Human AR Knockdown Cell Line - HEK293

The androgen receptor (AR) is a nuclear receptor that is activated by binding to androgens, testosterone, or dihydrotestosterone. AR is located in the cytoplasm and plays a crucial role in the function of the male reproductive system and the development of male secondary sexual characteristics. The AR gene is located on the X chromosome, and mutations in this gene can cause a variety of problems, such as androgen insensitivity syndrome. This receptor also plays a key role in the development and progression of prostate cancer. Overexpression or mutations in the AR gene may increase the risk of prostate cancer and determine its response to treatment. Human AR Knockdown Cell Line - HEK293 is a scientifically engineered cell line in which the AR (androgen receptor) gene has been significantly reduced or "knocked down." This cell line is derived from human embryonic kidney 293 cells, commonly referred to as HEK293. HEK293 is a special cell line that is widely used in cell biology and molecular biology research due to its ease of maintenance and robust growth in culture. AR knockdown in this cell line was achieved using small hairpin RNA (shRNA) technology. shRNA is designed to bind to AR messenger RNA (mRNA), preventing its translation into protein. This results in a significant reduction in AR protein expression, which helps elucidate the specific role and function of AR in a controlled laboratory setting.