Human CYP4Z1 Stable Cell Line - HeLa

Name: Human CYP4Z1 Stable Cell Line - HeLa
SKU: CSC-SC003986-1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC003986-1

Host Cell : HeLa Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC003986-1
Description	This cell line is engineered to stably express human cytochrome P450 family 4 subfamily Z member 1 (CYP4Z1) in CHO-K1 cells.
Target Gene	CYP4Z1
Gene Species	Homo sapiens (Human)
Host Cell	HeLa
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CYP4Z1 cytochrome P450, family 4, subfamily Z, polypeptide 1 [ Homo sapiens ]
Gene Symbol	CYP4Z1
Synonyms	CYP4A20
Gene ID	199974
Uni Prot ID	Q86W10
m RNA Refseq	NM_178134.2
Protein Refseq	NP_835235.1
Chromosome Location	1p33
Function	aromatase activity; electron carrier activity; heme binding; iron ion binding;
Pathway	cytochrome P450, organism-specific biosystem; metapathway biotransformation, organism-specific biosystem;

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Background

Applications

Q & A

Customer Reviews

The Human CYP4Z1 gene encodes a member of the cytochrome P450 family of enzymes, which are responsible for the metabolism of a wide range of endogenous and exogenous compounds, including drugs, toxins, and steroids. CYP4Z1, in particular, is involved in the metabolism of fatty acids and has been implicated in the regulation of lipid homeostasis and inflammation. Variations in CYP4Z1 expression or function can affect an individual's response to medications and susceptibility to certain diseases.

The HeLa cell line, which is derived from a human cervical carcinoma, has been modified to stably express the Human CYP4Z1 gene. This stable cell line provides a valuable tool for studying the role of CYP4Z1 in drug metabolism and its potential implications in pharmacokinetics and pharmacodynamics. Researchers can use the HeLa CYP4Z1 stable cell line to investigate the enzyme's catalytic activity, its regulation by various factors, and its interaction with other components of the cytochrome P450 system. This cell line also serves as a platform for drug metabolism studies and the development of personalized medicine strategies based on individual CYP4Z1 activity.

The Human CYP4Z1 Stable Cell Line - HeLa is a cell line derived from the human cervical carcinoma cell line HeLa, engineered to express the CYP4Z1 gene. This cell line has several applications in pharmacology and toxicology:

Drug Metabolism: CYP4Z1 is involved in the metabolism of various drugs, including some anti-inflammatory and anti-cancer agents. This cell line can be used to study the role of CYP4Z1 in drug metabolism and to predict drug-drug interactions.

Toxicity Studies: The expression of CYP4Z1 can help in understanding the metabolic activation of prodrugs and the formation of toxic metabolites, which is crucial for the safety assessment of new drugs.

Cancer Research: The HeLa cell line is a well-established model for cancer research, and the addition of CYP4Z1 can provide insights into the role of this enzyme in cancer biology, particularly in the context of drug resistance and tumor microenvironment.

Customer Q&As

How is the stable expression of the CYP4Z1 gene achieved in the Human CYP4Z1 Stable Cell Line - HeLa?

A: The stable expression of the CYP4Z1 gene in the Human CYP4Z1 Stable Cell Line - HeLa is achieved by integrating the CYP4Z1 gene into the genome of HeLa cells, using selective markers and screening strategies to ensure its continuous expression. This technique typically involves the use of viral vectors, such as retroviruses or lentiviruses, along with antibiotic resistance genes as selection markers, thereby creating a cellular model for long-term study of CYP4Z1 function and drug metabolism characteristics.

What are the applications of the Human CYP4Z1 Stable Cell Line - HeLa in drug metabolism research?

A: The applications of the Human CYP4Z1 Stable Cell Line - HeLa in drug metabolism research include evaluating the metabolic properties of new drug candidates, studying the role of CYP4Z1 in drug metabolism, and exploring drug-drug interactions. This cell line provides a platform to simulate the metabolic processes in the human liver, which is crucial for predicting the metabolic fate and potential toxicity of drugs in the body.

What is the role of the Human CYP4Z1 Stable Cell Line - HeLa in personalized medicine?

A: The role of the Human CYP4Z1 Stable Cell Line - HeLa in personalized medicine is reflected in its ability to simulate the inter-individual differences in CYP4Z1 gene expression, which is significant for understanding individual drug responses and developing personalized treatment plans. With this cell line, scientists can study how CYP4Z1 gene polymorphisms affect drug metabolism, thereby providing more precise drug dosages and treatment regimens for patients.

What is the value of the Human CYP4Z1 Stable Cell Line - HeLa in studying CYP4Z1 gene polymorphisms?

A: The Human CYP4Z1 Stable Cell Line - HeLa provides an experimental platform for studying CYP4Z1 gene polymorphisms, allowing scientists to observe the impact of different genotypes on drug metabolism. This cell line helps reveal how genetic variations lead to changes in drug metabolism rates, which is significant for the assessment of drug safety and efficacy.

What is the role of the Human CYP4Z1 Stable Cell Line - HeLa in drug toxicity research?

A: The role of the Human CYP4Z1 Stable Cell Line - HeLa in drug toxicity research is to provide a model for assessing the potential toxicity of drug metabolites. By monitoring the drug metabolism process and the resulting metabolites in the cells, researchers can better understand how these metabolites affect cell function, which is crucial for predicting potential side effects and toxicity of drugs.

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Customer Reviews

Specific Drug Screening

The Human CYP4Z1 Stable Cell Line - HeLa enables researchers to conduct specific drug screening to assess the selective inhibition or activation of CYP4Z1.

Germany

2022-09-25

Metabolic Pathway Research

With the Human CYP4Z1 Stable Cell Line - HeLa, researchers can investigate the role of CYP4Z1 in drug metabolism pathways, which is crucial for new drug development.

United Kingdom

2021-12-12

Efficient Expression System

The Human CYP4Z1 Stable Cell Line - HeLa provides an efficient CYP4Z1 protein expression system for enzymology and protein engineering research.

Canada

2020-07-21

Improved Experimental Consistency

Using the Human CYP4Z1 Stable Cell Line - HeLa ensures consistency in experimental results, reducing the uncertainty due to experimental variability.

Canada

2021-01-19

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Human CYP4Z1 Stable Cell Line - HeLa

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Applications

Q & A

Customer Reviews

How is the stable expression of the CYP4Z1 gene achieved in the Human CYP4Z1 Stable Cell Line - HeLa?

What are the applications of the Human CYP4Z1 Stable Cell Line - HeLa in drug metabolism research?

What is the role of the Human CYP4Z1 Stable Cell Line - HeLa in personalized medicine?

What is the value of the Human CYP4Z1 Stable Cell Line - HeLa in studying CYP4Z1 gene polymorphisms?

What is the role of the Human CYP4Z1 Stable Cell Line - HeLa in drug toxicity research?

Specific Drug Screening

Metabolic Pathway Research

Efficient Expression System

Improved Experimental Consistency

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