Cat.No. : CSC-SC001396-1 Host Cell: HEK293
| Cat. No. | CSC-SC001396-1 |
| Description | This cell line is engineered to stably express human 3-hydroxybutyrate dehydrogenase 1 (BDH1) in HEK293 cells. |
| Gene | BDH1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Bdh1 acts as a rate-limiting enzyme for ketone metabolism and plays an important role in metabolic regulation in the liver.However,the role of Bdh1 in MAFLD is unclear.Down-regulated expression of Bdh1 in fatty liver.In addition,palmitic acid(PA)treatment of cells also decreases Bdh1 expression.Knockdown of Bdh1 leads to ROS-induced inflammation and apoptosis,whereas overexpression of Bdh1 protects cells from lipotoxicity by inhibiting ROS overproduction.Mechanistically,Bdh1-mediatedβOHB metabolism inhibits ROS overproduction by activating Nrf2 Bdh1-mediatedβOHB metabolism inhibits ROS overproduction by enhancing the metabolic flux consisting ofβOHB-AcAc succinic acid-fumaric acid to activate Nrf2.
Figure 1.Bdh1 overexpression reversed PA-induced ROS overproduction,inflammation,and apoptosis.A.Representative western blot showing the protein level of Bdh1 in cells transfected with vector or flag-Bdh1.B.DCFH-DA probe was used to detect the level of ROS in Bdh1 overexpressing cells with indicated treatment.C.JC-1 staining showing the mitochondrial membrane potential in cells with indicated treatment.D.Representative Western blots showing the protein level of IL-1βand IL-18 in Bdh1 overexpressing cells with indicated treatment.E.mRNA levels of IL-1βand IL-18 in cells with indicated treatment.F.Representative western blots showing the protein level of cleaved caspase 3 in cells with indicated treatment.G.TUNEL assay showing the apoptosis level of Bdh1 overexpressing cells with indicated treatment.(Xu,Bu-Tuoet al.2022)
A: The BDH1 gene encodes for the 3-hydroxyacyl-CoA dehydrogenase type 1 enzyme, which is involved in the mitochondrial beta-oxidation of fatty acids. This process is crucial for energy production, particularly during fasting or prolonged exercise.
A: The BDH1 gene product, 3-hydroxyacyl-CoA dehydrogenase type 1, plays a key role in the breakdown of fatty acids, generating acetyl-CoA, which enters the citric acid cycle (TCA cycle) to produce ATP, the cell's energy currency.
A: Yes, mutations in the BDH1 gene can lead to mitochondrial trifunctional protein (MTP) deficiency, a condition characterized by the impairment of three enzymes involved in beta-oxidation. This deficiency can cause a range of symptoms, including hypoglycemia, hepatomegaly, and muscle weakness.
A: The expression of the BDH1 gene is regulated by various factors, including the availability of fatty acids and the energy state of the cell. It is also influenced by transcription factors and signaling pathways that respond to metabolic demands, ensuring that the enzyme is produced when needed for fatty acid oxidation.
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The Human BDH1 Stable Cell Line - HEK293 comes with comprehensive technical documentation and an extensive list of references, providing researchers with a solid foundation for understanding and utilizing the cell line effectively in their studies. This documentation ensures that users can quickly grasp the necessary information to optimize their experimental procedures.
The Human BDH1 Stable Cell Line - HEK293 has received positive feedback from the scientific community, with numerous case studies highlighting its utility in various research applications. This positive reception from peers further validates the cell line's reliability and effectiveness, inspiring confidence in its use for similar studies.
The supplier offers robust training and support for the Human BDH1 Stable Cell Line - HEK293, ensuring that researchers have access to the necessary resources and guidance to effectively utilize the cell line in their experimental setups. This support significantly enhances the user experience and enables researchers to maximize the potential of the cell line.
The Human BDH1 Stable Cell Line - HEK293 demonstrates high adaptability, being suitable for a wide range of experimental conditions. Additionally, the supplier's commitment to product innovation ensures that the cell line remains at the forefront of scientific research, continuously improving and adapting to the evolving needs of the research community.
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