Elastase I-SaCas9HF AAV (Serotype 9)

The Elastase I-SaCas9HF AAV system is a versatile and powerful tool for precision genome engineering. AAV vectors are a top choice in both the clinical and research fields due to their relatively low immunogenicity and long-term expression capacity compared to other vector systems. In addition, the non-pathogenicity of AAV and the low risk of integration into the host genome make it a safer gene therapy alternative. The Elastase I-SaCas9HF AAV utilizes the serotype 9 variant, which is known for its broad tropism and ability to effectively target multiple tissues in vivo, especially muscle, heart, and neural tissues. The vector encodes a high-fidelity version of Staphylococcus aureus Cas9, called SaCas9HF, which is a powerful genome editing tool. The expression of SaCas9 is driven by the Elastase I promoter. The Elastase I promoter is a DNA sequence located at the beginning of the Elastase I gene, which is primarily expressed in the pancreas. The Elastase I promoter controls the expression of elastase, an enzyme involved in the digestion of proteins in the small intestine. In research, the Elastase I promoter is often used in genetic engineering and studies related to gene regulation and expression, especially for targeted gene expression to the pancreas in transgenic animals.