Cat.No. : CSC-SC003988-2 Host Cell: HepG2
| Cat. No. | CSC-SC003988-2 |
| Description | This cell line is engineered to stably express human cytochrome P450 family 7 subfamily A member 1 (CYP7A1) in HepG2 cells. |
| Gene | CYP7A1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HepG2 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Bile acids, derived from liver cholesterol, regulate lipid absorption and cholesterol balance. Researchers elucidated the crucial role of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in regulating cholesterol homeostasis and dietary lipid absorption. They discovered that estrogen-related receptor γ (ERRγ) acts as a novel transcriptional regulator of CYP7A1 expression. Activation of cannabinoid receptor type 1 (CB1 receptor) signaling induced ERRγ-mediated transcription of CYP7A1, while ERRγ overexpression increased CYP7A1 expression. Conversely, knockdown of ERRγ attenuated CYP7A1 expression. Small heterodimer partner (SHP) inhibited ERRγ's transcriptional activity, regulating CYP7A1 expression. Furthermore, the inverse agonist of ERRγ, GSK5182, reduced CYP7A1 expression and bile acid synthesis. These findings offer insights into the molecular mechanisms linking ERRγ and bile acid metabolism.
Figure 1. Researchers demonstrate that GSK5182 inhibits CYP7A1 gene expression, reducing bile acid synthesis. This effect is observed in AML12 cells through luciferase and β-galactosidase assays, qPCR, Western blot analysis, and bile acid level measurements. (Zhang Y, et al., 2022)
Using Creative Biogene's Human CYP7A1 Stable Cell Line - HepG2 can make experiments more convenient and reliable. Compared to traditional cell transfection experiments, this stable cell line ensures continuous expression of the CYP7A1 gene, avoiding the hassle of cell transfection for each experiment. This not only saves time and experimental resources but also helps reduce variability in experiments, improving the repeatability and reliability of the data. Therefore, using this stable cell line can more effectively study the expression of the CYP7A1 gene and bile acid synthesis, providing stronger support for the interpretation of experimental results.
A: HepG2 cells were likely selected due to their hepatic origin, retaining many characteristics of primary hepatocytes, making them suitable for studying CYP7A1 expression and bile acid metabolism.
A: Stability was likely confirmed through methods such as immunoblotting, qPCR, or enzyme activity assays, with continuous selection pressure applied to ensure stable maintenance of CYP7A1 expression.
A: Characterization may involve assessment of CYP7A1 enzyme activity, regulation by nuclear receptors, bile acid synthesis, and functional implications in cholesterol homeostasis and liver physiology.
A: Quality control likely included screening for mycoplasma contamination, confirmation of stable transgene integration, and assessment of phenotypic stability and consistency.
A: Comparative analysis with primary hepatocytes or in vivo models helps validate the relevance of CYP7A1 expression in bile acid synthesis, cholesterol metabolism, and liver diseases such as cholestasis and dyslipidemia.
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This Human CYP7A1 Stable Cell Line is a game-changer! Its stable expression in HepG2 cells has simplified my research on bile acid synthesis pathways.
Using this cell line feels like having a magic wand! The stable CYP7A1 expression has made studying cholesterol metabolism regulation a breeze.
Can't believe how much smoother my experiments have become with this cell line! Its reliable expression in HepG2 cells has brought clarity to my research on liver metabolism.
So impressed with this Human CYP7A1 Stable Cell Line! Its consistent expression has given me confidence in my findings and propelled my research forward.
Huge shoutout to this cell line for simplifying my work! With stable CYP7A1 expression, I can delve deeper into bile acid synthesis without any worries.
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