Cat.No. : CSC-RO1051 Host Cell: HEK293T
| Cat. No. | CSC-RO1051 |
| Description | HEK293-ENTPD1 cell line is engineered to stably overexpress human ENTPD1. |
| Gene | ENTPD1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: When using the Human ENTPD1 Stable Cell Line-HEK293 in research involving human subjects or clinical applications, researchers must adhere to ethical guidelines, obtain appropriate informed consent when working with patient-derived samples, and ensure compliance with institutional review board (IRB) or ethics committee requirements. Additionally, ethical considerations related to genetic manipulation and potential therapeutic applications should be carefully evaluated.
A: The Human ENTPD1 Stable Cell Line-HEK293 originates from human embryonic kidney 293 (HEK293) cells that were transfected with a stable expression construct containing the human ENTPD1 (Ectonucleoside triphosphate diphosphohydrolase 1) gene. This gene encodes for CD39, an enzyme involved in purinergic signaling and immune responses. The cell line was established through a transfection and selection process to create stable cell populations expressing human ENTPD1.
A: CD39 (ENTPD1) plays a critical role in purinergic signaling by hydrolyzing extracellular ATP and ADP into AMP. This enzymatic activity leads to the attenuation of purinergic receptor activation, specifically P2X and P2Y receptors. In the Human ENTPD1 Stable Cell Line-HEK293, CD39 expression can be studied to understand its impact on purinergic signaling and immune cell activation.
A: One potential challenge is that the Human ENTPD1 Stable Cell Line-HEK293 represents a simplified model of purinergic signaling, and its behavior may not fully mimic the complexity of in vivo immune responses. Additionally, researchers should ensure that the expression and function of CD39 (ENTPD1) are validated and consistent for reliable experimental results.
A: CD39 (ENTPD1) is known to interact with other molecules involved in the immune system, such as CD73 (another ectonucleotidase) and various purinergic receptors. These interactions can modulate the effects of adenosine, which has immunosuppressive properties. Targeting CD39 in immunotherapy has been explored to enhance anti-tumor immune responses, making it an attractive target for drug development.
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