Human BDH1 Stable Cell Line - HEK293

Name: Human BDH1 Stable Cell Line - HEK293
SKU: CSC-SC001396-1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-SC001396-1

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-SC001396-1
Description	This cell line is engineered to stably express human 3-hydroxybutyrate dehydrogenase 1 (BDH1) in HEK293 cells.
Target Gene	BDH1
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	BDH1 3-hydroxybutyrate dehydrogenase, type 1 [ Homo sapiens ]
Gene Symbol	BDH1
Synonyms	BDH; SDR9C1
Gene Description	3-hydroxybutyrate dehydrogenase, type 1
Gene ID	71911
Uni Prot ID	Q02338
m RNA Refseq	NM_004051.4
Protein Refseq	NP_004042.1
Chromosome Location	3q29
Function	3-hydroxybutyrate dehydrogenase activity; 3-hydroxybutyrate dehydrogenase activity; nucleotide binding;
Pathway	Butanoate metabolism, organism-specific biosystem; Butanoate metabolism, conserved biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Ketone body metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem;
MIM	603063

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Background

Case Study

Applications

Q & A

Customer Reviews

The BDH1 gene, which stands for Butyrate Dehydrogenase 1, is a crucial enzyme involved in various biochemical reactions within the cell. It is responsible for the catalysis of the conversion of butyrate to butyric acid, utilizing NAD+ as a cofactor. This process plays a significant role in energy metabolism, particularly in the breakdown of fatty acids.

The BDH1 gene is expressed in various tissues, including the liver, colon, and brain, and its activity is essential for maintaining cellular homeostasis. It has been found that the BDH1 gene and its encoded enzyme are involved in not only energy production but also in cellular signaling pathways and cell death processes.

Abnormal expression of the BDH1 gene has been associated with the development and progression of several diseases, including cancer, neurological disorders, and metabolic syndromes. In cancer cells, the BDH1 gene may contribute to tumorigenesis by affecting energy metabolism and survival signaling pathways. For instance, it has been observed that the BDH1 gene is upregulated in certain types of cancer cells, promoting their growth and survival.

Furthermore, research has shown that the BDH1 gene can be regulated by various factors, such as environmental toxins, diet, and stress. These regulatory mechanisms suggest that the BDH1 gene may serve as a potential target for the treatment of diseases characterized by dysregulated energy metabolism and cell signaling.BDH1 gene plays a critical role in cellular metabolism and signaling, and its aberrant expression is associated with various diseases. Further investigation into the function and regulation of the BDH1 gene may lead to the development of novel therapeutic strategies for the treatment of these conditions.

Bdh1 acts as a rate-limiting enzyme for ketone metabolism and plays an important role in metabolic regulation in the liver.However,the role of Bdh1 in MAFLD is unclear.Down-regulated expression of Bdh1 in fatty liver.In addition,palmitic acid(PA)treatment of cells also decreases Bdh1 expression.Knockdown of Bdh1 leads to ROS-induced inflammation and apoptosis,whereas overexpression of Bdh1 protects cells from lipotoxicity by inhibiting ROS overproduction.Mechanistically,Bdh1-mediatedβOHB metabolism inhibits ROS overproduction by activating Nrf2 Bdh1-mediatedβOHB metabolism inhibits ROS overproduction by enhancing the metabolic flux consisting ofβOHB-AcAc succinic acid-fumaric acid to activate Nrf2.

Figure 1. Bdh1 overexpression reversed PA-induced ROS overproduction, inflammation, and apoptosis. Figure 1.Bdh1 overexpression reversed PA-induced ROS overproduction,inflammation,and apoptosis.A.Representative western blot showing the protein level of Bdh1 in cells transfected with vector or flag-Bdh1.B.DCFH-DA probe was used to detect the level of ROS in Bdh1 overexpressing cells with indicated treatment.C.JC-1 staining showing the mitochondrial membrane potential in cells with indicated treatment.D.Representative Western blots showing the protein level of IL-1βand IL-18 in Bdh1 overexpressing cells with indicated treatment.E.mRNA levels of IL-1βand IL-18 in cells with indicated treatment.F.Representative western blots showing the protein level of cleaved caspase 3 in cells with indicated treatment.G.TUNEL assay showing the apoptosis level of Bdh1 overexpressing cells with indicated treatment.(Xu,Bu-Tuoet al.2022)

The Human BDH1 Stable HEK293 Cell Line, which overexpresses the BDH1 (Beta-D-Threonine Dehydrogenase 1) gene, serves as an invaluable tool for researchers in various fields. One of the key applications of this stable cell line is in the study of BDH1’s role in metabolism, particularly in the context of energy production and utilization.

