Human CLDN18-A1 Stable Cell Line - HEK293T

Name: Human CLDN18-A1 Stable Cell Line - HEK293T
SKU: CSC-RO0631

Cat.No. : CSC-RO0631 Host Cell: HEK293T

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-RO0631
Description	This cell line is derived from HEK293T and is engineered to stably overexpress Human CLDN18 isoform 1.
Gene	CLDN18-A1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Claudin 18 is a tight junction protein that plays a critical role in the regulation of paracellular transport and maintenance of tissue homeostasis. The alpha 1 isoform of Claudin 18 has been found to be upregulated in various cancers, including gastric, pancreatic, and ovarian cancer, suggesting its potential role in tumorigenesis and progression.

The Human CLDN18-A1 Stable HEK293T Cell Line can be used to study the function of hCLDN18-A1 and to investigate its role in cancer development and progression. The cell line has been characterized to ensure stable expression of hCLDN18-A1 and to minimize any unwanted effects from the HEK cell background. This cell line is a valuable tool for researchers in the field of claudin biology and may contribute to the development of new diagnostic and therapeutic strategies for cancer patients.

The Human CLDN18-A1 Stable HEK293T Cell Line is an invaluable resource for researchers investigating the role of Claudin 18 (CLDN18) in cell proliferation, migration, and apoptosis. This stable cell line, which overexpresses CLDN18, provides a controlled experimental system to study the functional significance of this tight junction protein in various physiological and pathological processes.

One key application of the Human CLDN18-A1 Stable HEK293T Cell Line is in the exploration of the mechanisms underlying CLDN18’s role in cancer development and progression. By studying these cells, researchers can gain insights into how CLDN18 expression affects tumor cell behavior, including cell proliferation, migration, and invasion.

Additionally, this cell line can be used to investigate the potential therapeutic implications of targeting CLDN18 in cancer treatment. By testing various compounds and drugs on these cells, researchers can identify potential inhibitors of CLDN18 function, which may serve as novel anti-cancer agents.

Furthermore, the Human CLDN18-A1 Stable HEK293T Cell Line can also be employed to study the role of CLDN18 in other diseases, such as neurodegenerative disorders and inflammation, as CLDN18 has been found to be dysregulated in these conditions.

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