports studies on the role of CD39 in immune regulation, including its function on regulatory T cells and its impact on the tumor microenvironment. Additionally, researchers use this line for downstream applications such as enzyme activity assays, flow cytometry analysis of surface CD39 expression, and co-culture experiments to examine the effects of ENTPD1-mediated nucleotide depletion on immune cell function. The consistent and long-term expression of ENTPD1 in this stable line makes it a practical alternative to primary cells or transient systems for both basic mechanistic studies and drug discovery efforts.