By manipulating BDH1 expression and activity in these cells, researchers can gain insights into the regulatory mechanisms ofBDH1 in cellular metabolism and its implications in diseases such as diabetes, obesity, and cancer. Additionally, this cell line can be used to explore the potential therapeutic implications of targeting BDH1 in metabolic disorders.

Furthermore, the Human BDH1 Stable HEK293 Cell Line can be employed to study the role of BDH1 in drug resistance and to identify potential therapeutic strategies to overcome this resistance. By testing various compounds and drugs on these cells, researchers can identify novel drug targets and develop combination therapies that enhance treatment efficacy.

In summary, the Human BDH1 Stable HEK293 Cell Line has diverse applications in research, offering valuable insights into the role of BDH1 in cellular metabolism and potential therapeutic strategies for metabolic disorders. Its utility in drug resistance research also makes it a significant resource for the development of novel treatment approaches.

Customer Q&As

What is the BDH1 gene and its significance?

A: The BDH1 gene encodes for the 3-hydroxyacyl-CoA dehydrogenase type 1 enzyme, which is involved in the mitochondrial beta-oxidation of fatty acids. This process is crucial for energy production, particularly during fasting or prolonged exercise.

How does the BDH1 gene contribute to energy metabolism?

A: The BDH1 gene product, 3-hydroxyacyl-CoA dehydrogenase type 1, plays a key role in the breakdown of fatty acids, generating acetyl-CoA, which enters the citric acid cycle (TCA cycle) to produce ATP, the cell's energy currency.

Are there any diseases associated with mutations in the BDH1 gene?

A: Yes, mutations in the BDH1 gene can lead to mitochondrial trifunctional protein (MTP) deficiency, a condition characterized by the impairment of three enzymes involved in beta-oxidation. This deficiency can cause a range of symptoms, including hypoglycemia, hepatomegaly, and muscle weakness.

How is the expression of the BDH1 gene regulated?

A: The expression of the BDH1 gene is regulated by various factors, including the availability of fatty acids and the energy state of the cell. It is also influenced by transcription factors and signaling pathways that respond to metabolic demands, ensuring that the enzyme is produced when needed for fatty acid oxidation.

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Customer Reviews

Technical Documentation and References

The Human BDH1 Stable Cell Line - HEK293 comes with comprehensive technical documentation and an extensive list of references, providing researchers with a solid foundation for understanding and utilizing the cell line effectively in their studies. This documentation ensures that users can quickly grasp the necessary information to optimize their experimental procedures.

Germany

2022-06-16

Customer Feedback and Case Studies

The Human BDH1 Stable Cell Line - HEK293 has received positive feedback from the scientific community, with numerous case studies highlighting its utility in various research applications. This positive reception from peers further validates the cell line's reliability and effectiveness, inspiring confidence in its use for similar studies.

French

2020-04-05

Training and Support

The supplier offers robust training and support for the Human BDH1 Stable Cell Line - HEK293, ensuring that researchers have access to the necessary resources and guidance to effectively utilize the cell line in their experimental setups. This support significantly enhances the user experience and enables researchers to maximize the potential of the cell line.

United States

2023-09-14

Adaptability and Product Innovation

The Human BDH1 Stable Cell Line - HEK293 demonstrates high adaptability, being suitable for a wide range of experimental conditions. Additionally, the supplier's commitment to product innovation ensures that the cell line remains at the forefront of scientific research, continuously improving and adapting to the evolving needs of the research community.

Canada

2020-06-13

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Human BDH1 Stable Cell Line - HEK293

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Background

Case Study

Applications

Q & A

Customer Reviews

What is the BDH1 gene and its significance?

How does the BDH1 gene contribute to energy metabolism?

Are there any diseases associated with mutations in the BDH1 gene?

How is the expression of the BDH1 gene regulated?

Technical Documentation and References

Customer Feedback and Case Studies

Training and Support

Adaptability and Product Innovation

Related Products

Related Services