Cat.No. : CSC-DC004785-1
Host Cell: HEK293 (Hela and other cell types are also available) Validation: Real-Time RCR
| Cat. No. | CSC-DC004785-1 |
| Description | This cell line is engineered to stably overexpress shRNA targeting human EHMT2. Expression of human EHMT2 gene is stably knocked down in this cell line. |
| Gene | EHMT2 |
| Host Cell | HEK293 (Hela and other cell types are also available) |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | (1) Studying gene functions (2) Studying gene interactions and signaling pathways (3) Target validation and drug discovery (4) Designing diseases models |
| Size Form | >1 × 10^6 cells / vial |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
In embryonal rhabdomyosarcoma (ERMS), Wnt signaling is suppressed, contributing to the inhibition of differentiation. Researchers demonstrate that knockdown of Human EHMT2 promotes myogenic differentiation in ERMS cells by activating canonical Wnt signaling via suppression of the Wnt antagonist DKK1. This effect leads to reduced tumor growth in mouse xenograft models. Mechanistically, EHMT2 regulates Sp1 and p300 enrichment at the DKK1 promoter. Moreover, treatment with EHMT2 inhibitors effectively reduces ERMS cell viability, suggesting potential for EHMT2-targeted therapy in ERMS differentiation.
Figure 1. Researchers demonstrated that Human EHMT2 Knockdown inhibits myoblast differentiation and promotes proliferation. EHMT2 was depleted using siRNA in RD18 cells. Knockdown efficiency was confirmed by western blot analysis. Reduction in H3K9me2 levels was observed after UNC0642 treatment. (Pal A, et al., 2020)
The Human EHMT2 Knockdown Cell Line - HEK293 offered by Creative Biogene enables enhanced investigation in similar experiments by providing a platform to study the effects of EHMT2 knockdown specifically in HEK293 cells. This allows researchers to explore EHMT2's role in cellular processes within a controlled environment, potentially revealing novel insights into its function.
A: HEK293T cells were selected for their high transfection efficiency and suitability for stable transgene expression, crucial for studying ACE2-mediated viral entry and its role in cellular physiology.
A: Stability was likely assessed through methods such as immunoblotting, flow cytometry, or functional assays measuring ACE2-mediated viral entry, with continuous selection pressure applied.
A: Characterization may involve analysis of ACE2 localization, binding kinetics to viral spike proteins, downstream signaling pathways, and functional implications in viral infection, particularly with coronaviruses.
A: Quality control likely included mycoplasma screening, confirmation of stable transgene integration, and assessment of phenotypic stability and consistency.
A: Comparative analysis with primary human cells or relevant animal models helps validate the relevance of ACE2 expression in viral tropism, pathogenesis, and potential therapeutic strategies against viral infections.
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Exceptional quality! The Human EHMT2 Knockdown Cell Line in HEK293 cells has been a pivotal tool in my epigenetics research. Its efficient knockdown of EHMT2 expression has allowed me to dissect the role of histone methylation in gene regulation with precision.
Reliable and effective! The knockdown of EHMT2 in HEK293 cells has resulted in robust changes in histone methylation patterns, providing valuable insights into the epigenetic mechanisms underlying various diseases and disorders.
Streamlining my studies! With the Human EHMT2 Knockdown Cell Line, I've been able to investigate the functional consequences of EHMT2 depletion on cellular processes such as transcription, DNA repair, and cell cycle regulation. Its efficient knockdown has accelerated my research progress.
Impressive performance! The Human EHMT2 Knockdown Cell Line has consistently exhibited efficient EHMT2 silencing, allowing for the identification of EHMT2-dependent pathways and potential therapeutic targets. Its reliable knockdown has facilitated the validation of EHMT2 as a druggable target in epigenetic therapy.
A valuable research tool! The Human EHMT2 Knockdown Cell Line has significantly advanced my understanding of EHMT2 function in health and disease. Its specific knockdown of EHMT2 expression has provided a unique opportunity to explore the therapeutic potential of targeting histone methylation in various pathological conditions.
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