Cat.No. : CSC-RO0631 Host Cell: HEK293T
| Cat. No. | CSC-RO0631 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Human CLDN18 isoform 1. |
| Gene | CLDN18-A1 |
| Host Cell | HEK293T |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The CLDN18-A1 gene is significant in human health as it provides instructions for making the claudin-18 protein, which is essential for the proper structure and function of tight junctions. Tight junctions are critical for maintaining the integrity of the barriers between cells in various tissues, including the digestive system and blood-brain barrier.
A: The claudin-18 protein helps to establish and regulate the blood-brain barrier by forming tight junctions between the endothelial cells that line the brain's blood vessels. This barrier restricts the passage of substances from the bloodstream into the brain, protecting it from potentially harmful compounds and maintaining a stable environment for neuronal function.
A: Mutations in the CLDN18-A1 gene can lead to dysfunctional tight junctions, which may result in increased tissue permeability and contribute to various disorders. For example, autosomal dominant medullary cystic kidney disease, type 1D (MCKD1D), is associated with mutations in this gene, leading to kidney dysfunction and eventual failure.
A: The expression of the CLDN18-A1 gene is tightly regulated in a tissue-specific manner. It is influenced by various factors, including developmental cues, cellular signaling pathways, and the presence of other proteins that interact with claudin-18. This regulation ensures that the claudin-18 protein is expressed in the appropriate tissues and at the right levels to maintain proper barrier function.
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The Human CLDN18-A1 Stable Cell Line - HEK293T comes with comprehensive technical documentation and detailed guidelines, providing researchers with the necessary resources and support to effectively utilize the cell line in their experimental setups. This ensures a smooth research experience and enhances the overall reliability of the product.
The production and use of the Human CLDN18-A1 Stable Cell Line - HEK293T adhere to strict safety and ethical standards, ensuring responsible research practices and minimizing potential risks associated with the use of genetically modified cell lines. This compliance provides reassurance for the ethical conduct of scientific research.
The Human CLDN18-A1 Stable Cell Line - HEK293T consistently yields reliable and reproducible experimental results, facilitating the validation of findings and establishing the cell line as a robust model for studying the functions and regulatory mechanisms of the CLDN18-A1 gene.
The Human CLDN18-A1 Stable Cell Line - HEK293T offers a cost-effective solution for researchers, providing a valuable tool for gene function studies without exceeding budget constraints. Its affordability enables wider accessibility and promotes its utilization across various scientific disciplines.
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