Cat.No. : CSC-SC000765-M1 Host Cell: CHO-K1
| Cat. No. | CSC-SC000765-M1 |
| Description | This cell line is engineered to stably express human apolipoprotein A1 (APOA1) milano mutant form in CHO-K1 cells. |
| Gene | APOA1-M |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Disease research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Patients carrying the Milano mutation (R173C) in the apolipoprotein (apo) A-I gene exhibit notably reduced plasma HDL levels. Surprisingly, despite this lipid profile, they do not manifest premature atherosclerosis. The researchers conducted a comparative study of cellular lipid mobilization in human apoA-I and apoA-IMilano. They aimed to evaluate the impact of the Milano variants on lipid mobilization by measuring cholesterol and phospholipid efflux from cells to these proteins as well as rHDL particles containing them and mouse serum. The results showed that receptors containing the Milano variant showed no higher lipid efflux function in all tests compared to wild-type apoA-I and in some cases even more lipid efflux than wild-type apoA-I receptors. Outflow is lower. In addition, experimental results of cholesterol ester hydrolysis in foam cells found no difference between apoA-IMilano and apoA-Iwt. The combined findings indicate that the Milano variant of apoA-I does not exhibit greater cellular lipid mobilization than wild-type apoA-I, suggesting that the potential anti-atherosclerotic effects it provides may not be due to its promotion of cellular lipids' ability to mobilize.
Figure 1. Researchers examined the cholesterol efflux potentials of apoA-I Milano and apoA-I. In J774 macrophages that had ABCA1 upregulated by acLDL enrichment and cAMP exposure, they employed rHDLs. Milano demonstrated apoA-I-like efficiency in stimulating ABCA1-mediated efflux. (Weibel GL, et al., 2007)
The Human APOA1-M Stable Cell Line - CHO-K1 product from Creative Biogene offers a constant and dependable source of APOA1-Milano (APOA1-M) protein for in vitro research, which could improve similar trials. The effective expression of APOA1-M by this stable cell line guarantees repeatability in studies examining its potential for cholesterol efflux and other associated functions. A stable cell line provides a more standardized and controlled system than recombinant proteins, which may differ in purity and activity across batches. The cell line produces APOA1-M that is consistently high-quality and functioning, so researchers can conduct tests with confidence.
A: Human APOA1-M Stable Cell Line-CHOK1 has been tested for long periods of time and multiple passages, and the expression of APOA1-M remains stable. The expression level of APOA1M was verified by qPCR and Western Blot, and the results showed no significant changes.
A: We verified the expression of APOA1-M by qPCR and Western Blot. The qPCR results showed that the expression level of APOA1M mRNA was stable in different passages. Western Blot results showed that the expression level of APOA1M protein was consistent in each passage. Specific experimental data and maps can be provided upon request.
A: Human APOA1M Stable Cell Line-CHOK1 is suitable for APOA1-related lipoprotein research, metabolic disease research and drug screening. Functional validation included lipoprotein synthesis and secretion experiments, and the results showed that APOA1M exerts its expected functions in these processes. We also performed cell metabolism experiments to confirm its functionality.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
For this human APOA1-M stable cell line, I feel that the product supplier platform provides a good shopping experience. The product description is clear and detailed gene expression information is given, giving me a clear understanding of the product's functions. I didn't encounter any problems during the shopping process and completed the order smoothly.
Judging from the user experience, the cell growth of this human APOA1-M stable cell line is very stable. During the culture process, the cells showed consistent growth status and no abnormalities occurred. This was very helpful in my experimental work and I was able to perform cell experiments accurately.
The merchant's service is very considerate and the quality of the product is also very good, in line with my expectations.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
