Cat.No. : CSC-RO1126 Host Cell: HepG2
| Cat. No. | CSC-RO1126 |
| Description | This cell line is engineered to stably co-express human arginase 1arginase 1 (ARG1) and human ornithine transcarbamylase (OTC) in HepG2 cells. |
| Gene | ARG1/OTC |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HepG2 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Acute liver failure (ALF) is a life-threatening condition where the liver loses its ability to detoxify harmful substances, such as bilirubin and ammonia. One approach to treat ALF is through the use of bioartificial livers (BAL), which can temporarily take over the liver's detoxification functions, giving patients a chance to recover. In this study, the researchers developed a genetically engineered version of the HepG2 cell line, called SynHeps-II, by overexpressing multiple genes involved in detoxification, including ARG1, OTC, and CPS1. This new cell line demonstrated significant improvements in detoxifying bilirubin and ammonia, both in vitro and in vivo, and also helped reduce inflammation and liver damage in animal models of ALF.
Figure 1. The researchers generated the SynHeps-I cell line by overexpressing UGT1A1 and OATP1B1 in HepG2 cells to detoxify bilirubin. They then created SynHeps-II by adding CPS1, OTC, and ARG1 to SynHeps-I cells, enabling both bilirubin and ammonia detoxification. Western blot analysis confirmed the successful expression of these genes in both cell lines. (Wang K, et al., 2024)
Our Human ARG1/OTC Stable Cell Line - HepG2 is an ideal model for studying liver detoxification mechanisms, including the metabolism of bilirubin and ammonia. This cell line can be used in similar research, providing a valuable tool for preclinical studies on ALF and liver-related diseases.
A: The Human ARG1/OTC Stable Cell Line - HepG2 is constructed by stably introducing the ARG1 (Arginase 1) and OTC (Ornithine Transcarbamylase) genes into the HepG2 liver cell line. The creation of this cell line typically involves genetic engineering techniques such as CRISPR-Cas9 or homologous recombination to ensure the continuous expression of these genes. Such a cell line is crucial for studying arginine metabolism and the urea cycle.
A: The Human ARG1/OTC Stable Cell Line - HepG2 provides an experimental model for studying urea cycle disorders, such as ornithine transcarbamylase deficiency. By simulating these genetic defects in this cell line, scientists can better understand the disease mechanisms and develop potential treatments.
A: The Human ARG1/OTC Stable Cell Line - HepG2 provides a valuable tool for studying arginine metabolism. Arginine metabolism plays a key role in various physiological processes, including protein synthesis, nitric oxide production, and cellular signaling. With this cell line, scientists can explore the regulatory mechanisms of arginine metabolism and its role in diseases.
A: The Human ARG1/OTC Stable Cell Line - HepG2 plays a significant role in studying liver diseases, particularly those related to the urea cycle and arginine metabolism. This cell line can help scientists understand the liver's function in these metabolic processes and the pathological changes during disease states.
A: The Human ARG1/OTC Stable Cell Line - HepG2 can be used to study how arginine metabolism affects cellular signal transduction. Arginine is a precursor to various signaling molecules, and its metabolic state may influence the activity of intracellular signaling pathways. With this cell line, scientists can explore the connection between arginine metabolism and cellular signal transduction, which is significant for understanding cell function and disease development.
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The Human ARG1/OTC Stable Cell Line - HepG2 ensures stable expression of ARG1 and OTC genes, which is crucial for studying metabolic pathways and liver functions.
Optimized for immediate use in experiments, the Human ARG1/OTC Stable Cell Line - HepG2 greatly reduces the preparation time for research.
Researchers can save time and resources by avoiding complex genetic manipulation in each experiment, thanks to the Human ARG1/OTC Stable Cell Line - HepG2.
The Human ARG1/OTC Stable Cell Line - HepG2 is suitable for a variety of biological and pharmacological studies, including metabolic diseases, liver disorders, and drug toxicity research.
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