Cat.No. : CSC-SC003988-1 Host Cell: HeLa
| Cat. No. | CSC-SC003988-1 |
| Description | This cell line is engineered to stably express human cytochrome P450 family 7 subfamily A member 1 (CYP7A1) in HeLa cells. |
| Gene | CYP7A1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HeLa |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The initiating and rate-limiting step of the classical pathway for bile acid biosynthesis is the 7α-hydroxylation of cholesterol, a reaction catalyzed by cholesterol 7α-hydroxylase (CYP7A1). The specific aim of this study was to determine the effects of CYP7A1 overexpression on key regulatory steps of cholesterol homeostasis in hepatocytes using primary human hepatocytes (PHH) and HepG2 cells.
Here, the researchers determined the effects of CYP7A1 overexpression on several parameters of bile acid and hepatic cholesterol metabolism in primary human hepatocytes (PHH) and HepG2 cells. The data showed that overexpression of CYP7A1 in cultured cells from humans resulted in a significant increase in bile acid synthesis via the "classical" pathway. The cholesterol homeostasis response in HepG2 cells included downregulation of HMGR and upregulation of LDLR. This response suggests that overexpression of CYP7A1 in humans could be an effective approach to lower serum cholesterol.
Figure 1. Effect of overexpression of CYP7A1 on bile acid synthesis in HepG2 cell cultures. An approximately twofold increase in total bile acids synthesized by HepG2 cells was observed in the culture medium after CYP7A1 overexpression. (Pandak W M, et al., 2001)
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I've been using the Human CYP7A1 Stable Cell Line - HeLa for over six months in my research on cholesterol metabolism, and the reproducibility of my results has been outstanding. The consistency across different batches has significantly enhanced the reliability of my experimental data.
This cell line has drastically reduced the time I spend on my experiments. The stable expression of CYP7A1 means I no longer need to transfect the cells repeatedly, which has streamlined my workflow.
The versatility of the Human CYP7A1 Stable Cell Line - HeLa is impressive. We've used it in a variety of assays, including enzyme activity assays, qPCR, and Western blotting, all with exceptional results.
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