Cat.No. : CSC-SC003986-1 Host Cell: HeLa
| Cat. No. | CSC-SC003986-1 |
| Description | This cell line is engineered to stably express human cytochrome P450 family 4 subfamily Z member 1 (CYP4Z1) in CHO-K1 cells. |
| Gene | CYP4Z1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HeLa |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The stable expression of the CYP4Z1 gene in the Human CYP4Z1 Stable Cell Line - HeLa is achieved by integrating the CYP4Z1 gene into the genome of HeLa cells, using selective markers and screening strategies to ensure its continuous expression. This technique typically involves the use of viral vectors, such as retroviruses or lentiviruses, along with antibiotic resistance genes as selection markers, thereby creating a cellular model for long-term study of CYP4Z1 function and drug metabolism characteristics.
A: The applications of the Human CYP4Z1 Stable Cell Line - HeLa in drug metabolism research include evaluating the metabolic properties of new drug candidates, studying the role of CYP4Z1 in drug metabolism, and exploring drug-drug interactions. This cell line provides a platform to simulate the metabolic processes in the human liver, which is crucial for predicting the metabolic fate and potential toxicity of drugs in the body.
A: The role of the Human CYP4Z1 Stable Cell Line - HeLa in personalized medicine is reflected in its ability to simulate the inter-individual differences in CYP4Z1 gene expression, which is significant for understanding individual drug responses and developing personalized treatment plans. With this cell line, scientists can study how CYP4Z1 gene polymorphisms affect drug metabolism, thereby providing more precise drug dosages and treatment regimens for patients.
A: The Human CYP4Z1 Stable Cell Line - HeLa provides an experimental platform for studying CYP4Z1 gene polymorphisms, allowing scientists to observe the impact of different genotypes on drug metabolism. This cell line helps reveal how genetic variations lead to changes in drug metabolism rates, which is significant for the assessment of drug safety and efficacy.
A: The role of the Human CYP4Z1 Stable Cell Line - HeLa in drug toxicity research is to provide a model for assessing the potential toxicity of drug metabolites. By monitoring the drug metabolism process and the resulting metabolites in the cells, researchers can better understand how these metabolites affect cell function, which is crucial for predicting potential side effects and toxicity of drugs.
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The Human CYP4Z1 Stable Cell Line - HeLa enables researchers to conduct specific drug screening to assess the selective inhibition or activation of CYP4Z1.
With the Human CYP4Z1 Stable Cell Line - HeLa, researchers can investigate the role of CYP4Z1 in drug metabolism pathways, which is crucial for new drug development.
The Human CYP4Z1 Stable Cell Line - HeLa provides an efficient CYP4Z1 protein expression system for enzymology and protein engineering research.
Using the Human CYP4Z1 Stable Cell Line - HeLa ensures consistency in experimental results, reducing the uncertainty due to experimental variability.
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