EF1a-HPV16/E6(GFP, Puro) Lentiviral Particles

EF1a-HPV16/E6(GFP, Puro) lentiviral particles represent a cutting-edge tool in gene manipulation and cell research. This genetically engineered lentivirus expresses the HPV16 E6 oncogene under the control of the EF1a promoter, ensuring stable and sustained expression in various mammalian cell types. The vector integrates a GFP reporter gene, allowing for real-time visualization and tracking of transduced cells via fluorescence microscopy or flow cytometry. Furthermore, the introduction of a puromycin resistance gene effectively screens and enriches stably transduced cells, significantly reducing experimental background noise. This lentiviral system exhibits high transduction efficiency, stable genome integration, and the ability to infect both dividing and non-dividing cells, surpassing traditional transfection methods. Its self-inactivation (SIN) design minimizes the risk of insertional mutations, improving biosafety, while the pseudotype envelope broadens tropism, making it promising for diverse applications.

This lentiviral vector is particularly important for cell immortalization research, as the HPV16 E6 protein plays a crucial role in disrupting the p53-mediated tumor suppressor pathway. E6 promotes cell proliferation and prolongs lifespan by degrading p53, thus facilitating the establishment of immortalized cell lines from primary tissues. Researchers can utilize these viral particles to mimic carcinogenesis, study the function of viral oncoproteins, and explore mechanisms of cellular senescence. GFP labeling simplifies the monitoring of transduction efficiency and cell lineage tracking, while puromycin screening ensures cell population homogeneity, resulting in reproducible results. Its applications encompass drug screening platforms, cancer biology research, and the construction of in vitro disease models. Furthermore, this system enables long-term functional studies of the roles of HPV16 E6 in DNA damage response, apoptosis regulation, and telomerase activation, providing new insights for therapeutic interventions.